| Budget Amount *help |
¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000)
Fiscal Year 2015: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2014: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2013: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
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| Outline of Final Research Achievements |
We have developed a dynamic model of the central metabolism (including glycolysis, TCA cycle, pentose phosphate pathways, and anaplerotic reactions) which is responsible for material conversion and energy metabolism. This is the largest-scale kinetic model for a batch culture and is a base for synthetic biology to aim at a design of cell functions. We integrated gene regulation networks that perform glucose uptake and intracellular metabolic changes into the central carbon metabolism and predicted the dynamic behaviors under different environmental and genetic conditions. It is employed to rationally design a microbial system for enhanced production of useful compounds and to understand how complex metabolisms are regulated at the molecular interaction level.
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