Establishment of ESR imaging methods for evaluation of skin diseases
| Project/Area Number |
25282124
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biomaterial science and engineering
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| Research Institution | Hirosaki University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
SAWAMURA DAISUKE 弘前大学, 大学院医学研究科, 教授 (60196334)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥15,600,000 (Direct Cost: ¥12,000,000、Indirect Cost: ¥3,600,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2014: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2013: ¥10,660,000 (Direct Cost: ¥8,200,000、Indirect Cost: ¥2,460,000)
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| Keywords | 電子スピン共鳴 / フリーラジカル / 生体計測 / イメージング / 皮膚科学 / 皮膚疾患 / 生体情報 / ESR / 皮膚構造 / スピンプローブ |
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| Outline of Final Research Achievements |
We investigated identification and locations of abnormality of the stratum corneum (SC) in patients with psoriasis vulgaris (PV) by using 9 GHz electron spin resonance (ESR) imaging. The pieces of the SC and PV-SC were incubated in an aqueous solution of a spin probe for about 60 minutes at 37 oC. The probe solution was dropped on the sample sheet. The SC sheet repels the aqueous solution but the probe goes into the lipid phase during the incubation. After rinsing with distilled water to remove excess spin probe, the sample was mounted on an ESR cell and was measured by the ESR spectrometer. All measurements were performed at room temperature.
The strong red signal is due to probe penetration into the PV skin. No red lesion region was observed in the control. The two-dimensionalimages showed various sizes and number distribution concerning the disordered states in the SC structure. Thus, 9 GHz ESR imaging can be useful for identifying the location of abnormality of the SC states.
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Report
(4 results)
Research Products
(65 results)
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[Presentation] X-band ESR imaging for foodstuffs2015
Author(s)
Hideyuki Hara, Kouichi Nakagawa
Organizer
The 3rd Awaji International Workshop on Electron Spin Science and Technology: Biological and Material Science oriented Application (3rd AWEST 2015)
Place of Presentation
Awaji Island (兵庫県、淡路市)
Year and Date
2015-06-15
Related Report
Int'l Joint Research
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[Presentation] EPR Spin Probe Investigation of Skin Structure2014
Author(s)
Kouichi Nakagawa, Satoko Minakawa, and Daisuke Sawamura
Organizer
The 2nd Awaji International Workshop on Electron Spin Science and Technology: Biological and Material Science oriented Application (2nd AWEST 2014)
Place of Presentation
Awaji Yumebutai International Conference Center, Awaji Island, Hyogo, Japan
Year and Date
2014-06-15 – 2014-06-17
Related Report
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[Presentation] 角層細胞間脂質モデルの構造と特性2014
Author(s)
箱田優也, 田中佳祐, 油井研一, 酒井健一, 酒井秀樹, 阿部正彦, 橋本悟, 中川公一, 坂本一民
Organizer
第39回日本香粧品学会
Place of Presentation
有楽町朝日ホール（東京都、千代田区）
Year and Date
2014-06-05 – 2014-06-06
Related Report
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[Presentation] 9 GHz EPR Investigation of Skin Surface2013
Author(s)
K. Nakagawa, S. Minakawa, and D. Sawamura
Organizer
The 1st Awaji International Workshop on Electron Spin Science and Technology: Biological and Material Science oriented Application
Place of Presentation
Awaji Island, Hyogo
Related Report
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