Development of a high performance system to assess the mitochondrial respiratory activity and embryo quality based on scanning electrochemical microscopy
| Project/Area Number |
25282150
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Medical systems
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| Research Institution | Yamagata University |
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Principal Investigator |
Abe Hiroyuki 山形大学, 大学院理工学研究科, 教授 (10375199)
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| Co-Investigator(Kenkyū-buntansha) |
黒谷 玲子 山形大学, 理工学研究科, 准教授 (00453043)
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| Research Collaborator |
TAKAKURA Kei
SAKAHARA Satoshi
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥15,860,000 (Direct Cost: ¥12,200,000、Indirect Cost: ¥3,660,000)
Fiscal Year 2015: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2014: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2013: ¥9,490,000 (Direct Cost: ¥7,300,000、Indirect Cost: ¥2,190,000)
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| Keywords | 細胞呼吸 / 電気化学計測 / 細胞機能診断 / ミトコンドリア / 生殖医療 |
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| Outline of Final Research Achievements |
Respiration is useful parameter for evaluating embryo quality as it provides important information about metabolic activity. In this study, we have developed a scanning electrochemical microscopy (SECM) technique that is a non-invasive and sensitive method for measuring oxygen consumption by individual oocytes and embryos. With this system, oxygen consumption of single, identical oocytes and embryos at different developmental stages has been monitored. This study showed that the mitochondrial respiratory activity in embryos increased in morula and peaked in hatched blastocyst and the development of mitochondrial function depends on the gene expression of COX subunit encoded by genomic DNA. This study shows that the SECM technique is able to non-invasively assess the mitochondrial respiration function of individual bovine embryos and the embryo quality. The SECM system may contribute to improvements in reproductive technologies.
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Report
(4 results)
Research Products
(89 results)
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[Journal Article] Hydroxypropyl cellulose as an option for supplementation of cryoprotectant solutions for embryo vitrification in human assisted reproductive technologies2015
Author(s)
Mori C., Yabuuchi A., Ezoe K., Murata N., Takayama Y., Okimura T., Uchiyama K., Takakura K., Abe H., Wada K., Okuno T., TKobayashi T., Kato K.
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Journal Title
Reproductive BioMedicine Online
Volume: 30
Issue: 6
Pages: 613-621
DOI
Related Report
Peer Reviewed
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[Presentation] Respiratory activity of the human embryos monitored by the automated device with the chip electrode2015
Author(s)
Shiga N., Utsunomiya H., Kurosawa H., Takahashi A., Ishibashi M., Watanabe Z., Abe H., Terada Y., Takahashi T., Fukui A., Suganuma R., Yaegashi N.
Organizer
IFFS/JSRM International Meeting 2015
Place of Presentation
Pacifico Yokohama, Yokohama, Japan
Year and Date
2015-04-26
Related Report
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[Presentation] Investigation of oxygen consumption rate of bovine embryos measured by chip electrode2015
Author(s)
Kurosawa H., Utsunomiya H., Takahashi A., Ishibashi M., Nishimoto M., Shiga N., Watanabe Z., Abe H., Terada Y., Suganuma R., Yaegashi N.
Organizer
IFFS/JSRM International Meeting 2015
Place of Presentation
Pacifico Yokohama, Yokohama, Japan
Year and Date
2015-04-26
Related Report
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[Presentation] Novel automated device with the chip electrode for monitoring respiratory activity of embryos2014
Author(s)
Kurosawa H., Utsunomiya H., Shiga N., Takahashi A., Ishibashi M., Watanabe Z., Abe H., Terada Y., Takahashi T., Fukui A., Suganuma R., Yaegashi N.
Organizer
2014 Annual Meeting of American Society for Reproductive Medicine
Place of Presentation
Honolulu, Hawaii, USA
Year and Date
2014-09-28
Related Report
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