| Project/Area Number |
25282145
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biomaterial science and engineering
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| Research Institution | Tokyo Women's Medical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
NAGASE Kenichi 東京女子医科大学, 医学部, 講師 (10439838)
AKIMOTO Jun 東京女子医科大学, 医学部, 助教 (80649682)
IWASE YUKO 東京女子医科大学, 医学部, 博士研究員 (80406371)
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| Research Collaborator |
KANNO Tomonori
TOYOSHIMA Yuki
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2015: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2014: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2013: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
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| Keywords | がん / 細胞シート / 組織工学 / がんモデル / 薬剤応答性 / 薬剤反応性 / 癌 / バイオイメージング / バイオリアクター |
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| Outline of Final Research Achievements |
Novel cancer tissue engineering has been developed using sheet-like cancer cell structures, cancer cell sheets, to overcome current artificial cancer fabrication methods for anticancer drug screening. We transplanted cancer cell sheets to small animals and clarified effective tumor formation. In addition, orthotopic liver cancer models were successfully achieved by transplantation of hepatocarcinoma sheet to host liver tissue. Moreover, an in vitro tumor model was constructed by sandwiching a cancer cell sheet between two collagen layers as a biomimicking tumor tissue. In the biomimicking tumor tissue model, hypoxic areas were observed, and the secretion of vascular endothelial growth factor increased time-dependently. Cell growth inside the model was slower than that of conventional culture system. Cytotoxicity of doxorubicin with the model decreased compared with the conventional system, suggesting that the surrounding collagen layer mimicked in vivo tumor structure.
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