The development of systems for evaluation of biodiversity and functioning of soil animals based on next-gereration sequencing analysis

• Project/Area Number: 25281029
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Environmental impact assessment
• Research Institution: Yokohama National University
• Principal Investigator: Nakamori Taizo 横浜国立大学, 大学院環境情報研究院, 准教授 (50443081)
• Co-Investigator(Kenkyū-buntansha): 齋藤 星耕 琉球大学, 熱帯生物圏研究センター, 研究員 (10623754) ; 青山 洋昭 琉球大学, 熱帯生物圏研究センター, 研究員 (20468078) ; 金子 信博 横浜国立大学, 環境情報研究科(研究院), 教授 (30183271) ; 藤井 佐織 横浜国立大学, 環境情報研究院, 産学連携研究員 (50648045)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000); Fiscal Year 2016: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000); Fiscal Year 2015: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2014: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2013: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
• Keywords: DNAバーコード / 生態系影響評価 / 次世代シーケンス / 土壌動物 / 環境評価

Outline of Final Research Achievements

This project contributed to the development of methods for evaluating species, phylogenetic, and functional diversities from community samples of Collembola, a major group of soil arthoropods, based on next-generation sequencing. DNA barcoding was performed for about 100 species and their phylogenetic tree was estimated. Trait data were obtained by literature surveys and laboratory and field observations for some species. A quantitative protocol for community analysis using next-generation sequencing was provided.

