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The development of systems for evaluation of biodiversity and functioning of soil animals based on next-gereration sequencing analysis

Research Project

Project/Area Number 25281029
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Environmental impact assessment
Research InstitutionYokohama National University

Principal Investigator

Nakamori Taizo  横浜国立大学, 大学院環境情報研究院, 准教授 (50443081)

Co-Investigator(Kenkyū-buntansha) 齋藤 星耕  琉球大学, 熱帯生物圏研究センター, 研究員 (10623754)
青山 洋昭  琉球大学, 熱帯生物圏研究センター, 研究員 (20468078)
金子 信博  横浜国立大学, 環境情報研究科(研究院), 教授 (30183271)
藤井 佐織  横浜国立大学, 環境情報研究院, 産学連携研究員 (50648045)
Project Period (FY) 2013-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
Fiscal Year 2016: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2015: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2013: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
KeywordsDNAバーコード / 生態系影響評価 / 次世代シーケンス / 土壌動物 / 環境評価
Outline of Final Research Achievements

This project contributed to the development of methods for evaluating species, phylogenetic, and functional diversities from community samples of Collembola, a major group of soil arthoropods, based on next-generation sequencing. DNA barcoding was performed for about 100 species and their phylogenetic tree was estimated. Trait data were obtained by literature surveys and laboratory and field observations for some species. A quantitative protocol for community analysis using next-generation sequencing was provided.

Report

(5 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • Research Products

    (23 results)

All 2016 2015 2014 Other

All Int'l Joint Research (3 results) Journal Article (4 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 4 results,  Open Access: 1 results,  Acknowledgement Compliant: 4 results) Presentation (15 results) (of which Int'l Joint Research: 9 results,  Invited: 3 results) Book (1 results)

  • [Int'l Joint Research] Biotechnology Center of Hochiminh City(ベトナム)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] Moscow State Pedagogical University(ロシア連邦)

    • Related Report
      2015 Annual Research Report
  • [Int'l Joint Research] Biotechnology Center of Hochiminh City(ベトナム)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] A quantitative protocol for DNA metabarcoding of springtails (Collembola)2016

    • Author(s)
      Saitoh, S., Aoyama, H., Fujii, S., Sunagawa, H., Nagahama, H., Akutsu, M., Shinzato, N., Kaneko, N., Nakamori, T.
    • Journal Title

      Genome

      Volume: 59 Issue: 9 Pages: 705-723

    • DOI

      10.1139/gen-2015-0228

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Comparison of sampling methods for Collembola on a cobble-dominated riverbank2016

    • Author(s)
      Kawaue, T., Nakamori, T., Iwasaki, Y., Potapov, M.
    • Journal Title

      Edaphologia

      Volume: 98 Pages: 21-27

    • NAID

      110010051129

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] A rapid method of non-destructive DNA extraction from individual springtails (Collembola)2015

    • Author(s)
      Aoyama, H., Saitoh, S., Fujii, S., Nagahama, H., Shinzato, N., Kaneko, N., Nakamori, T.
    • Journal Title

      Applied Entomology and Zoology

      Volume: 未定 Issue: 3 Pages: 419-425

    • DOI

      10.1007/s13355-015-0340-0

    • NAID

      40020571235

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] 日本産ミズトビムシ科およびムラサキトビムシ科(六脚亜門:内顎綱:トビムシ目)の分類2014

    • Author(s)
      中森泰三,一澤圭,田村浩志
    • Journal Title

      Edaphologia

      Volume: 95 Pages: 43-82

    • NAID

      110009872650

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] Colorful fungus-Collembola interactions in brown ecosystems2016

    • Author(s)
      Nakamori, T.
    • Organizer
      ICSZ-ICA Joint Symposium--Soil biodiversity for our future earth
    • Place of Presentation
      Nara Kasugano International Forum, Nara, Japan
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Reinforcement and adaptation to insecticidal mushroom in Collembola2016

    • Author(s)
      Nakamori, T.
    • Organizer
      XIV International Colloquium on Apterygota
    • Place of Presentation
      Nara Kasugano International Forum, Nara, Japan
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Is a Lobella species (Collembola: Neanuridae) endemic to Lake Biwa?2016

    • Author(s)
      Nakamori, T., Sawada, K., Tanaka, S., Saitoh, S., Aoyama, H., Fujii, S.
    • Organizer
      XIV International Colloquium on Apterygota
    • Place of Presentation
      Nara Kasugano International Forum, Nara, Japan
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Multiple mitochondrial sequence types from single collembolan specimens: pseudogenes in Collembola?2016

