| Budget Amount *help |
¥15,860,000 (Direct Cost: ¥12,200,000、Indirect Cost: ¥3,660,000)
Fiscal Year 2015: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2014: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2013: ¥9,490,000 (Direct Cost: ¥7,300,000、Indirect Cost: ¥2,190,000)
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| Outline of Final Research Achievements |
Respiration is useful parameter for evaluating embryo quality as it provides important information about metabolic activity. In this study, we have developed a scanning electrochemical microscopy (SECM) technique that is a non-invasive and sensitive method for measuring oxygen consumption by individual oocytes and embryos. With this system, oxygen consumption of single, identical oocytes and embryos at different developmental stages has been monitored. This study showed that the mitochondrial respiratory activity in embryos increased in morula and peaked in hatched blastocyst and the development of mitochondrial function depends on the gene expression of COX subunit encoded by genomic DNA. This study shows that the SECM technique is able to non-invasively assess the mitochondrial respiration function of individual bovine embryos and the embryo quality. The SECM system may contribute to improvements in reproductive technologies.
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