Research Products

• [Int'l Joint Research] Biotechnology Center of Hochiminh City(ベトナム)
• [Int'l Joint Research] Moscow State Pedagogical University(ロシア連邦)
• [Int'l Joint Research] Biotechnology Center of Hochiminh City(ベトナム)
• [Journal Article] A quantitative protocol for DNA metabarcoding of springtails (Collembola) (2016)
  • Author(s): Saitoh, S., Aoyama, H., Fujii, S., Sunagawa, H., Nagahama, H., Akutsu, M., Shinzato, N., Kaneko, N., Nakamori, T.
  • Journal Title: Genome Volume: 59 Issue: 9 Pages: 705-723
  • DOI: 10.1139/gen-2015-0228
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Comparison of sampling methods for Collembola on a cobble-dominated riverbank (2016)
  • Author(s): Kawaue, T., Nakamori, T., Iwasaki, Y., Potapov, M.
  • Journal Title: Edaphologia Volume: 98 Pages: 21-27
  • NAID: 110010051129
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] A rapid method of non-destructive DNA extraction from individual springtails (Collembola) (2015)
  • Author(s): Aoyama, H., Saitoh, S., Fujii, S., Nagahama, H., Shinzato, N., Kaneko, N., Nakamori, T.
  • Journal Title: Applied Entomology and Zoology Volume: 未定 Issue: 3 Pages: 419-425
  • DOI: 10.1007/s13355-015-0340-0
  • NAID: 40020571235
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] 日本産ミズトビムシ科およびムラサキトビムシ科（六脚亜門：内顎綱：トビムシ目）の分類 (2014)
  • Author(s): 中森泰三，一澤圭，田村浩志
  • Journal Title: Edaphologia Volume: 95 Pages: 43-82
  • NAID: 110009872650
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] Colorful fungus-Collembola interactions in brown ecosystems (2016)
  • Author(s): Nakamori, T.
  • Organizer: ICSZ-ICA Joint Symposium--Soil biodiversity for our future earth
  • Place of Presentation: Nara Kasugano International Forum, Nara, Japan
  • Int'l Joint Research / Invited
• [Presentation] Reinforcement and adaptation to insecticidal mushroom in Collembola (2016)
  • Author(s): Nakamori, T.
  • Organizer: XIV International Colloquium on Apterygota
  • Place of Presentation: Nara Kasugano International Forum, Nara, Japan
  • Int'l Joint Research
• [Presentation] Is a Lobella species (Collembola: Neanuridae) endemic to Lake Biwa? (2016)
  • Author(s): Nakamori, T., Sawada, K., Tanaka, S., Saitoh, S., Aoyama, H., Fujii, S.
  • Organizer: XIV International Colloquium on Apterygota
  • Place of Presentation: Nara Kasugano International Forum, Nara, Japan
  • Int'l Joint Research
• [Presentation] Multiple mitochondrial sequence types from single collembolan specimens: pseudogenes in Collembola? (2016)
  • Author(s): Saitoh, S., Aoyama, H., Fujii, S., Sanghwa, P., Yamada, A., Shinzato, N., Kaneko, N., Nakamori, T.
  • Organizer: XIV International Colloquium on Apterygota
  • Place of Presentation: Nara Kasugano International Forum, Nara, Japan
  • Int'l Joint Research
• [Presentation] Morphological details and molecular data of Homidia nigrocephala Uchida, 1943 (Collembola: Entomobryidae) from Japan (2016)
  • Author(s): Ichisawa, K., Nakamori, T.
  • Organizer: XIV International Colloquium on Apterygota
  • Place of Presentation: Nara Kasugano International Forum, Nara, Japan
  • Int'l Joint Research
• [Presentation] Lethal effects of Strobilurus fungi on collembolans (2016)
  • Author(s): Kamata, K., Nakamori, T., Pham, D.H., Yoshie, T.
  • Organizer: XIV International Colloquium on Apterygota
  • Place of Presentation: Nara Kasugano International Forum, Nara, Japan
  • Int'l Joint Research / Invited
• [Presentation] Preference of Morulina alata (Collembola: Neanuridae) for fungal sporocarps and effects of gut passage on spores (2016)
  • Author(s): Nakano, M., Tanaka, A., Kamata, K., Nakamori, T.
  • Organizer: XIV International Colloquium on Apterygota, Nara
  • Place of Presentation: Nara Kasugano International Forum, Nara, Japan
  • Int'l Joint Research
• [Presentation] Network architecture of macrofungus-fungivorous Collembola interactions in a Larix kaempferi forest in Japan (2016)
  • Author(s): Nakamori, T., Tanaka, A.
  • Organizer: XVII International Colloquium on Soil Zoology
  • Place of Presentation: Nara Kasugano International Forum, Nara, Japan
  • Int'l Joint Research
• [Presentation] DNA metabarcoding of springtails (Collembola) (2015)
  • Author(s): Saitoh, S. Aoyama, H., Fujii, S., Sunagawa, H., Nagahama, H., Akutsu, M., Shinzato, N., Kaneko, N., & Nakamori, T.
  • Organizer: 6th International Barcode of Life Conference
  • Place of Presentation: University of Guelph, Guelph
  • Year and Date: 2015-08-18
  • Int'l Joint Research
• [Presentation] 沖縄のきのこから得られたトビムシ類の分類学的および生態学的特徴 (2015)
  • Author(s): 中森泰三，Nguyen Duc Hoang Pham，寺嶋芳江，青山洋昭，齋藤星耕，藤井佐織，金子信博
  • Organizer: 日本土壌動物学会第38回大会
  • Place of Presentation: 香川
  • Year and Date: 2015-05-23
• [Presentation] トビムシ類のDNAバーコーディングとメタバーコーディング手法の開発 (2015)
  • Author(s): 齋藤星耕，青山洋昭，藤井佐織，砂川春樹，長濱秀樹，阿久津雅子，新里尚也，金子信博，中森泰三
  • Organizer: 日本土壌動物学会第38回大会
  • Place of Presentation: 香川
  • Year and Date: 2015-05-23
• [Presentation] カラマツ林における大型菌類と菌食トビムシ類の相互作用ネットワークの構造 (2015)
  • Author(s): 中森泰三，田中葵
  • Organizer: 日本菌学会第59回大会
  • Place of Presentation: 那覇
  • Year and Date: 2015-05-16
• [Presentation] 第2世代DNAシーケンサーによる土壌小型節足動物群集の解析手法の開発 (2014)
  • Author(s): 青山洋昭，齋藤星耕，藤井佐織，砂川春樹，長濱秀樹，阿久津雅子，新里尚也，金子信博，中森泰三
  • Organizer: 日本土壌動物学会第37回大会
  • Place of Presentation: 飯能
  • Year and Date: 2014-05-25
• [Presentation] 次世代シーケンサーによる土壌小型節足動物の群集解析手法の開発
  • Author(s): 齋藤星耕, 青山洋昭, 藤井佐織, 砂川春樹, 長濱秀樹, 阿久津雅子, 新里尚也, 松井徹
  • Organizer: 日本土壌壌動学会第36回大会
  • Place of Presentation: 福岡
• [Presentation] 毒きのこは香りで動物に警告する？
  • Author(s): 中森泰三
  • Organizer: 鳥取大学ポストグローバルCOEプログラム「持続性社会構築に向けた菌類きのこ資源活用」主催公開シンポジウム「きのこの香りを科学する―菌類の揮発性物質の機能、役割とその利用」
  • Place of Presentation: 東京
  • Invited
• [Book] 東海大学出版 (2015)
  • Author(s): 青木淳一（編著）
  • Total Pages: 2022
  • Publisher: 日本産土壌動物 第二版: 分類のための図解検索