Molecular basis of metabolic physiology of C1-microorganisms and its application in methanol economy

• Project/Area Number: 25281063
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Design and evaluation of sustainable and environmental conscious system
• Research Institution: Kyoto University
• Principal Investigator: YURIMOTO Hiroya 京都大学, (連合)農学研究科(研究院), 准教授 (00283648)
• Co-Investigator(Renkei-kenkyūsha): SAKAI Yashuyoshi 京都大学, 大学院農学研究科, 教授 (60202082) ; OKU Masahide 京都大学, 大学院農学研究科, 助教 (10511230)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000); Fiscal Year 2015: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2014: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2013: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
• Keywords: メタノール資化性酵母 / 転写因子 / C1化合物代謝 / メタン / メタノール / ホルムアルデヒド / 硝酸 / メチルアミン

Outline of Final Research Achievements

For the purpose of development of novel biotechnology that can be applied in low carbon society “methanol economy”, we have studied molecular basis of metabolic physiology of C1-microorganisms that can utilize C1 compounds, such as methane and methanol, and its application. We focused on two major useful traits of methylotrophic yeasts, “strong methanol-inducible gene expression” and “ability to proliferate at the phyllosphere”. For example, molecular function of the transcriptional factor complex responsible for methanol-inducible gene expression was elucidated. Furthermore, we revealed nitrogen utilization and its regulation of the methylotrophic yeast at the phyllosphere.

