| Project/Area Number |
25253066
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neurology
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| Research Institution | Doshisha University (2014-2015)
Juntendo University (2013) |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
Miyazaki Haruko 独立行政法人理化学研究所, 視床発生研究チーム, 研究員 (80525890)
下郡 智美 独立行政法人理化学研究所, 視床発生研究チーム, チームリーダー (30391981)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥45,110,000 (Direct Cost: ¥34,700,000、Indirect Cost: ¥10,410,000)
Fiscal Year 2015: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
Fiscal Year 2014: ¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2013: ¥19,110,000 (Direct Cost: ¥14,700,000、Indirect Cost: ¥4,410,000)
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| Keywords | ポリグルタミン病 / 遺伝子発現 / lncRNA / 非コードRNA / 中型有棘神経細胞 / lincRNA |
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| Outline of Final Research Achievements |
To reveal the pathological significance of the gene expression abnormality in Huntington disease(HD), we developed the method to identify the gene expression changes in specific neuronal fraction such as medium spiny neurons(MSN) in striatum. We isolated MSN using cell sorter from control and HD model mice with Venus expression in MSN, then compared their gene expressions with microarray. We identified many genes, which showed expression changes, those include previously reported and unreported ones.Those could be confirmed by RT-PCR if the expression levels are above the certain level. We also identified the expression abnormality of long non-coding RNA, however, many of them showed low expression level. Thus, new methods for confirmation is necessary.
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