Analysis of molecular mechanism underlying ADAR2 down-regulation in ALS motor neurons

• Project/Area Number: 25253063
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neurology
• Research Institution: The University of Tokyo
• Principal Investigator: Kwak Shin 東京大学, 大学院医学系研究科(医学部), 客員研究員 (40160981)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥43,030,000 (Direct Cost: ¥33,100,000、Indirect Cost: ¥9,930,000); Fiscal Year 2016: ¥10,660,000 (Direct Cost: ¥8,200,000、Indirect Cost: ¥2,460,000); Fiscal Year 2015: ¥10,660,000 (Direct Cost: ¥8,200,000、Indirect Cost: ¥2,460,000); Fiscal Year 2014: ¥10,660,000 (Direct Cost: ¥8,200,000、Indirect Cost: ¥2,460,000); Fiscal Year 2013: ¥11,050,000 (Direct Cost: ¥8,500,000、Indirect Cost: ¥2,550,000)
• Keywords: ALS / RNA編集 / ADAR2遺伝子 / 転写因子 / FUS / 脳神経疾患 / 遺伝子 / 筋萎縮性側索硬化症 / アデノシンデアミナーゼ

Outline of Final Research Achievements

Amyotrophic lateral sclerosis (ALS) is the most common and fatal adult-onset motor neuron disease. Deficient RNA editing resulting from down-regulation of RNA editing enzyme called adenosine deaminase acting on RNA 2 (ADAR2) is closely relevant pathogenic abnormality to sporadic ALS that accounts for more than 90% of ALS patients. Because how ADAR2 gene expression is regulated is not known, we investigated the transcription factors (TFs) that specifically regulate the ADAR2 gene expression in the human motor neurons in order to elucidate the mechanism underlying ADAR2 down-regulation in ALS.
We dissected specific motor neuron tissue by laser-capture microdissection and conducted cap analysis of gene expression (CAGE) on the extracted RNA. We surveyed the TFs binding motifs in the regions expressing CAGE tags in the promoter regions of the ADAR2 gene. Several of the resulting TFs exhibited regulatory activity on the ADAR2 gene by luciferase reporter assay for the ADAR2 gene promoter.

Research Products

• [Journal Article] Calpain-dependent disruption of nucleo-cytoplasmic transport in ALS motor neurons. (2017)
  • Author(s): Yamashita T, Aizawa H, Teramoto S, Akamatsu M, Kwak S
  • Journal Title: Sci Reports Volume: 7-39994 Issue: 1
  • DOI: 10.1038/srep39994
  • Peer Reviewed / Open Access
• [Journal Article] 孤発性ALSの病態と治療－AMPA受容体阻害薬による筋萎縮性側索硬化症治療の可能性― (2017)
  • Author(s): 赤松 恵、山下 雄也、郭 伸
  • Journal Title: 神経治療学 Volume: 印刷中
  • Peer Reviewed
• [Journal Article] Ca2+-permiable AMPA receptors associated with epileptogenesis of hypothalamic hamartoma. (2017)
  • Author(s): Kitaura H, Sonoda M, Teramoto S, Shirozu H, Shimizu H, Kimura M, Masuda H, Ito Y, Takahashi H, Kwak S, Kameyama S, Kakita A.
