• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Construction of cell culture environment based on regulation of orientation and denaturation of adsorbed proteins by molecular mobility

Research Project

Project/Area Number 25282142
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Biomedical engineering/Biomaterial science and engineering
Research InstitutionTokyo Medical and Dental University

Principal Investigator

Yui Nobuhiko  東京医科歯科大学, 生体材料工学研究所, 教授 (70182665)

Co-Investigator(Kenkyū-buntansha) TAMURA ATSUSHI  東京医科歯科大学, 生体材料工学研究所, 助教 (80631150)
ARISAKA YOSHINORI  東京医科歯科大学, 生体材料工学研究所, 助教 (70590115)
SEO JI-HUN  東京医科歯科大学, 生体材料工学研究所, 助教 (20611544)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000)
Fiscal Year 2015: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2013: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
Keywordsポリロタキサン / 分子運動性 / 細胞機能調節 / ブロック共重合体 / 運動性 / タンパク質吸着 / 和周波発生分光法 / 細胞分化 / RAFT / 超分子
Outline of Final Research Achievements

For constructing biocompatible environments using molecularly mobile surfaces based on polyrotaxane (PRX), we fabricated PRX-coated surfaces and evaluated the effect of the molecular mobility on protein adsorption and the differentiation of stem cells on PRX surfaces. By regulating Rho-family GTPases depending on molecular mobility of PRX surfaces, mesenchymal stem cells (MSCs) on PRX surface with lowly molecular mobility were introduced the differentiation into an osteoblast linage, and those on PRX surfaces with highly mobile surfaces were introduced the differentiation into an adipocyte lineage. In addition, induced pluripotent stem (iPS) cells on PRX surface with highly molecular mobility were introduced the differentiation into a cardiomyocyte lineage. Therefore, molecularly mobile surfaces were useful as new cell culture environments for the modulation of cellular function by regulating the cytoskeleton-related signaling pathway.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • Research Products

    (23 results)

All 2016 2015 2014

All Journal Article (6 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 6 results,  Acknowledgement Compliant: 5 results) Presentation (15 results) (of which Int'l Joint Research: 1 results,  Invited: 8 results) Book (2 results)

  • [Journal Article] Dynamic polyrotaxane-coated surface for effective differentiation of mouse induced pluripotent stem cells into cardiomyocytes2016

    • Author(s)
      Ji-Hun Seo, Mitsuhi Hirata, Sachiro Kakinoki, Tetsuji Yamaoka and Nobuhiko Yui
    • Journal Title

      RSC Advances

      Volume: 6 Issue: 42 Pages: 35668-35676

    • DOI

      10.1039/c6ra03967g

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Structural reorganization and fibrinogen adsorption behaviors on the polyrotaxane surfaces investigated by sum frequency generation spectroscopy2015

    • Author(s)
      Aimin Ge, Ji-Hun Seo, Lin Qiao, Nobuhiko Yui, and Shen Ye
    • Journal Title

      ACS Applied Materials and Interfaces

      Volume: 7 Issue: 40 Pages: 22709-22718

    • DOI

      10.1021/acsami.5b07760

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] UV-Cleavable polyrotaxane cross-linker for modulating mechanical strength of photocurable resin plastics2015

    • Author(s)
      Ji-Hun Seo, Mayu Fushimi, Naoko Matsui, Tomohiro Takagaki, Junji Tagami, and Nobuhiko Yui
    • Journal Title

      ACS Macro Letters

      Volume: 4 Issue: 10 Pages: 1154-1157

    • DOI

      10.1021/acsmacrolett.5b00619

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Modulation of friction dynamics in water by changing the combination of the loop- and grafttype poly(ethylene glycol) surfaces2015

    • Author(s)
      Seo JH, Tsutsumi Y, Kobari A, Shimojo M, Hanawa T, Yui N
    • Journal Title

      Soft Materials

      Volume: 11 Issue: 5 Pages: 936-942

    • DOI

      10.1039/c4sm02082k

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Directing stem cell differentiation by changing the molecular mobility of supramolecular surfaces2015

    • Author(s)
      J.-H. Seo, S. Kakinoki, T. Yamaoka, N. Yui
    • Journal Title

      Advanced Healthcare Materials

      Volume: 4 Issue: 2 Pages: 215-215

    • DOI

      10.1002/adhm.201400173

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Supramolecular flower micelle formation of polyrotaxane-containing triblock copolymers prepared from macro-chain transfer agent bearing molecular hooks2014

    • Author(s)
      A. Tamura, H. Tanaka, N. Yui.
    • Journal Title

      Polymer Chemistry

      Volume: 5 Issue: 15 Pages: 4511-4520

    • DOI

      10.1039/c4py00379a

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] 生体機能調節を目指したポリロタキサンの細胞内外からのアプローチ2016

    • Author(s)
      由井 伸彦
    • Organizer
      ポリマーフロンティア21 医療を支える機能性高分子材料の研究最前線
    • Place of Presentation
      東京工業大学蔵前会館(東京都目黒区)
    • Year and Date
      2016-04-21
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] ポリロタキサンを基盤とした新規仮着材の合成2016

    • Author(s)
      有坂慶紀, 松井七生子, 田村篤志, 高垣智博, 田上順次, 由井伸彦
    • Organizer
      第67回日本歯科理工学会学術講演会
    • Place of Presentation
      九州大学医学部百年講堂(福岡県福岡市)
    • Year and Date
      2016-04-16
    • Related Report
      2015 Annual Research Report
  • [Presentation] 光分解性ポリロタキサン架橋剤を用いた新規歯科用接着剤の開発2016

