| Budget Amount *help |
¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
Fiscal Year 2016: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2015: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
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| Outline of Final Research Achievements |
Regardless of its importance, risk management for epigenetic toxicity has not been advanced. One of the reasons is the high degree of difficulty of its detection. In this study, we developed a reporter mouse that enables simple detection of epigenetic toxicity. Construction of the reporter vector was the most difficult, but adopting the Snrpn promoter derived from the imprint gene, we could construct the vectors joining the promoter sequence of Agouti or Daz1 to the upstream of Snrpn promoter. We introduced both reporter vectors to culture cell and confirmed that both show the expected reporter response. We next examined the response to DNA demethylating substances and confirmed the enhancement of both reporter response. Following this result, we moved to the production of the reporter mice (outsourcing) and carried out detailed analysis of the produced mice.
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