  • Journal Title: Epilepsia Volume: 58 Issue: 4 Pages: 59-63
  • DOI: 10.1111/epi.13700
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Altered intracellular milieu of ADAR2-deficient motor neurons in amyotrophic lateral sclerosis (2017)
  • Author(s): Yamashita T, Akamatsu M, Kwak S
  • Journal Title: Genes Volume: 8(2)-60 Issue: 2 Pages: 60-60
  • DOI: 10.3390/genes8020060
  • Peer Reviewed / Open Access
• [Journal Article] The AMPA receptor antagonist perampanel robustly rescues amyotrophic lateral sclerosis (ALS) pathology in sporadic ALS model mice. (2016)
  • Author(s): Akamatsu M, Yamashita T, Hirose N, Teramoto S, Kwak S:
  • Journal Title: Sci Rep Volume: 7-28649 Issue: 1
  • DOI: 10.1038/srep28649
  • Peer Reviewed / Open Access
• [Journal Article] Anti-TIF1-γ antibody and cancer-associated myositis (2016)
  • Author(s): Hida A, Yamashita T, Kwak.S, et al
  • Journal Title: Neurology Volume: 87 Issue: 3 Pages: 299
  • DOI: 10.1212/wnl.0000000000002863
  • Peer Reviewed / Open Access
• [Journal Article] Deficient RNA-editing enzyme ADAR2 in an amyotrophic lateral sclerosis patient with a FUSP525L mutation (2016)
  • Author(s): Aizawa H, Hideyama T, Yamashita T, Kimura T, Suzuki N, Aoki M, Kwak S
  • Journal Title: J Clin Neurosci Volume: 32 Pages: 128-129
  • DOI: 10.1016/j.jocn.2015.12.039
  • Peer Reviewed / Open Access
• [Journal Article] Phosphorylated TDP-43 becomes resistant to cleavage by calpain: a regulatory role for phosphorylation in TDP-43 pathology of ALS/FTLD. (2016)
  • Author(s): Yamashita T, Teramoto S, Kwak S
  • Journal Title: Neurosci Res Volume: 未定 Pages: 63-69
  • DOI: 10.1016/j.neures.2015.12.006
  • Peer Reviewed / Open Access
• [Journal Article] 分子病態解明から治療へ (2016)
  • Author(s): 山下雄也, 郭 伸
  • Journal Title: 生体の科学 Volume: 67-4 Pages: 323-328
  • Peer Reviewed
• [Journal Article] 筋萎縮性側索硬化症－孤発性ALSモデルマウスを用いたALSの遺伝子治療法開発－ (2016)
  • Author(s): 山下雄也, 郭 伸
  • Journal Title: 遺伝子医学 MOOK Volume: 30 Pages: 257-263
  • Peer Reviewed
• [Journal Article] 神経疾患とRNA編集異常－孤発性ALSの分子病態モデルマウスを用いたALSの治療法開発 (2016)
  • Author(s): 山下雄也, 郭 伸
  • Journal Title: 生化学 Volume: 88-5 Pages: 600-608
  • Peer Reviewed
• [Journal Article] 孤発性ALSに対する遺伝子治療法の開発 (2016)
  • Author(s): 郭 伸
  • Journal Title: 臨床評価 Volume: 44-3 Pages: 588-593
• [Journal Article] Cancer association as a risk factor for anti-HMGCR antibody-positive myopathy (2016)
  • Author(s): Kadoya M, Hida A, Maeda M, Taira K, Ikenaga C, Uchio N, Kubota A, Kaida K, Miwa Y, Kurasawa K, Shimada H, Sonoo M, Chiba A, Shiio Y, Uesaka Y, Sakurai Y, Izumi T, Inoue M, Kwak S, Tsuji S, Shimizu
  • Journal Title: Neurology Volume: 3 Issue: 6 Pages: 1-10
  • DOI: 10.1212/nxi.0000000000000290
  • Peer Reviewed / Open Access
• [Journal Article] Phosphorylated TDP-43 becomes resistant to cleavage by calpain: a regulatory role for phosphorylation in TDP-43 pathology of ALS/FTLD. (2016)
  • Author(s): Yamashita T, Teramoto S, Kwak S
  • Journal Title: Neurosci Res in press Volume: -
  • Peer Reviewed
• [Journal Article] Deficient RNA-editing enzyme ADAR2 in an amyotrophic lateral sclerosis patient with a FUSP525L mutation. (2016)
  • Author(s): Aizawa H, Hideyama T, Yamashita T, Kimura T, Suzuki N, Aoki M, Kwak S
  • Journal Title: J Clin Neurosci in press Volume: -
  • Peer Reviewed
• [Journal Article] Anti-TIF1-g antibody and cancer-associated myositis:a clinicohistopathological study. (2016)
  • Author(s): Hida A, Yamashita T, Kwak S, et al.
