Generation of pancreatic islet-like tissue by using an innovative 3D-culture method.
Project/Area Number |
25253079
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Research Category |
Grant-in-Aid for Scientific Research (A)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
General surgery
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Research Institution | Yokohama City University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
関根 圭輔 横浜市立大学, 医学部, 助教 (00323569)
武部 貴則 横浜市立大学, 医学部, 准教授 (20612625)
小池 直人 横浜市立大学, 医学(系)研究科(研究院), 客員教授 (50301081)
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Project Period (FY) |
2013-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥44,590,000 (Direct Cost: ¥34,300,000、Indirect Cost: ¥10,290,000)
Fiscal Year 2016: ¥10,400,000 (Direct Cost: ¥8,000,000、Indirect Cost: ¥2,400,000)
Fiscal Year 2015: ¥10,400,000 (Direct Cost: ¥8,000,000、Indirect Cost: ¥2,400,000)
Fiscal Year 2014: ¥11,310,000 (Direct Cost: ¥8,700,000、Indirect Cost: ¥2,610,000)
Fiscal Year 2013: ¥12,480,000 (Direct Cost: ¥9,600,000、Indirect Cost: ¥2,880,000)
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Keywords | 膵臓 / 膵島移植 / 三次元培養 / 血管 / iPS細胞 / 内胚葉前駆細胞 / 糖尿病 / 膵島 / 分化誘導 / 膵前駆細胞 |
Outline of Final Research Achievements |
We are developing a scaffold-free 3D-human organ bud generation method from reproduction of interaction with endothelial and mesenchymal cells mimicking initial process of organogenesis (Nature 499:481-484, 2013). Adapting this 3D-culture system, we have tried to develop a cell manipulation technique to artificially build a 3D pancreatic islet tissue using pancreas β cells. We have succeeded in condensation of multiple pancreatic islet and forming vascular-like structures around it by developing multiple clustering technology and promotion technology of vascularization targeting mice and human pancreatic islet. By transplanting this vascularized islet to immune-deficient mice, the same level of vasculature as pancreatic islet in vivo is maintained inside the vascularized pancreatic islet. The vascularized islets was transplanted into fulminant type 1 diabetes mellitus modeled mice resulted in significant improvement of survival rate of these mice.
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Report
(5 results)
Research Products
(94 results)
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[Journal Article] Vascularized and Complex Organ Buds from Diverse Tissues via Mesenchymal Cell-Driven Condensation2015
Author(s)
T. Takebe, M. Eomura, E. Yoshizawa, M. Kimura, H. Koike, Y. Ueno, T. Matsuzaki, T. Yamazaki, T. Toyohara, K. Osafune, H. Nakauchi, H. Y. Yoshikawa, H. Taniguchi
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Journal Title
Cell Stem Cell
Volume: 16
Issue: 5
Pages: 556-565
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Polycomb group protein Ezh2 regulates hepatic progenitor cell proliferation and differentiation in murine embryonic liver.2014
Author(s)
Koike H, Ouchi R, Ueno Y, Nakata S, Obana Y, Sekine K, Zheng YW, Takebe T, Isono K, Koseki H, Taniguchi H.
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Journal Title
PLoS One.
Volume: 9
Issue: 8
Pages: 0-2
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Efficient hepatic differentiation of human induced pluripotent stem cells in a three-dimensional microscale culture.2014
Author(s)
Zhang RR, Takebe T, Miyazaki L, Takayama M, Koike H, Kimura M, Enomura M, Zheng YW, Sekine K, Taniguchi H.
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Journal Title
Methods Mol Biol.
Volume: 1210
Pages: 131-141
DOI
ISBN
9781493914340, 9781493914357
Related Report
Peer Reviewed
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[Journal Article] The evidence of sophisticatedly heterogeneous population of human umbilical vein endothelial cells2014
Author(s)
Zheng YW, Nie YZ, Tsuchida T, Zhang RR, Aoki K, Sekine K, Ogawa M, Takebe T, Ueno Y, Sakakibara H, Hirahara F, Taniguchi H
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Journal Title
Transplant Proc
Volume: 46
Issue: 4
Pages: 1251-3
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Ring1B Promotes Hepatic Stem/Progenitor Cell Expansion via Simultaneous Suppression of Cdkn1a and Cdkn2a.2014
Author(s)
Koike H, Ueno Y, Naito T, Shiina T, Nakata S, Ouchi R, Obana Y, Sekine K, Zheng YW, Takebe T, Isono K, Koseki H, Taniguchi H
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Journal Title
Hepatology
Volume: 印刷中
Issue: 1
Pages: 323-333
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Vascularized and functional human liver from an iPSC-derived organ bud transplant.2013
Author(s)
Takanori Takebe*, Keisuke Sekine, Masahiro Enomura, Hiroyuki Koike, Ran-Ran Zhang, Yasuharu Ueno, Yun-Wen Zheng, Naoto Koike, Shinsuke Aoyama, Yasuhisa Adachi, Hideki Taniguch*(*: Correspondence)
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Journal Title
Nature
Volume: 499
Issue: 7459
Pages: 481-484
DOI
NAID
Related Report
Peer Reviewed
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[Presentation] 肝芽の同所性移植モデルの確立2016
Author(s)
土田倫範, 村田聡一郎, 小林真史, 聶運中, 武部貴則, 鄭允文, 岡本理志,谷口英樹
Organizer
第15回日本再生医療学会総会
Place of Presentation
大阪国際会議場(大阪府大阪市)
Year and Date
2016-03-17
Related Report
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[Presentation] Creation of vascularized and functional human liver from an induced pluripotent stem cell-derived organ bud transplant.2013
Author(s)
Takebe T, Sekine K, Enomura M, Koike H, Zhang R, Kimura M, Ogaeri T, Yoshizawa E, Kawamata F, Koike N, Ueno Y, Zheng YW, Taniguchi H
Organizer
The 13th Congress of the Asian Society of Transplantation.
Place of Presentation
国立京都国際会館(京都府)
Related Report
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[Presentation] Flow cytometric identification and characterization of endothelial progenitor cells in human umbilical vein endothelial cells.2013
Author(s)
Zheng YW, Aoki K, Tsuchida T, Zhang R, Sekine K, Ogawa M, Takebe T, Yasumura K, Ueno Y, Sakakibara H, Hiranaka F, Taniguchi H
Organizer
The 13th Congress of the Asian Society of Transplantation.
Place of Presentation
国立京都国際会館(京都府)
Related Report
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