Development of reporter mice enabling detection of epigenetic toxicity in the conventional toxicity tests
| Project/Area Number |
25281028
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Risk sciences of radiation and chemicals
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| Research Institution | Hoshi University (2014-2017)
National Institute of Health Sciences (2013) |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
大塚 まき 星薬科大学, 先端生命科学研究所, 特任助教 (40734372)
山本 直樹 星薬科大学, 先端生命科学研究所, 特任助教 (50757432)
大久保 佑亮 国立医薬品食品衛生研究所, 毒性部, 研究員 (80596247)
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| Project Period (FY) |
2013-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
Fiscal Year 2016: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2015: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
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| Keywords | ゲノム / エピジェネティクス / 毒性試験 / レポーター / 有害化学物質 / トキシコロジー / エピジェネティック毒性 / レポーターマウス |
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| Outline of Final Research Achievements |
Regardless of its importance, risk management for epigenetic toxicity has not been advanced. One of the reasons is the high degree of difficulty of its detection. In this study, we developed a reporter mouse that enables simple detection of epigenetic toxicity. Construction of the reporter vector was the most difficult, but adopting the Snrpn promoter derived from the imprint gene, we could construct the vectors joining the promoter sequence of Agouti or Daz1 to the upstream of Snrpn promoter. We introduced both reporter vectors to culture cell and confirmed that both show the expected reporter response. We next examined the response to DNA demethylating substances and confirmed the enhancement of both reporter response. Following this result, we moved to the production of the reporter mice (outsourcing) and carried out detailed analysis of the produced mice.
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Report
(6 results)
Research Products
(22 results)
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[Journal Article] Reduced Adult Hippocampal Neurogenesis and Cognitive Impairments following Prenatal Treatment of the Antiepileptic Drug Valproic Acid2015
Author(s)
Juliandi B, Tanemura K, Igarashi K, Tominaga T, Furukawa Y, Otsuka M, Moriyama N, Ikegami D, Abematsu M, Sanosaka T, Tsujimura K, Narita M, Kanno J, Nakashima K
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Journal Title
Stem Cell Rep
Volume: 5
Issue: 6
Pages: 996-1009
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Identification of myelin transcription factor 1 (MyT1) as a subunit of the neural cell type-specific lysine-specific demethylase 1 (LSD1) complex.2014
Author(s)
Yokoyama A, Igarashi K, Sato T, Takagi K, Otsuka I M, Shishido Y, Baba T, Ito R, Kanno J, Ohkawa Y, Morohashi K, Sugawara A.
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Journal Title
J Biol Chem.
Volume: 289
Issue: 26
Pages: 18152-18162
DOI
Related Report
Peer Reviewed
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