  • Journal Title: Neurology in press Volume: -
  • Peer Reviewed
• [Journal Article] Autophagy in spinal motor neurons of conditional ADAR2-knockout mice: an implication for a role of calcium in increased autophagy flux in ALS. (2015)
  • Author(s): Sasaki S, Yamashita T, Kwak S
  • Journal Title: Neurosci Lett Volume: 598 Pages: 79-84
  • Peer Reviewed
• [Journal Article] Unique nuclear vacuoles in motor neurons of conditional ADAR2-knockout mice (2014)
  • Author(s): Sasaki S, Yamashita T, Hideyama T, Kwak S
  • Journal Title: Brain Res Volume: 1550 Pages: 536-546
  • Peer Reviewed / Open Access
• [Journal Article] The molecular link between inefficient GluA2 Q/R site-RNA editing and TDP-43 pathology in motor neurons of sporadic amyotrophic lateral sclerosis patients. (2014)
  • Author(s): Yamashita, T., and Kwak, S.
  • Journal Title: Brain Res. Volume: in press. Pages: 28-38
  • DOI: 10.1016/j.brainres.2013.12.011
  • Peer Reviewed / Open Access
• [Journal Article] TDP-43病理形成メカニズム (2014)
  • Author(s): 山下雄也, ＊郭 伸
  • Journal Title: Dementia Japan Volume: 28 Pages: 307-318
• [Journal Article] TDP-43病理形成メカニズムにおけるTDP-43のカルパイン依存性断片化の意義 (2014)
  • Author(s): 山下雄也, ＊郭 伸
  • Journal Title: 臨床神経 Volume: 54 Pages: 1151-1154
  • NAID: 130004921169
• [Journal Article] Unique nuclear vacuoles in the motor neurons of conditional ADAR2-knockout mice (2014)
  • Author(s): Sasaki S, Yamashita T, Hideyama T, Kwak S
  • Journal Title: Brain Research Volume: 1550 Pages: 36-46
  • DOI: 10.1016/j.brainres.2014.01.006
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Genetic and epigenetic studies of amhyotrophic lateral sclerosis. (2013)
  • Author(s): Al-Chalabi, A., Kwak, S., Mehler, M., Rouleau, G., Siddique, T., Strong, M., Leigh, PN.
  • Journal Title: Amyotroph Lateral Scler Frontotemporal Degener Volume: 14 Issue: sup1 Pages: 44-52
  • DOI: 10.3109/21678421.2013.778571
  • Peer Reviewed
• [Journal Article] Rescue of amyotrophic lateral sclerosis phenotype in a mouse model by intravenous AAV9-ADAR2 delivery to motor neurons (2013)
  • Author(s): Yamashita T, Chai HL, Teramoto S, Tsuji S, Shimazaki K, Muramatsu S, Kwak S
  • Journal Title: EMBO Mol Med Volume: 5(11) Issue: 11 Pages: 1710-1719
  • DOI: 10.1002/emmm.201302935
  • Peer Reviewed
• [Journal Article] A unique mouse model for investigating the properties of amyotrophic lateral sclerosis-associated protein TDP-43, by in utero electroporation. (2013)
  • Author(s): Akamatsu, M., Takuma, H., Yamashita, T., Okada, T., Keino-Masu, K., Ishii, K., Kwak, S., Masu, M., and Tamaoka, A.
