Functional Analysis of Anaerobic Benzene Degrading Microbial Community by Single Cell Genomics
| Project/Area Number |
25281043
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Modeling and technologies for environmental conservation and remediation
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| Research Institution | The University of Tokyo |
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Principal Investigator |
Kurisu Futoshi 東京大学, 工学(系)研究科(研究院), 准教授 (30312979)
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| Co-Investigator(Kenkyū-buntansha) |
KASUGA Ikuro 東京大学, 大学院工学系研究科, 講師 (20431794)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000)
Fiscal Year 2015: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥11,180,000 (Direct Cost: ¥8,600,000、Indirect Cost: ¥2,580,000)
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| Keywords | バイオレメディエーション / ベンゼン / シングルセルゲノム / 電気培養 / ベンゼン分解 |
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| Outline of Final Research Achievements |
Among various mechanisms of anaerobic benzene degradation, no isolates has been obtained for the degradation under methanogenic condition, and thus little is known about the degradation mechanisms. Putative benzene degrading bacterium Hasda-A, which we identified before, has been enriched by electrochemical cultivation. Single cell of Hasda-A was obtained by cell sorting and the genome sequence was analyzed. We found Benzoyl Co-A reductase gene, which is in an important pathway of anaerobic benzene degradation. Previously known initial benzene degradation metabolism, neither calboxylase gene in iron reducing condition nor gene for methylation known in nitrate reducing condition, was found. It implied the novel initial metabolism for the methanogenic enrichment culture.
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Report
(4 results)
Research Products
(8 results)
