Development of a stem cell-based self-assembled tissue cultured on a nano-periodic-patterned surface: Fiber reinforcement and improvement of soft tissue repair
Project/Area Number |
25282134
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Partial Multi-year Fund |
Section | 一般 |
Research Field |
Biomedical engineering/Biomaterial science and engineering
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Research Institution | Tokyo Metropolitan University |
Principal Investigator |
FUJIE HIROMICHI 首都大学東京, システムデザイン学部, 教授 (20199300)
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Co-Investigator(Kenkyū-buntansha) |
Nakamura Norimasa 大阪大学, 臨床医工学融合研究教育センター, 招聘教授 (50273719)
|
Project Period (FY) |
2013-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥10,530,000 (Direct Cost: ¥8,100,000、Indirect Cost: ¥2,430,000)
Fiscal Year 2013: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
|
Keywords | バイオメカニクス / 間葉系幹細胞 / 自己生成組織 / ナノ加工 / 軟組織修復 / ナノ線維 / 生体軟組織修復 |
Outline of Final Research Achievements |
Two kinds of stem cell-based self-assembled tissues (SATs) were produced through either a cell culture of mesenchymal stem cell (MSC) on a nano-periodic-structured surface (nano-SAT) or a cell culture of MSC on nano-collagen fibers (fiber-SAT). Both the STAs exhibited more complicated fiber-reinforced structure with stiffer and stronger properties than normal SAT. Then, either of the nano-SAT or the fiber-SAT was implanted into partial chondro-defects of rabbit femoral cartilage. At 3 months after implantation, the repair tissues exhibited well-organized cartilage-like structures with abundant type-II collagen. In particular, tissues repaired with the fiber-SAT exhibited a low coefficient of friction with a small variation. In addition, we found that the fiber-SAT had a significant property for the repair of transected patellar tendon.
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Report
(4 results)
Research Products
(25 results)
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[Journal Article] Stem Cell-Based Self-Assembled Tissues Cultured on a Nano-Periodic-Structured Surface Patterned Using Femtosecond Laser Processing2016
Author(s)
Fujie, H., Oya, K., Tani, Y., Suzuki, K., Nakamura, N.
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Journal Title
International Journal of Automation Technology
Volume: 10
Issue: 1
Pages: 55-61
DOI
NAID
ISSN
1881-7629, 1883-8022
Year and Date
2016-01-05
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Osteochondral repair using a scaffold-free tissue engineered construct derived from synovial MSCs and a hydroxyaptite-based artificial bone2014
Author(s)
Shimomura K, Moriguchi Y, Ando W, Nansai R, Fujie H, Hart David, Gobbi A, Kita K, Horibe S, Shino K, Yoshikawa H, Nakamura N
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Journal Title
Tissue Engineering Part A
Volume: 20
Pages: 2291-2304
Related Report
Peer Reviewed
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