    • Author(s)
      伏見麻由, 徐知勲, 松井七生子, 有坂慶紀, 田村篤志, 高垣智博, 田上順次, 由井伸彦
    • Organizer
      第25回インテリジェント 材料・システムシンポジウム
    • Place of Presentation
      東京女子医科大学先端生命医科学研究所(東京都新宿区)
    • Year and Date
      2016-01-08
    • Related Report
      2015 Annual Research Report
  • [Presentation] Directing cellular functions via hydrated mobility of polyrotaxane surfaces2015

    • Author(s)
      Nobuhiko Yui, Ji-Hun Seo, Tetsuji Yamaoka, Sachiro Kakinoki,
    • Organizer
      International Chemical Congress of Pacific Basin Societies 2015 (Pacifichem 2015)
    • Place of Presentation
      Hawaii, USA
    • Year and Date
      2015-12-15
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 歯科用材料への応用を目指した光分解性ポリロタキサン架橋剤の合成と物性評価2015

    • Author(s)
      伏見麻由, 徐知勲, 由井伸彦
    • Organizer
      第64回高分子学会年次大会
    • Place of Presentation
      札幌コンベンションセンター(北海道札幌市)
    • Year and Date
      2015-05-25
    • Related Report
      2015 Annual Research Report
  • [Presentation] 動的ソフトマテリアル表面での細胞機能調節2014

    • Author(s)
      由井伸彦
    • Organizer
      生体医工学ワークショップ
    • Place of Presentation
      東京工業大学精密工学研究所 (神奈川県横浜市)
    • Year and Date
      2014-12-12 – 2014-12-13
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 超分子材料の動的特性を活かした細胞機能の調節2014

    • Author(s)
      由井伸彦
    • Organizer
      近畿化学協会機能性色素・エレクトロニクス部会東京地区合同講演会
    • Place of Presentation
      化学会館 7階ホール (東京都千代田区)
    • Year and Date
      2014-12-01
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 動的ポリロタキサン表面における間葉系幹細胞の分化特性評価2014

    • Author(s)
      徐知勲、柿木佐知朗、山岡哲二、由井伸彦
    • Organizer
      第36回日本バイオマテリアル学会大会
    • Place of Presentation
      タワーホール船堀 (東京都江戸川区)
    • Year and Date
      2014-11-17 – 2014-11-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] Supramolecular Biomaterials Exploit New Paradigm of Modulating Cellular Functions2014

    • Author(s)
      N. Yui
    • Organizer
      2014 Annual Meeting of the Korean Society for Biomaterials
    • Place of Presentation
      The Korean Federation of Science and Technology Societies (Korea)
    • Year and Date
      2014-11-06 – 2014-11-07
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] ポリロタキサンの動的骨格を活かした細胞機能の調節-新たな治療法を目指してー2014

    • Author(s)
      由井伸彦
    • Organizer
      第15回リング・チューブ超分子研究会シンポジウム
    • Place of Presentation
      東京工業大学大岡山キャンパス (東京都目黒区)
    • Year and Date
      2014-10-27 – 2014-10-28
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Directing cell fate by surface molecular mobility of supramolecular biomaterials2014

    • Author(s)
      N. Yui
    • Organizer
      JSPS A3 Foresight International Symposium on Nano-Biomaterials and Regenerative Medicine
    • Place of Presentation
      東京女子医科大学 (東京都新宿区)
    • Year and Date
      2014-10-08 – 2014-10-09
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Regulation of mesenchymal stem cell differentiation by changing the molecular structure of supramolecular surfaces2014

    • Author(s)
      J.-H. Seo, S. Kakinoki, T. Yamaoka, N. Yui
    • Organizer
      26th Annual Conference of the European Society for Biomaterials
    • Place of Presentation
      The Liverpool Echo Arena and Convention Centre (United Kingdom)
    • Year and Date
      2014-08-31 – 2014-09-03
    • Related Report
      2014 Annual Research Report
  • [Presentation] 分子運動性の異なるポリロタキサンの表面上における間葉系幹細胞の分化特性2014

    • Author(s)
      徐知勲、柿木佐知朗、山岡哲二、由井伸彦
    • Organizer
      第43回医用高分子シンポジウム
    • Place of Presentation
      産業技術総合研究所 臨海副都心センター (東京都江東区)
    • Year and Date
      2014-07-28 – 2014-07-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] Significance of supramolecular frames of CD- based polyrotaxanes as biomaterials: extracellular and intracellular approaches2014

    • Author(s)
      N. Yui
    • Organizer
      17th International Cyclodextrin Symposium
    • Place of Presentation
      Saarland University (Germany)
    • Year and Date
      2014-05-29 – 2014-05-31
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] ポリロタキサンの表面キャストによる間葉系幹細胞の分化制御2014

    • Author(s)
      徐知勲、柿木佐知朗、山岡哲二、由井伸彦
    • Organizer
      第63回高分子学会年次大会
    • Place of Presentation
      名古屋国際会議場 (愛知県名古屋市)
    • Year and Date
      2014-05-28 – 2014-05-30
    • Related Report
      2014 Annual Research Report
  • [Book] 表面・界面技術ハンドブック2016

    • Author(s)
      有坂慶紀, 由井伸彦
    • Total Pages
      796
    • Publisher
      エヌティーエス
    • Related Report
      2015 Annual Research Report
  • [Book] Biointerfaces: Where Material Meets Biology2014

    • Author(s)
      Ji-Hun Seo, Nobuhiko Yui
    • Total Pages
      13
    • Publisher
      Royal Society of Chemistry
    • Related Report
      2013 Annual Research Report

URL: 

Published: 2013-05-21   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi