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Efficient and durable transgene expression systems

Research Project

Project/Area Number 25282144
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Biomedical engineering/Biomaterial science and engineering
Research InstitutionHiroshima University (2014-2015)
Ehime University (2013)

Principal Investigator

Kamiya Hiroyuki  広島大学, 医歯薬保健学研究院(薬), 教授 (10204629)

Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥15,600,000 (Direct Cost: ¥12,000,000、Indirect Cost: ¥3,600,000)
Fiscal Year 2015: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
Keywords遺伝子治療 / プラスミドDNA
Outline of Final Research Achievements

Histone acetylation is associated with activation of genes on chromosomes. Transgene expression from plasmid DNA might be increased when histone bound to plasmid DNA is acetylated. We employed the positive feedback system using a fusion protein of the sequence-specific DNA binding domain of yeast GAL4 and the histone acetyltransferase (HAT) domain of mouse CREB-binding protein (GAL4-HAT), in which GAL4-HAT promotes its own expression as well as that of a reporter gene product (luciferase), to examine the hypothesis. The activator plasmid DNA carrying the gene for GAL4-HAT was introduced into mouse Hepa1-6 cells together with the reporter plasmid DNA by lipofection. Significantly increased luciferase expression was observed by the co-introduction of the activator plasmid DNA. Moreover, acetylation of histone bound to the reporter plasmid DNA was enriched by the activator plasmid DNA. These results indicated that the GAL4-HAT is useful for enhanced transgene expression.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • Research Products

    (16 results)

All 2016 2015 2014 2013 Other

All Journal Article (4 results) (of which Peer Reviewed: 4 results,  Open Access: 2 results,  Acknowledgement Compliant: 2 results) Presentation (12 results) (of which Invited: 2 results)

  • [Journal Article] Insertion and deletion mismatches distant from the target position improve gene correction with a tailed duplex.2016

    • Author(s)
      H. Kamiya*, N. Nishigaki, A. Ikeda, S. Yukawa, Y. Morita, Y. Nakatsu, T. Tsuzuki and H. Harashima
    • Journal Title

      Nucleosides Nucleotides Nucleic Acids

      Volume: 35 Issue: 7 Pages: 379-388

    • DOI

      10.1080/15257770.2016.1163384

    • NAID

      120006313423

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Transcription of 4’-thioDNA templates to natural RNA in vitro and in mammalian cells.2015

    • Author(s)
      Maruyama, H.; Furukawa, K.; Kamiya, H.; Minakawa, N.; Matsuda, A.
    • Journal Title

      Chem. Commun.

      Volume: 51 Issue: 37 Pages: 7887-7890

    • DOI

      10.1039/c4cc08862j

    • NAID

      120005714777

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Anatomy of plasmid DNAs with anti-silencing elements2014

    • Author(s)
      G. N. Kanda, S. Miyamoto, M. Kobayashi, I. Matsuoka, H. Harashima and H. Kamiya
    • Journal Title

      Int. J. Pharm.

      Volume: 464 Issue: 1-2 Pages: 27-33

    • DOI

      10.1016/j.ijpharm.2014.01.025

    • Related Report
      2014 Annual Research Report 2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Correlation between transgene expression and plasmid DNA loss in mouse liver2013

    • Author(s)
      R. Togashi, H. Harashima and H. Kamiya
    • Journal Title

      J. Gene Med.

      Volume: 15 Issue: 6-7 Pages: 242-248

    • DOI

      10.1002/jgm.2716

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Presentation] 特異的ヒストンアセチル化による導入プラスミドDNAの発現上昇2015

    • Author(s)
      西原実香,鈴木哲矢,紙谷浩之
    • Organizer
      第38回日本分子生物学会年会・第88回日本生化学会大会合同大会
    • Place of Presentation
      神戸
    • Year and Date
      2015-12-03
    • Related Report
      2015 Annual Research Report
  • [Presentation] 非標的部位に導入した塩基-塩基ミスマッチがtailed duplexによる配列変換へ与える影響2015

