| Budget Amount *help |
¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000)
Fiscal Year 2015: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥11,180,000 (Direct Cost: ¥8,600,000、Indirect Cost: ¥2,580,000)
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| Outline of Final Research Achievements |
Among various mechanisms of anaerobic benzene degradation, no isolates has been obtained for the degradation under methanogenic condition, and thus little is known about the degradation mechanisms. Putative benzene degrading bacterium Hasda-A, which we identified before, has been enriched by electrochemical cultivation. Single cell of Hasda-A was obtained by cell sorting and the genome sequence was analyzed. We found Benzoyl Co-A reductase gene, which is in an important pathway of anaerobic benzene degradation. Previously known initial benzene degradation metabolism, neither calboxylase gene in iron reducing condition nor gene for methylation known in nitrate reducing condition, was found. It implied the novel initial metabolism for the methanogenic enrichment culture.
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