Study on reduction of the risk for foodborne illness by fresh produce

• Project/Area Number: 25282019
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Eating habits
• Research Institution: Kyushu University
• Principal Investigator: Miyamoto Takahisa 九州大学, (連合)農学研究科(研究院), 教授 (70190816)
• Co-Investigator(Kenkyū-buntansha): HONJOH KEN-ICHI 九州大学, 大学院農学研究院, 准教授 (00264101)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥19,240,000 (Direct Cost: ¥14,800,000、Indirect Cost: ¥4,440,000); Fiscal Year 2015: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2014: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2013: ¥10,400,000 (Direct Cost: ¥8,000,000、Indirect Cost: ¥2,400,000)
• Keywords: サルモネラ / 腸管出血性大腸菌 / リステリア / 汚染機構 / 生残性 / 野菜 / 二次付着 / 生産段階 / レタス / 付着力 / 阻害 / ポリリジン / 乳清タンパク質 / ほうれん草 / 生残機構 / トマト / 表面付着 / マルチ

Outline of Final Research Achievements

This study was conducted to clarify the mechanism for contamination of vegetables by foodborne pathogens during cultivation. Salmonella Enteritidis, Escherichia coli O157 and Listeria monocytogenes survived until harvest time for 70d depending on the initial inoculum. After cultivation on these contaminated soil, tomato fruits were not contaminated with the pathogens, however, lettuce and spinach were contaminated with the pathogens depending on the level of pathogen contamination of soil. The pathogens inoculated on the surface of these vegetables disappeared in a few days, though they survived much longer on the injured surfaces. Pretreatment of the surface of vegetables with polylysine and milk serum protein decreased the secondary attachment of the pathogens onto the vegetables.

Research Products

• [Journal Article] Investigation of Routes of <i>Salmonella</i> Contamination Via Soils and the Use of Mulch for Contamination Control during Lettuce Cultivation (2014)
  • Author(s): Honjoh, K., Tomoko Mishima, Nozomi Kido, Misako Shimamoto, Miyamoto, T
  • Journal Title: Food Science and Technology Research Volume: 20 Issue: 5 Pages: 961-969
  • DOI: 10.3136/fstr.20.961
  • NAID: 130004704875
  • ISSN: 1344-6606, 1881-3984
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] 栽培土壌におけるリステリアの損傷ならびにリーフレタス可食部汚染機構の解明 (2015)
  • Author(s): 本城 賢一, 林 英, 祝迫侑里, 城 聖美, 木嶋伸行, 宮本 敬久,
  • Organizer: 第36回日本食品微生物学会学術総会
  • Place of Presentation: 川崎市
  • Year and Date: 2015-11-12
• [Presentation] 農産物の食中毒細菌汚染低減法の開発 (2015)
  • Author(s): 森永憲子, 小椋彩, Md Tariqul Islam, 本城 賢一, 宮本 敬久
  • Organizer: 平成27年度（公社）日本食品科学工学会西日本支部 および（公社）日本栄養・食糧学会九州・沖縄支部合同大会
  • Place of Presentation: 那覇市
  • Year and Date: 2015-10-31
• [Presentation] CONTAMINATION AND SURVIVAL OF SALMONELLA ON LEAF VEGETABLE DURING CULTIVATION (2014)
  • Author(s): Takahisa Miyamoto, Misako Shimamoto, Nozomi Kido, Tomoko Mishima, Ken-ichi Honjoh,
  • Organizer: The 8th International Symposium on the East Asian Environmental Problems (EAEP2014)
  • Place of Presentation: 福岡市
  • Year and Date: 2014-12-08 – 2014-12-10
• [Presentation] リステリア汚染土壌で栽培したトマトの可食部汚染の可能性について, (2014)
  • Author(s): 祝迫 侑里, 林 英, 本城 賢一, 宮本 敬久,
  • Organizer: 平成26年度 日本食品科学工学会西日本支部大会
  • Place of Presentation: 福岡市
  • Year and Date: 2014-12-06
• [Presentation] Studies on contamination routes of Salmonella Enteritidis and Listeria monocytogenes in vegetables (2014)
  • Author(s): Ken-ichi Honjoh, Tomoko Mishima, Nozomi Kido, Yuri Iwaizako, Ying Lin, Takahisa Miyamoto
  • Organizer: 43rd Annual Meeting United States - Japan Cooperative Program in Natural Resources Food and Agricultural Panel
  • Place of Presentation: アトランタ、米国
  • Year and Date: 2014-10-20 – 2014-10-23
• [Presentation] トマトにおけるサルモネラの付着，生残機構の解明 (2013)
  • Author(s): 米田 朋樹, 三島 朋子, 城戸 希望, 本城 賢一, 宮本 敬久
  • Organizer: 日本食品科学工学会第60回記念大会
  • Place of Presentation: 東京都日野市
• [Remarks] 九州大学食品衛生化学研究室 研究内容
  • URL: http://www.agr.kyushu-u.ac.jp/lab/foodhygienicchemistry/page03.html