Research Products

• [Journal Article] The unique C-terminal region of Hap3 is required for methanol-regulated gene expression in the methylotrophic yeast Candida boidinii (2016)
  • Author(s): Saori Oda, Hiroya Yurimoto, Nobuhisa Nitta, and Yasuyoshi Sakai
  • Journal Title: Microbiology Volume: 印刷中 Issue: 5 Pages: 898-907
  • DOI: 10.1099/mic.0.000275
  • NAID: 120007181191
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Draft genomes of gammaproteobacterial methanotrophs isolated from terrestrial ecosystems (2015)
  • Author(s): Richard Hamilton, K. Dimitri Kits, Victoria A. Ramonovskaya, Olga N. Rozova, Hiroya Yurimoto, Hiroyuki Iguchi, Valentina N. Khmelenina, Yasuyoshi Sakai, et al.
  • Journal Title: Genome Announc. Volume: 3 Issue: 3
  • DOI: 10.1128/genomea.00515-15
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Regulation of nitrate and methylamine metabolism by multiple nitrogen sources in the methylotrophic yeast Candida boidinii (2015)
  • Author(s): Kosuke Shiraishi, Masahide Oku, Daichi Uchida, Hiroya Yurimoto, Yasuyoshi Sakai
  • Journal Title: FEMS Yeast Research Volume: 15 Pages: fov084-fov084
  • DOI: 10.1093/femsyr/fov084
  • Peer Reviewed
• [Journal Article] C1微生物-植物共生系によるC1炭素固定と植物生長促進 (2015)
  • Author(s): 由里本博也、阪井康能
  • Journal Title: 光合成研究 Volume: 25 Pages: 92-99
  • NAID: 40020570695
  • Peer Reviewed
• [Journal Article] C1微生物の巧妙なストレス適応機構と多様な生育環境での生存戦略 (2015)
  • Author(s): 由里本博也、阪井康能
  • Journal Title: 極限環境生物学会誌 Volume: 14 Pages: 52-60
  • NAID: 40020753808
  • Peer Reviewed
• [Journal Article] Molecular characterization of Hap complex components responsible for methanol-inducible gene expression in the methylotrophic yeast Candida boidinii (2015)
  • Author(s): S. Oda, H. Yurimoto, N. Nitta, Y. Sasano, and Y. Sakai
  • Journal Title: Eukaryot. Cell Volume: 14 Issue: 3 Pages: 278-285
  • DOI: 10.1128/ec.00285-14
  • NAID: 120005593631
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Interactions of methylotrophs with plants and other hHeterotrophic bacteria (2015)
  • Author(s): H. Iguchi, H. Yurimoto, and Y. Sakai
  • Journal Title: Microorganisms Volume: 3 Issue: 2 Pages: 137-151
  • DOI: 10.3390/microorganisms3020137
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Yeast nitrogen utilization in the phyllosphere during plant lifespan under regulation of autophagy (2015)
  • Author(s): K. Shiraishi, M. Oku, K. Kawaguchi, D. Uchida, H. Yurimoto, and Y. Sakai
  • Journal Title: Sci. Rep. Volume: 5 Issue: 1 Pages: 9719-9719
  • DOI: 10.1038/srep09719
  • NAID: 120005615631
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Methyloparacoccus murrellii gen. nov., sp. nov., a methanotroph isolated from pond water (2014)
  • Author(s): S. Hoefman, D. van der Ha, H. Iguchi, H. Yurimoto, Y. Sakai, N. Boon, P. Vandamme, K. Heylen, and P. de Vos
  • Journal Title: Int. J. Syst. Evol. Microbiol. Volume: 64 Issue: Pt_6 Pages: 2100-2107
  • DOI: 10.1099/ijs.0.057760-0
  • Peer Reviewed
• [Journal Article] Xylitol Production by Thermotolerant Methylotrophic Yeast Ogataea siamensis and Its Xylose Reductase Gene (XYL1) Cloning (2014)
  • Author(s): W. Boontham, N. Srisuk, K. Kokaew, P. Treeyoung, S. Limtong, A. Thamchaipenet、 and H. Yurimoto
  • Journal Title: Chiang Mai J. Sci. Volume: 41 Pages: 491-502
  • Peer Reviewed / Open Access
• [Journal Article] Aquatic plant surface as a niche for methanotrophs. (2014)
  • Author(s): Yoshida N, Iguchi H, Yurimoto H, Murakami A, Sakai Y
  • Journal Title: Frontiers in Microbiology / Terrestrial Microbiology Volume: 5 Pages: 1-8
  • DOI: 10.3389/fmicb.2014.00030
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] 植物から放出されるC1化合物と微生物―植物間相互作用による炭素循環 (2014)
  • Author(s): 由里本博也、阪井康能
  • Journal Title: AROMA RESEARCH Volume: 15 Pages: 80-84
• [Journal Article] Expression of a codon-optimized Aspergillus niger pectin methylesterase gene in the methylotrophic yeast Candida boidinii. (2014)
  • Author(s): K. Kawaguchi, H. Yurimoto, and Y. Sakai
  • Journal Title: Biosci. Biotechnol. Biochem. Volume: 78
  • Peer Reviewed
• [Journal Article] Dominant Colonization and Inheritance of <i>Methylobacterium</i> sp. Strain OR01 on Perilla Plants (2013)