  • Journal Title: Neuroscience Research Volume: 77 Issue: 4 Pages: 234-241
  • DOI: 10.1016/j.neures.2013.09.009
  • NAID: 120007130577
  • Peer Reviewed
• [Presentation] The AMPA receptor antagonist perampanel robustly rescues amyotrophic lateral sclerosis pathology in ALS model mice (2016)
  • Author(s): Akamatus M, Yamashita T, Teramoto S, Kwak S
  • Organizer: 10th FENS Forum of Neuroscience
  • Place of Presentation: Copenhagen,（Denmark)
  • Year and Date: 2016-07-02
• [Presentation] Development of specific therapy for sporadic ALS (2016)
  • Author(s): 郭 伸
  • Organizer: 【3rd World Centenarians Initiative】International Symposium on Amyotrophic Lateral Sclerosis
  • Place of Presentation: 千里ライフサイエンスセンター（大阪府豊中市）
  • Year and Date: 2016-02-19
  • Int'l Joint Research / Invited
• [Presentation] ROBUST BENEFICIAL EFFECTS OF A NON-COMPETITIVE AMPA RECEPTOR ANTAGONIST IN AN ALS MOUSE MODEL (2016)
  • Author(s): Megumi Akamatsu, Takenari Yamashita, Sayaka Teramoto, Shin Kwak
  • Organizer: The 27th International Symposium on ALS/MND
  • Place of Presentation: Convention Centre Dublin （Ireland, Dublin）
  • Int'l Joint Research
• [Presentation] A selective non-competitive AMPA receptor antagonist as a potential drug for sporadic amyotrophic lateral sclerosis (ALS) -rescue of motor dysfunctions and loss of motor neurons with TDP-43 pathology in ALS model mice (2016)
  • Author(s): Megumi Akamatsu, Takenari Yamashita, Takashi Hosaka, Naoki Hirose, Sayaka Teramoto, Shin Kwak
  • Organizer: Society for Neuroscience 2016
  • Place of Presentation: San Diego Convention Center（USA, San Diego）
  • Int'l Joint Research
• [Presentation] Death of motor neurons caused by failure of GluA2 RNA editing was robustly rescued by a selective AMPA receptor antagonist in the mechanistic ALS model mice: a potential drug for sporadic amyotrophic lateral scleroses(ALS) (2016)
  • Author(s): Megumi Akamatsu, Takenari Yamashita, Takashi Hosaka, Naoki Hirose, Sayaka Teramoto, Shin Kwak
  • Organizer: 【4th RNA Metabolism in Neurological Disease】11th Brain Research Conference
  • Place of Presentation: Paradise Point（USA, San Diego）
  • Int'l Joint Research
• [Presentation] ALSの分子標的治療 (2016)
  • Author(s): 郭 伸
  • Organizer: 【第22回日本遺伝子細胞治療学術集会】シンポジウム「Neurological and Muscle Disease 」
  • Place of Presentation: 虎ノ門ヒルズフォーラム（東京都港区）
  • Invited
• [Presentation] 筋萎縮性側索硬化症の分子標的治療 (2016)
  • Author(s): 郭 伸, 山下 雄也, 赤松 恵, 寺本 さやか
  • Organizer: 【第34回日本神経治療学会】特別プログラム「シンポジウム3」
  • Place of Presentation: 米子コンベンションセンター（鳥取県米子市）
  • Invited
• [Presentation] A role for inefficient RNA editing in the amyotrophic lateral sclerosis (ALS) pathogenesis (2015)
  • Author(s): 郭 伸
  • Organizer: 第58回日本神経化学会大会 ISN (International Society for Neurochemistry)/JSN (Japanese Society for Neurochemistry) Joint Symposium "Epigenetics in neurological and psychiatric diseases
  • Place of Presentation: 大宮ソニックシティ（埼玉県さいたま市）
