| Project/Area Number |
25253089
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Hiroshima University |
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Principal Investigator |
OCHI MITSUO 広島大学, その他部局等, 学長 (70177244)
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| Co-Investigator(Kenkyū-buntansha) |
ADACHI NOBUO 広島大学, 大学院医歯薬保健学研究院, 教授 (30294383)
MIYAKI SHIGERU 広島大学, 病院, 講師 (10392490)
KAMEI NAOSUKE 広島大学, 病院, 講師 (70444685)
NAKASA TOMOYUKI 広島大学, 病院, 病院助教 (60467769)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥44,980,000 (Direct Cost: ¥34,600,000、Indirect Cost: ¥10,380,000)
Fiscal Year 2015: ¥10,270,000 (Direct Cost: ¥7,900,000、Indirect Cost: ¥2,370,000)
Fiscal Year 2014: ¥12,350,000 (Direct Cost: ¥9,500,000、Indirect Cost: ¥2,850,000)
Fiscal Year 2013: ¥22,360,000 (Direct Cost: ¥17,200,000、Indirect Cost: ¥5,160,000)
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| Keywords | 組織再生 / 間葉系幹細胞 / エクソソーム / microRNA / miRNA |
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| Outline of Final Research Achievements |
Paracrine signaling by bone-marrow-derived mesenchymal stem cells (MSCs) plays a major role in tissue repair. We focused on exosomes, which are extracellular vesicles as a novel additional modulator of cell-to-cell communication and tissue regeneration. To address this, we examined the role of exosomes in the healing process in injury models of deficient mice, a strain which is known to reduce levels and/or function of exosomes. The retardation of tissue repair in deficient mice was rescued by the injection of MSC-exosomes. MSC-exosomes promoted cell differentiation such as myogenesis and angiogenesis in vitro, and tissue regeneration in tissue injury models. The levels of the tissue repair related-cytokines in MSC-exosomes were low, suggesting that, tissue repair may be in part mediated by other MSC-exosome components, such as miRNAs. We conclude that MSC-exosomes are a novel factor of MSC paracrine signaling with an important role in the tissue repair process.
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