| Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2015: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
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| Outline of Final Research Achievements |
Genetic instability induced by radiation or chemical mutagens is an interesting phenomenon and plays an important role on carcinogenesis, which appear through long latency period. The factor based under these phenomena is considered to "damage memory", however its entity is not clear. Based on several reports, we hypothesized an epigenetic events such as DNA and histone modification significantly contribute to the "damage memory". In this study, to test the hypothesis we have established a system for detecting the genetic instability. For inducing double strand breaks (DSBs) we used CRISPR, by which we can introduce DSBs at any genomic sequence we want and for the target of genetic instability that on microsatellite was tested. In addition, we have constructed BAC vector containing the entire genome region of ATR or DNA-PKcs gene for the rescue of each mutant, respectively.
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