| Project/Area Number |
25282143
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biomaterial science and engineering
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| Research Institution | University of Toyama |
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Principal Investigator |
Toshio Nikaido 富山大学, 事務局, 理事・副学長 (50180568)
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| Co-Investigator(Kenkyū-buntansha) |
YOSHIA TOSHIKO 富山大学, 大学院医学薬学研究部(医学), 准教授 (00171421)
OKABE MOTONORI 富山大学, 大学院医学薬学研究部(医学), 助教 (60283066)
KOIKE CHIKA 富山大学, 大学院医学薬学研究部(医学), 助教 (10523889)
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| Co-Investigator(Renkei-kenkyūsha) |
SAITHO SHIGERU 富山大学, 大学院医学薬学研究部(医学), 教授 (30175351)
Hayashi Astushi 富山大学, 大学院医学薬学研究部(医学), 教授 (20283773)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2015: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2014: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2013: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
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| Keywords | ヒト羊膜由来細胞 / 多能性幹細胞 / スフェア形成 / 免疫抑制作用 / NK細胞 / Monocyte / 抗炎症作用 / 乾燥羊膜 / 羊膜細胞 / 羊膜由来細胞 / 生体機能材料 |
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| Outline of Final Research Achievements |
Human amnion-derived cells(HAMD) are considered to be a promising alternative cell source for clinical use because of their proliferation and differentiation ability. The cells can easily be obtained from human amnion without medical intervention. HAMD express immunosuppressive factors CD59 and HLA-G, implying that they may have an immunosuppressive function. We investigated the effect of HAMD on NK cell and monocyte function. HAMD inhibited the cytotoxicity of NK cells to K562 cells, depended on the NK/ HAMD ratio. The inhibition of NK cytotoxicity was recovered by continuous culturing without HAMD. This inhibition was related to the decreasing of the activated NK receptors and the production of IFN-γ in NK cells, to the increasing of the expression of IL-10 and PGE2 in HAMD. Antibody to IL-10 or PGE2 inhibitor recovered the NK cytotoxicity. HAMD also suppressed the activity of TNF-α and IL-6 in monocytes. These data suggested that amniotic cells have immunosuppressive activity.
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