  • Year and Date: 2015-09-13
  • Invited
• [Presentation] AMPA受容体アンタゴニストであるランパネルを用いたALS治療法の開発 (2015)
  • Author(s): 山下雄也, 廣瀬直己, 赤松恵, 寺本さやか, 蔡慧令, 郭伸
  • Organizer: 第38回日本神経科学大会 ワークショップ 研究ポスター「伝わる」デザイン
  • Place of Presentation: 神戸国際会議場（兵庫県神戸市）
  • Year and Date: 2015-07-29
• [Presentation] 分子病態モデルマウスを用いた筋萎縮性側索硬化症の遺伝子治療法開発 (2015)
  • Author(s): 郭 伸
  • Organizer: 第56回日本神経学会学術大会 ランチョンセミナー33「ALS研究と緩和ケアの最前線」
  • Place of Presentation: 新潟コンベンションセンター（新潟県新潟市）
  • Year and Date: 2015-05-22
  • Invited
• [Presentation] ALSのALSFRS-Rスコアの経時的変化とアクティグラフによる運動量の検討 (2015)
  • Author(s): 相澤仁志、加藤陽久、郭 伸
  • Organizer: 第56回日本神経学会学術大会 ポスター
  • Place of Presentation: 新潟コンベンションセンター（新潟県新潟市）
  • Year and Date: 2015-05-22
• [Presentation] Mechanism-based gene therapy for sporadic ALS (2015)
  • Author(s): 郭 伸、山下雄也、寺本さやか
  • Organizer: 第56回日本神経学会学術大会 Hot Topics 18 “Gene Therapy for Neurological disorders
  • Place of Presentation: 新潟コンベンションセンター（新潟県新潟市）
  • Year and Date: 2015-05-22
• [Presentation] 孤発性ALSの分子病態解析に基づいた治療法の開発 (2015)
  • Author(s): 郭 伸
  • Organizer: 京都大学iPS細胞研究所セミナー
  • Place of Presentation: 京都大学, 京都
  • Year and Date: 2015-01-23
• [Presentation] ADAR2-downregulation, a pathogenesis of ALS and a target for therapy (2014)
  • Author(s): Kwak S
  • Organizer: RNA editing-from genomic prediction to function
  • Place of Presentation: Ein Gedi, Israel
  • Year and Date: 2014-12-07 – 2014-12-10
• [Presentation] Gene therapy for sporadic ALS using an intravenous injection of AAV vector (2014)
  • Author(s): Yamashita T, Chai HL, Teramoto S, Muramatsu S, Kwak S
  • Organizer: The 25th International Symposium on MND/ALS,
  • Place of Presentation: Brussels, Belgium
  • Year and Date: 2014-12-05 – 2014-12-07
• [Presentation] Mechanism-based gene therapy for ALS using sporadic ALS model mice (2014)
  • Author(s): Yamashita T, Chai HL, Teramoto S, Tsuji S, Shimazaki K, Muramatsu S-I, Kwak S
  • Organizer: The 45th Annual Meeting Society for Neuroscience
  • Place of Presentation: Washington DC, USA
  • Year and Date: 2014-11-15 – 2014-11-19
• [Presentation] AAV-ADAR2によるALSの遺伝子治療 (2014)
  • Author(s): 郭 伸
  • Organizer: シンポジウム「AAVベクターを応用した神経・精神疾患の病態解明第36回日本生物学的精神医学会第57回日本神経化学会大会
  • Place of Presentation: 奈良
  • Year and Date: 2014-09-29 – 2014-10-01
• [Presentation] Electron microscopic observation of intranuclear aggregation of TDP-43 in mouse cerebral cortex produced by in utero electroporation (2014)
  • Author(s): Akamatsu M, Takuma H, Yamashita T, Okada T, Masu-Keino K, Oehring H, Kwak S, Masu M, Jirikowski FG
  • Organizer: 第37回日本神経科学大会Neuro2014,
  • Place of Presentation: パシフィコ横浜, 横浜
  • Year and Date: 2014-09-11 – 2014-09-13
• [Presentation] 孤発性ALSモデルマウスを用いたALSの遺伝子治療法開発の試み (2014)
  • Author(s): 山下雄也, 蔡 慧玲, 寺本さやか, 辻 省次, 島崎久仁子, 村松慎一, 郭 伸
  • Organizer: 第37回日本神経科学大会Neuro2014,
  • Place of Presentation: パシフィコ横浜, 横浜
  • Year and Date: 2014-09-11 – 2014-09-13