    • Author(s)
      Saitoh, S., Aoyama, H., Fujii, S., Sanghwa, P., Yamada, A., Shinzato, N., Kaneko, N., Nakamori, T.
    • Organizer
      XIV International Colloquium on Apterygota
    • Place of Presentation
      Nara Kasugano International Forum, Nara, Japan
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Morphological details and molecular data of Homidia nigrocephala Uchida, 1943 (Collembola: Entomobryidae) from Japan2016

    • Author(s)
      Ichisawa, K., Nakamori, T.
    • Organizer
      XIV International Colloquium on Apterygota
    • Place of Presentation
      Nara Kasugano International Forum, Nara, Japan
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Lethal effects of Strobilurus fungi on collembolans2016

    • Author(s)
      Kamata, K., Nakamori, T., Pham, D.H., Yoshie, T.
    • Organizer
      XIV International Colloquium on Apterygota
    • Place of Presentation
      Nara Kasugano International Forum, Nara, Japan
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Preference of Morulina alata (Collembola: Neanuridae) for fungal sporocarps and effects of gut passage on spores2016

    • Author(s)
      Nakano, M., Tanaka, A., Kamata, K., Nakamori, T.
    • Organizer
      XIV International Colloquium on Apterygota, Nara
    • Place of Presentation
      Nara Kasugano International Forum, Nara, Japan
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Network architecture of macrofungus-fungivorous Collembola interactions in a Larix kaempferi forest in Japan2016

    • Author(s)
      Nakamori, T., Tanaka, A.
    • Organizer
      XVII International Colloquium on Soil Zoology
    • Place of Presentation
      Nara Kasugano International Forum, Nara, Japan
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] DNA metabarcoding of springtails (Collembola)2015

    • Author(s)
      Saitoh, S. Aoyama, H., Fujii, S., Sunagawa, H., Nagahama, H., Akutsu, M., Shinzato, N., Kaneko, N., & Nakamori, T.
    • Organizer
      6th International Barcode of Life Conference
    • Place of Presentation
      University of Guelph, Guelph
    • Year and Date
      2015-08-18
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 沖縄のきのこから得られたトビムシ類の分類学的および生態学的特徴2015

    • Author(s)
      中森泰三,Nguyen Duc Hoang Pham,寺嶋芳江,青山洋昭,齋藤星耕,藤井佐織,金子信博
    • Organizer
      日本土壌動物学会第38回大会
    • Place of Presentation
      香川
    • Year and Date
      2015-05-23
    • Related Report
      2015 Annual Research Report
  • [Presentation] トビムシ類のDNAバーコーディングとメタバーコーディング手法の開発2015

    • Author(s)
      齋藤星耕,青山洋昭,藤井佐織,砂川春樹,長濱秀樹,阿久津雅子,新里尚也,金子信博,中森泰三
    • Organizer
      日本土壌動物学会第38回大会
    • Place of Presentation
      香川
    • Year and Date
      2015-05-23
    • Related Report
      2015 Annual Research Report
  • [Presentation] カラマツ林における大型菌類と菌食トビムシ類の相互作用ネットワークの構造2015

    • Author(s)
      中森泰三,田中葵
    • Organizer
      日本菌学会第59回大会
    • Place of Presentation
      那覇
    • Year and Date
      2015-05-16
    • Related Report
      2015 Annual Research Report
  • [Presentation] 第2世代DNAシーケンサーによる土壌小型節足動物群集の解析手法の開発2014

    • Author(s)
      青山洋昭,齋藤星耕,藤井佐織,砂川春樹,長濱秀樹,阿久津雅子,新里尚也,金子信博,中森泰三
    • Organizer
      日本土壌動物学会第37回大会
    • Place of Presentation
      飯能
    • Year and Date
      2014-05-25
    • Related Report
      2014 Annual Research Report
  • [Presentation] 次世代シーケンサーによる土壌小型節足動物の群集解析手法の開発

    • Author(s)
      齋藤星耕, 青山洋昭, 藤井佐織, 砂川春樹, 長濱秀樹, 阿久津雅子, 新里尚也, 松井徹
    • Organizer
      日本土壌壌動学会第36回大会
    • Place of Presentation
      福岡
    • Related Report
      2013 Annual Research Report
  • [Presentation] 毒きのこは香りで動物に警告する?

    • Author(s)
      中森泰三
    • Organizer
      鳥取大学ポストグローバルCOEプログラム「持続性社会構築に向けた菌類きのこ資源活用」主催公開シンポジウム「きのこの香りを科学する―菌類の揮発性物質の機能、役割とその利用」
    • Place of Presentation
      東京
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Book] 東海大学出版2015

    • Author(s)
      青木淳一(編著)
    • Total Pages
      2022
    • Publisher
      日本産土壌動物 第二版: 分類のための図解検索
    • Related Report
      2014 Annual Research Report

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Published: 2013-05-21   Modified: 2022-01-31  

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