| Budget Amount *help |
¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2016: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2015: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
|
|---|
| Outline of Final Research Achievements |
Androgens play a major role in male sexual development. Although androgen-induced masculinization has been well studied, genuine role of androgens in reproductive development remains unclear. To study the direct effect of androgens in reproductive development, we generated a transgenic mouse that carries a transgene expressing EGFP-tagged 17β-hydroxysteroid dehydrogenase type 3 (17β3E), which converts androstenedione into testosterone, based on the Cre/loxP recombination (17β3ETG mouse). When 17β3ETG mice were mated with Cre-expressing TG mice, testosterone and dihydrotestosterone levels in the 17β3E/Cre double TG fetuses (DTG) were significantly higher than those of the 17β3E single TG littermates in both male and female. Consistent with these results, female DTG has formed male reproductive tracts in the prenatal and postnatal period. These results suggest that our model mice are potential tool for investigating genuine role of androgens in reproductive development.
|
