Androgen-induced signaling and sexual differentiation of reproductive organs
| Project/Area Number |
25281026
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Risk sciences of radiation and chemicals
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| Research Institution | Gifu Pharmaceutical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
永瀬 久光 岐阜薬科大学, 薬学部, 教授 (40141395)
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2016: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2015: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
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| Keywords | アンドロゲン / 性分化 / 17β-ヒドロキシステロイド脱水素酵素 / 胎生期・発達期影響 / トランスジェニックマウス |
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| Outline of Final Research Achievements |
Androgens play a major role in male sexual development. Although androgen-induced masculinization has been well studied, genuine role of androgens in reproductive development remains unclear. To study the direct effect of androgens in reproductive development, we generated a transgenic mouse that carries a transgene expressing EGFP-tagged 17β-hydroxysteroid dehydrogenase type 3 (17β3E), which converts androstenedione into testosterone, based on the Cre/loxP recombination (17β3ETG mouse). When 17β3ETG mice were mated with Cre-expressing TG mice, testosterone and dihydrotestosterone levels in the 17β3E/Cre double TG fetuses (DTG) were significantly higher than those of the 17β3E single TG littermates in both male and female. Consistent with these results, female DTG has formed male reproductive tracts in the prenatal and postnatal period. These results suggest that our model mice are potential tool for investigating genuine role of androgens in reproductive development.
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Report
(5 results)
Research Products
(51 results)
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[Journal Article] Germline recombination in a novel Cre transgenic line, Prl3b1-cre mouse.2016
Author(s)
Al-Soudy AS, Hiromori Y, Mizuno S, Hasegawa Y, Shawki HH, Katoh MC, Basha WA, Ibrahim AE, El-Shemy HA, Iseki Hi, Yoshiki A, Nagase H, Nakanishi T, Takahashi S, Oishi H, Sugiyama F.
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Journal Title
Genesis.
Volume: 54(7)
Issue: 7
Pages: 389-97
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Structural basis for PPARgamma transactivation by endocrine disrupting organotin compounds.2015
Author(s)
Harada, S., Hiromori, Y., Nakamura, S., Kawahara, K., Fukakusa, S., Maruno, T., Noda, M., Uchiyama, S., Fukui, K., Nishikawa, J., Nagase, H., Kobayashi, Y., Yoshida, T., Ohkubo, T., Nakanishi, T.,
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Journal Title
Sci. Rep.
Volume: 5:8520
Issue: 1
Pages: 1-7
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] A mollusk retinoic acid receptor (RAR) ortholog sheds light on the evolution of ligand binding2014
Author(s)
Gutierrez-Mazariegos J, Kumar Nadendla E, Lima D, Kane M, Nishikawa J, Hiromori Y, Nakanishi T, Santos MM, Castro LFC, Bourguet W, Schubert M, Laudet V
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Journal Title
Endocrinology
Volume: 155
Issue: 11
Pages: 4275-4286
DOI
Related Report
Peer Reviewed
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[Presentation] 卵巣機能によるTPT毒性発現修飾2014
Author(s)
大塚佑基、青木 明、中西 剛、永瀬久光
Organizer
第４回メタロミクス研究フォーラム
Place of Presentation
武蔵野大学（東京都西東京市）
Year and Date
2014-11-07 – 2014-11-08
Related Report
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