| Project/Area Number |
25253101
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Conservative dentistry
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| Research Institution | Meikai University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
Kawashima Nobuyuki 東京医科歯科大学, 医歯(薬)学総合研究科, 助教 (60272605)
Nishimura Fusanori 広島大学, 医歯(薬)学総合研究科, 教授 (80208222)
Izumi Toshio 福岡歯科大学, 歯学部, 准教授 (40248547)
Kanazashi Mikimoto 神奈川歯科大学, 歯学部, 講師 (80339811)
Ozeki Nobuaki 愛知学院大学, 歯学部, 講師 (70469005)
Ikeda Takashi 長崎大学, 大学病院, 講師 (90244079)
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| Project Period (FY) |
2013-10-21 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥42,380,000 (Direct Cost: ¥32,600,000、Indirect Cost: ¥9,780,000)
Fiscal Year 2015: ¥11,830,000 (Direct Cost: ¥9,100,000、Indirect Cost: ¥2,730,000)
Fiscal Year 2014: ¥12,870,000 (Direct Cost: ¥9,900,000、Indirect Cost: ¥2,970,000)
Fiscal Year 2013: ¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
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| Keywords | 歯髄組織 / 再生療法 / 象牙質形成 / Ectodin / 生体活性ガラス / スフェロイド / ES細胞 / レーザー / 歯髄再生療法 / 歯髄幹細胞 / 生体ガラス / 歯髄細胞 / 象牙芽細胞 / 細胞分化 / ectodin / wntシグナル / 3次元培養 / Spheroid / 三次元培養法 / ＥＳ細胞 / iPS細胞 |
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| Outline of Final Research Achievements |
This study presented the following conclusions: 1 Dental pulp tissue contains stem cells which can apply for regenerative dental pulp therapy. Therefore, we established culture system to isolate the stem cells from dental pulp tissue and confirmed that the stem cells differentiated into mature odontoblasts producing mineralized dentin matrix. 2 We established the methods to make ES cells differentiate into mature odontoblasts and to apply the cells for regenerative dental pulp therapy. 3 In order to attempt to develop regenerative dental pulp therapy, we tried to use functional glass beads and fish collagen as the biomaterials for scaffold of the stem cells. The stem cells included in the scaffold differentiated into mature odontoblasts which produced abundant mineralized dentin matrix. These results can contribute to development of regenerative dental pulp therapy in clinical application.
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