  • Author(s): Mizuno M, Yurimoto H, Iguchi H, Tani A, Sakai Y
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 77 Issue: 7 Pages: 1533-1538
  • DOI: 10.1271/bbb.130207
  • NAID: 10031190465
  • ISSN: 0916-8451, 1347-6947
  • Peer Reviewed
• [Journal Article] C1微生物代謝経路の省エネ型炭素固定系としての利用とその問題点 (2013)
  • Author(s): 由里本博也、阪井康能
  • Journal Title: 生物工学会誌 Volume: 91 Pages: 384-387
• [Journal Article] C1微生物複合生物系が駆動する炭素循環（メタンサイクル） (2013)
  • Author(s): 由里本博也、井口博之、阪井康能
  • Journal Title: 生物の科学 遺伝 Volume: 67 Pages: 591-595
• [Journal Article] Stress resistance and C1 metabolism involved in plant colonization of a methanotroph Methylosinus sp. B4S. (2013)
  • Author(s): H. Iguchi, I. Sato, H. Yurimoto, and Y. Sakai.
  • Journal Title: Arch. Microbiol. Volume: 195 Issue: 10-11 Pages: 2161-2162
  • DOI: 10.1007/s00203-013-0922-6
  • NAID: 120005526706
  • Peer Reviewed
• [Journal Article] 葉面に棲息するC1微生物：共生系による炭素循環と環境バイオへの利用 (2013)
  • Author(s): 阪井康能、由里本博也
  • Journal Title: 環境バイオテクノロジー学会誌 Volume: 13 Pages: 79-84
• [Presentation] Studies on C1-microorganisms and their applications in biotechnology (2015)
  • Author(s): Hiroya Yurimoto
  • Organizer: The 1st KU-KUGSA Bilateral Symposium on “Food, Environment and Life for the Next Generation”
  • Place of Presentation: バンコク（タイ）
  • Year and Date: 2015-12-16
  • Int'l Joint Research / Invited
• [Presentation] Symbiotic interaction between C1-microorganisms and plants and its applications (2015)
  • Author(s): Hiroya Yurimoto
  • Organizer: International Symposium on Microbial Research and Biotechnology for Biomass Utilization
  • Place of Presentation: JR博多シティ（福岡県・福岡市）
  • Year and Date: 2015-11-12
  • Int'l Joint Research / Invited
• [Presentation] Transcription factors involved in regulation of methanol-inducible gene expression in the methylotrophic yeast (2015)
  • Author(s): Hiroya Yurimoto
  • Organizer: 32nd International Specialized Symposium on Yeasts (ISSY32)
  • Place of Presentation: ペルージャ（イタリア）
  • Year and Date: 2015-09-16
  • Int'l Joint Research / Invited
• [Presentation] C1微生物の巧妙なストレス適応機構と多様な生育環境での生存戦略 (2015)
  • Author(s): 由里本博也
  • Organizer: 極限環境生物学会第16回シンポジウム「極限環境生物が秘めるストレス適応能力」
  • Place of Presentation: 京都大学（京都府・京都市）
  • Year and Date: 2015-06-06
  • Invited
• [Presentation] 植物から放出されるC1化合物と葉面微生物-植物間相互作用によるC1炭素循環 (2014)
  • Author(s): 由里本博也、阪井康能
  • Organizer: 第4回生物起源微量ガスワークショップ
  • Place of Presentation: 文部科学省研究交流センター（茨城県・つくば市）
  • Year and Date: 2014-11-20 – 2014-11-21
  • Invited
• [Presentation] Metabolic alteration by introducing bifunctional formaldehyde-fixing enzyme into the methylotrophic bacteria (2014)
  • Author(s): H. Yurimoto, D. Kajihara, and Y. Sakai
  • Organizer: Gordon Research Conference on Molecular basis of microbial one-carbon metabolism
  • Place of Presentation: South Hadley, MA（米国）
  • Year and Date: 2014-08-10 – 2014-08-15
• [Presentation] Molecular characterization of Hap complex components responsible for methanol-inducible gene expression in the methylotrophic yeast Candida boidinii (2014)
  • Author(s): S. Oda, H. Yurimoto, and Y. Sakai
  • Organizer: Gordon Research Conference on Molecular basis of microbial one-carbon metabolism
  • Place of Presentation: South Hadley, MA（米国）
  • Year and Date: 2014-08-10 – 2014-08-15
• [Presentation] 微生物ホルムアルデヒド代謝の戦略的活用 (2014)
  • Author(s): 由里本博也、阪井康能
  • Organizer: 日本農芸化学会2014年度大会シンポジウム 「アルデヒドのバイオサイエンス ―生物の多様なアルデヒド代謝系とその戦略的活用―」
  • Place of Presentation: 明治大学（川崎市）
  • Invited
• [Presentation] Metabolic alteration by introducing bifunctional formaldehyde-fixing enzyme into the methylotrophic bacterium (2013)
  • Author(s): H. Yurimoto, D. Kajihara, and Y. Sakai
  • Organizer: V International Conference on Environmental, Industrial and Applied Microbiology
  • Place of Presentation: マドリッド（スペイン）
  • Invited
• [Presentation] 植物から放出されるC1化合物と微生物－植物間相互作用による炭素循環
  • Author(s): 由里本博也、阪井康能
  • Organizer: 第252回生存圏シンポジウム 「植物アロマのメタ代謝科学 ～生態学、大気科学、植物科学の融合～」
  • Place of Presentation: 京都大学（宇治市）
  • Invited
• [Book] オルガネラタンパク質の輸送・分解の分子基盤と有用タンパク質生産 －メタノール資化性酵母の発達したペルオキシソームの利用 「酵母の生命科学と生物工学 産業応用から基礎科学へ」 (2013)
  • Author(s): 阪井康能、由里本博也、奥公秀
  • Publisher: 化学同人