Studies on quantitative evaluation of inherited germline mutations
| Project/Area Number |
25281027
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Risk sciences of radiation and chemicals
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| Research Institution | National Institute of Health Sciences |
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Principal Investigator |
Masumura Kenichi 国立医薬品食品衛生研究所, 変異遺伝部, 室長 (40291116)
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| Co-Investigator(Renkei-kenkyūsha) |
NOHMI Takehiko 国立医薬品食品衛生研究所, 安全性生物試験研究センター, 客員研究員 (30150890)
HONMA Masamitsu 国立医薬品食品衛生研究所, 変異遺伝部, 部長 (30250179)
TOYODA Naomi (HOKAIWADO Naomi) 国立医薬品食品衛生研究所, 変異遺伝部, 非常勤職員 (70381853)
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
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| Keywords | 遺伝毒性 / ゲノム / 次世代影響 / 遺伝子突然変異 / 環境変異原 / 次世代シークエンサー / 生殖細胞突然変異 / トランスジェニックマウス遺伝子突然変異試験 |
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| Outline of Final Research Achievements |
De novo germline mutations and their health effects in next generation are important in genetic toxicology. Inherited germline mutations in mice induced by N-ethyl-N-nitrosourea (ENU) were analyzed by whole-exome sequencing. Male gpt delta mice were treated with ENU and mated with untreated females. ENU-treated and control families were used for whole-exome sequencing. Single nucleotide variants (SNVs) and de novo mutations in the offspring were detected. Frequencies of inherited mutations increased with dosage. The result suggests that direct sequencing analysis may be a useful tool to investigate inherited germline mutations induced by environmental mutagens.
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Report
(5 results)
Research Products
(46 results)
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[Journal Article] Role of p53 in the progression from ochratoxin A-induced DNA damage to gene mutations in the kidneys of mice.2015
Author(s)
Kuroda K, Hibi D, Ishii Y, Yokoo Y, Takasu S, Kijima A, Matsushita K, Masumura K, Kodama Y, Yanai T, Sakai H, Nohmi T, Ogawa K, Umemura T.
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Journal Title
Toxicol Sci.
Volume: 144
Issue: 1
Pages: 65-76
DOI
Related Report
Peer Reviewed
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[Journal Article] In vivo evidence that DNA polymerase kappa is responsible for error-free bypass across DNA cross-links induced by mitomycin C.2014
Author(s)
Takeiri A, Wada NA, Motoyama S, Matsuzaki K, Tateishi H, Matsumoto K, Niimi N, Sassa A, Grúz P, Masumura K, Yamada M, Mishima M, Jishage KI, Nohmi T.
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Journal Title
DNA Repair
Volume: 24
Pages: 113-121
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Ochratoxin A induces DNA double-strand breaks and large deletion mutations in the carcinogenic target site of gpt delta rats2014
Author(s)
K. Kuroda, D. Hibi, Y. Ishii, S. Takasu, A. Kijima, K. Matsushita, K. Masumura, M. Watanabe, Y. Sugita-Konishi, H. Sakai, T. Yanai, T. Nohmi, K. Ogawa and T. Umemura
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Journal Title
Mutagenesis
Volume: 29
Issue: 1
Pages: 27-36
DOI
Related Report
Peer Reviewed
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[Presentation] Sensitivity of inactivated DNA polymerase kappa knock-in mice to mitomycin C2015
Author(s)
Takeiri A, Naoko AW, Motoyama S, Matsuzaki K, Tateishi H, Matsumoto K, Niimi N, Sassa A, Grúz P, Masumura K, Yamada M, Mishima M, Jishage K, Nohmi T
Organizer
The 7th International Congress of Asian Society of Toxicology
Place of Presentation
International Convention Center Jeju Island, South Korea
Year and Date
2015-06-23
Related Report
Int'l Joint Research
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[Presentation] Spontaneous point mutations and deletions increased with aging in gpt delta transgenic mice and rats2013
Author(s)
Masumura K, Toyoda-Hokaiwado N, Osugi N, Ishii Y, Umemura T, Takagi H, Nishikawa A, Nohmi T, Honma M
Organizer
11th International Conference on Environmental Mutagens
Place of Presentation
Bourbon Cataratas Convention & Spa Resort, Foz do Iguazu, Brazil
Related Report
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