• [Presentation] ペランパネルはALSモデルマウス運動ニューロンのTDP-43病理を軽減する (2014)
  • Author(s): 蔡 慧玲, 郭 伸, 山下雄也
  • Organizer: 第37回日本神経科学大会Neuro2014,
  • Place of Presentation: パシフィコ横浜, 横浜
  • Year and Date: 2014-09-11 – 2014-09-13
• [Presentation] 分子病態モデルマウスを用いた筋萎縮性側索硬化症の遺伝子治療法開発 (2014)
  • Author(s): 郭 伸
  • Organizer: 第5回東京ALSフォーラム
  • Place of Presentation: シェラトン都ホテル東京, 東京
  • Year and Date: 2014-08-23
  • Invited
• [Presentation] ALS 治療への展望 (2014)
  • Author(s): 郭 伸
  • Organizer: 第12回神経難病とケアを考える会
  • Place of Presentation: エーザイホール, 東京
  • Year and Date: 2014-06-28
  • Invited
• [Presentation] Development of a gene therapy for sporadic ALS by normalizing ADAR2 activity in the motor neurons using a vascular type AAV vector (2014)
  • Author(s): Kwak S, Yamashita T, Chai HL, Teramoto S, Muramatsu S-I
  • Organizer: The 24th Meeting of the European Neurological Society
  • Place of Presentation: Istanbul, Turkey
  • Year and Date: 2014-05-31 – 2014-06-03
• [Presentation] 分子病態に基づいた孤発性ALSの遺伝子治療法の開発 (2014)
  • Author(s): 郭 伸, 山下雄也, 蔡 慧玲, 寺本さやか, 辻省次, 島崎久仁子, 村松愼一
  • Organizer: 第55回日本神経学会学術大会
  • Place of Presentation: 福岡国際会議場, 福岡
  • Year and Date: 2014-05-21 – 2014-05-24
• [Presentation] 子宮内電気穿孔法TDP-43遺伝子導入によるin vivo形成封入体の微細形態の検討 (2014)
  • Author(s): 詫間 浩, 赤松 恵, 山下雄也, Hartmut Oehring, 岡田拓也, 枡 和子, 石井一弘, Gustav Jirikowsk, 郭 伸, 枡 正幸, 玉岡 晃
  • Organizer: 第55回日本神経学会学術大会
  • Place of Presentation: 福岡国際会議場, 福岡
  • Year and Date: 2014-05-21 – 2014-05-24
• [Presentation] AMPA受容体サブユニットGluA2のQ/R部位RNA編集率に対する薬剤の効果 (2014)
  • Author(s): 澤田 潤, 相澤仁志, 浅野目明日香, 高橋佳恵, 斎藤 司, 片山隆行, 山下雄也, 郭 伸, 長谷部直幸
  • Organizer: 第55回日本神経学会学術大会,
  • Place of Presentation: 福岡国際会議場, 福岡
  • Year and Date: 2014-05-21 – 2014-05-24
• [Presentation] TDP-43病理形成メカニズムにおけるTDP-43のカルパイン依存性断片化の意義 (2014)
  • Author(s): 山下雄也, 郭 伸
  • Organizer: シンポジウム「TDP43の新展開」第55回日本神経学会学術大会
  • Place of Presentation: 福岡国際会議場, 福岡
  • Year and Date: 2014-05-21 – 2014-05-24
• [Presentation] A step for mechanism-based ALS therapy. International symposium “New Frontier of Molecular Neuropathology 2014” (2014)
  • Author(s): Kwak, S.
  • Organizer: CREST-新学術共催国際シンポジウム
  • Place of Presentation: 東京、日本
  • Invited
• [Presentation] Molecular mechanism generating TDP-43 pathology in amyotrophic lateral sclerosis (ALS) motor neurones. (2013)
  • Author(s): Kwak, S., Yamashita, T., Hideyama, T., Teramoto, S.
  • Organizer: The 23rd Meeting of the European Neurological Society.
  • Place of Presentation: Balcerona, Spain
• [Presentation] AMPA receptor-mediated activation of calpain triggers TDP-43 pathology in ALS by cleaving TDP-43 into aggregation-prone fragments. (2013)
  • Author(s): Kwak, S.
  • Organizer: GRC Calcium signaling
  • Place of Presentation: Lucca, Italy
• [Presentation] Calpain cleavage of TDP-43 in amyotrophic lateral sclerosis. Biology of Clpains in Health and Disease. (2013)
  • Author(s): Kwak, S.
