Alternative DNA repair pathways of DNA damage induced by topoisomerase inhibitor
| Project/Area Number |
25281018
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Risk sciences of radiation and chemicals
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| Research Institution | Osaka University |
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Principal Investigator |
Kuraoka Isao 大阪大学, 基礎工学研究科, 准教授 (60335396)
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| Co-Investigator(Renkei-kenkyūsha) |
Iwai Shigenori 大阪大学, 大学院基礎工学研究科, 教授 (10168544)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
Fiscal Year 2015: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2014: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2013: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
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| Keywords | トポイソメラーゼ阻害剤 / 癌 / DNA修復 / ヌクレアーゼ / がん |
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| Outline of Final Research Achievements |
Human topoisomerase inhibitors are effective against a broad spectrum of tumors. They induce DNA damage leading cell death by the inhibition of enzymatic topoisomerase reaction specifically.
Here, we studied the repair mechanism of topoisomerase-induced DNA damage in human cells. We found that ERCC1-XPF and RPA participates in DNA repair of the Top1-DNA damage complex and that FEN1 participates in repair of the 5'-phosphotyrosyl terminus of DNA single-strand breaks. Our data provided insights into the DNA repair mechanisms in tumor cells.
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Report
(4 results)
Research Products
(28 results)
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[Journal Article] The SLX4 complex is a SUMO E3 ligase that impacts on replication stress outcome and genome stability.2015
Author(s)
Guervilly JH, Takedachi A, Naim V, Scaglione S, Chawhan C, Lovera Y, Despras E, Kuraoka I, Kannouche P, Rosselli F, Gaillard PH.
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Journal Title
Mol Cell.
Volume: 57
Issue: 1
Pages: 123-137
DOI
Related Report
Peer Reviewed
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[Journal Article] Guanine-5-carboxylcytosine base pairs mimic mismatches during DNA replication.2014
Author(s)
Shibutani T, Ito S, Toda M, Kanao R, Collins LB, Shibata M, Urabe M, Koseki H, Masuda Y, Swenberg JA, Masutani C, Hanaoka F, Iwai S, Kuraoka I
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Journal Title
Scientific Reports
Volume: 4
Issue: 1
Pages: 5220-5220
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Fluorescence detection of cellular nucleotide excision repair of damaged DNA.2014
Author(s)
Toga, T., Kuraoka, I., Watanabe, S., Nakano, E., Takeuchi, S., Nishigori, C., Sugasawa, K. and Iwai, S.
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Journal Title
Scientific Reports
Volume: 4
Issue: 1
Pages: 5578-5578
DOI
Related Report
Peer Reviewed / Open Access
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