    • Author(s)
      西垣奈津希,鈴木哲矢,中津可道,續輝久,紙谷浩之
    • Organizer
      第38回日本分子生物学会年会・第88回日本生化学会大会合同大会
    • Place of Presentation
      神戸
    • Year and Date
      2015-12-03
    • Related Report
      2015 Annual Research Report
  • [Presentation] 標的配列近傍での切断は5’-tailed duplexによる配列変換効率を向上させる2015

    • Author(s)
      今田貴士,西垣奈津希,鈴木哲矢,紙谷浩之
    • Organizer
      第38回日本分子生物学会年会・第88回日本生化学会大会合同大会
    • Place of Presentation
      神戸
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
  • [Presentation] 核酸を用いる発癌・制癌研究:DNA損傷による変異機構の解明と遺伝子治療用核酸の設計2015

    • Author(s)
      紙谷浩之
    • Organizer
      日本分析化学会中国四国支部 広島地区分析技術研究会
    • Place of Presentation
      東広島
    • Year and Date
      2015-03-18
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 非標的部位におけるミスマッチの配列変換(遺伝子修復)への影響2014

    • Author(s)
      西垣奈津希,池田彰弘,湯川誠也,守田由子,中津可道,續輝久,原島秀吉,紙谷浩之
    • Organizer
      第37回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2014-11-26
    • Related Report
      2014 Annual Research Report
  • [Presentation] 特異的ヒストン修飾による導入プラスミドDNAの発現上昇2014

    • Author(s)
      西原実香,神田元紀,山門振一郎,原島秀吉,紙谷浩之
    • Organizer
      第37回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2014-11-26
    • Related Report
      2014 Annual Research Report
  • [Presentation] DNA損傷が誘発する発癌機構の解明と遺伝子治療用核酸の設計2014

    • Author(s)
      紙谷浩之
    • Organizer
      日本分析化学会第63年会
    • Place of Presentation
      東広島
    • Year and Date
      2014-09-18
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] プラスミドDNA特異的ヒストン修飾による導入遺伝子発現上昇2014

    • Author(s)
      西原実香,神田元紀,山門振一郎,原島秀吉,紙谷浩之
    • Organizer
      第30回日本DDS学会学術集会
    • Place of Presentation
      東京
    • Year and Date
      2014-07-30
    • Related Report
      2014 Annual Research Report
  • [Presentation] 導入プラスミドDNAの特異的ヒストン修飾による発現上昇2014

    • Author(s)
      西原実香,神田元紀,山門振一郎,原島秀吉,紙谷浩之
    • Organizer
      第55回日本生化学会中国・四国支部例会
    • Place of Presentation
      松山
    • Year and Date
      2014-06-07
    • Related Report
      2014 Annual Research Report
  • [Presentation] ファージDNAを用いたtailed duplexの作製と標的遺伝子の配列変換2014

    • Author(s)
      西垣奈津希,池田彰弘,湯川誠也,守田由子,中津可道,續輝久,原島秀吉,紙谷浩之
    • Organizer
      第55回日本生化学会中国・四国支部例会
    • Place of Presentation
      松山
    • Year and Date
      2014-06-07
    • Related Report
      2014 Annual Research Report
  • [Presentation] The effect of plasmid DNA sequence on gene expression and polycation complex formation

    • Author(s)
      R. Togashi, H. Akita, H. Kamiya, H. Harashima
    • Organizer
      40th Annual Meeting & Exposition of the Controlled Release Society
    • Place of Presentation
      Honolulu, Hawaii, USA
    • Related Report
      2013 Annual Research Report
  • [Presentation] In vivoにおける外来遺伝子発現の持続を達成するDNA配列の同定

    • Author(s)
      神田元紀,小林三和子,松岡一郎,原島秀吉,紙谷浩之
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Related Report
      2013 Annual Research Report

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Published: 2013-05-21   Modified: 2019-07-29  

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