  • Organizer: Science Research Conferences FASEB
  • Place of Presentation: Saxtons River, Vermont,USA
  • Invited
• [Presentation] ADAR2-dependent death and TDP-43 mislocalization in motor neurons. (2013)
  • Author(s): Kwak, S., Yamashita. T., Teramoto, S., Chai, HL.
  • Organizer: 8th Brain Research conference “RNA binding proteins in neurological disease”
  • Place of Presentation: San Diego, USA
• [Presentation] Calpain-dependent cleavage of TDP-43 plays a crucial role in ALS pathology. (2013)
  • Author(s): Yamashita, T., Hideyama, T., Teramoto, S., Takano, J., Iwata, N., Saido, TC., Kwak, S.
  • Organizer: 8th Brain Research conference “RNA binding proteins in neurological disease”
  • Place of Presentation: San Diego, USA
• [Presentation] Calpain-dependent cleavage of TDP-43 plays a crucial role in ALS pathology. (2013)
  • Author(s): Yamashita, T., Hideyama, T., Teramoto, S., Takano, J., Iwata, N., Saido, TC., Kwak, S.
  • Organizer: The 44th Annual Meeting Society for Neuroscience
  • Place of Presentation: San Diego, USA
• [Presentation] AMPA受容体サブユニットGluA2のQ/R 部位RNA編集率に対する薬剤の効果 (2013)
  • Author(s): 澤田準、相澤仁志、淺野目明日香、高橋佳恵、斉藤司、片山隆行、長谷部直幸、山下雄也、郭 伸
  • Organizer: 第54回日本神経学会学術大会
  • Place of Presentation: 東京、日本
• [Presentation] マウス胎仔電気穿孔法によるin vivo TDP-43遺伝子導入と封入体形成の検討 (2013)
  • Author(s): 詫間浩、赤松恵、山下雄也、岡田拓也、石井一弘、桝和子、郭 伸、桝正幸、玉岡晃
  • Organizer: 第54回日本神経学会学術大会
  • Place of Presentation: 東京、日本
• [Presentation] ClapainがALSのTDP-43病理形成に重要な役割を果たす (2013)
  • Author(s): 山下雄也、日出山拓人、寺本さやか、高野二郎、岩田修永、西道隆臣、郭 伸
  • Organizer: 第36回日本神経科学大会Neuro2013
  • Place of Presentation: 京都、日本
• [Presentation] ClapainがALSのTDP-43病理形成に果たす役割 (2013)
  • Author(s): 山下雄也、日出山拓人、寺本さやか、高野二郎、岩田修永、西道隆臣、郭 伸
  • Organizer: 第35回神経組織培養研究会
  • Place of Presentation: 大阪、日本
• [Book] 「ヒトの動きの神経科学シリーズ第３巻：筋力発揮の脳・神経科学―その基礎から臨床までー」筋萎縮性側索硬化症（ALS）とその治療 (2017)
  • Author(s): 郭 伸
  • Publisher: 市村出版
• [Book] 医学の歩み 247 (2013)
  • Author(s): 山下雄也、郭 伸
  • Total Pages: 9
  • Publisher: 医歯薬出版
• [Book] シリーズ＜アクチュアル脳・神経疾患の臨床＞ (2013)
  • Author(s): 郭 伸
  • Total Pages: 10
  • Publisher: 中山書店
• [Remarks] 東京大学大学院医学系研究科郭研究室
  • URL: http://newsquare.umin.ac.jp/teamkwak/index.html
• [Remarks] 東京大学大学院医学系研究科 郭 研究室
  • URL: http://square.umin.ac.jp/teamkwak/
• [Remarks] 東京大学大学院医学系研究科 疾患生命工学センター 臨床医工学部門 郭 研究室
  • URL: http://square.umin.ac.jp/teamkwak/news.html
• [Remarks] 東京大学大学院医学系研究科 疾患生命工学センター 臨床医工学部門 郭研究室
  • URL: http://square.umin.ac.jp/teamkwak