| Budget Amount *help |
¥44,590,000 (Direct Cost: ¥34,300,000、Indirect Cost: ¥10,290,000)
Fiscal Year 2016: ¥10,400,000 (Direct Cost: ¥8,000,000、Indirect Cost: ¥2,400,000)
Fiscal Year 2015: ¥10,400,000 (Direct Cost: ¥8,000,000、Indirect Cost: ¥2,400,000)
Fiscal Year 2014: ¥11,310,000 (Direct Cost: ¥8,700,000、Indirect Cost: ¥2,610,000)
Fiscal Year 2013: ¥12,480,000 (Direct Cost: ¥9,600,000、Indirect Cost: ¥2,880,000)
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| Outline of Final Research Achievements |
We are developing a scaffold-free 3D-human organ bud generation method from reproduction of interaction with endothelial and mesenchymal cells mimicking initial process of organogenesis (Nature 499:481-484, 2013). Adapting this 3D-culture system, we have tried to develop a cell manipulation technique to artificially build a 3D pancreatic islet tissue using pancreas β cells. We have succeeded in condensation of multiple pancreatic islet and forming vascular-like structures around it by developing multiple clustering technology and promotion technology of vascularization targeting mice and human pancreatic islet. By transplanting this vascularized islet to immune-deficient mice, the same level of vasculature as pancreatic islet in vivo is maintained inside the vascularized pancreatic islet. The vascularized islets was transplanted into fulminant type 1 diabetes mellitus modeled mice resulted in significant improvement of survival rate of these mice.
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