| Budget Amount *help |
¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000)
Fiscal Year 2015: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2014: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥9,880,000 (Direct Cost: ¥7,600,000、Indirect Cost: ¥2,280,000)
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| Outline of Final Research Achievements |
Vascular endothelial cells (ECs) respond to the hemodynamic shear stress and stretch by altering their morphology, functions, and gene expression. Here we conclude that plasma membranes differentiate between stretch and shear stress by undergoing transitions in their lipid phases. Shear stress decreased the lipid order of EC plasma membranes; stretching increased the membrane lipid order. Similar changes occurred in the artificial lipid bilayer membranes, indicating that these are physical phenomena. Blocking these changes in the membrane lipid order by depleting membrane cholesterol with MβCD or by adding cholesterol resulted in marked inhibitions of the stretch or shear stress induced phosphorylation of PDGF receptors or VEGF receptors, respectively. These results indicate that EC membranes differently respond to hemodynamic forces and that the changes in membrane physical properties are involved in the mechanotransduction that activates membrane receptors by each force.
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