| Project/Area Number |
25282164
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Rehabilitation science/Welfare engineering
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| Research Institution | Tokoha University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
AGATA Nobuhide 常葉大学, 保健医療学部, 講師 (00549313)
HOKAMURA Kazuya 浜松医科大学, 医学部, 助教 (90436965)
KUMADA Tatsuro 常葉大学, 保健医療学部, 講師 (00402339)
UMEMURA Kazuo 浜松医科大学, 医学部, 教授 (40232912)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000)
Fiscal Year 2015: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
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| Keywords | 実験的脳梗塞 / 神経新生 / 運動負荷 / 神経系細胞分化 / リハビリテーション / 脳梗塞 / 発生・分化 / 脳・神経 / 神経発生 |
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| Outline of Final Research Achievements |
Although exercises enhance functional recovery from the strokes, it is not known about the cellular mechanisms. We made brain infarction by surgical occlusion of the left median cerebral artery. After the strokes, the rats were injected with 5-bromo-2’-deoxyuridine (BrdU) and then given exercise by treadmill and sacrificed 1 week (W), 4W and 8W. The brains were compared between exercised and non-exercised groups by immune-staining using antibodies against BrdU and Sox2 (immature neural marker). Lots of immature cells were moved from the subventricular zone (SVZ) to the peri-infarcted regions. We could not detect significant difference of the moves between the groups. However, BrdU-positive cells were significantly increased in the separated cortex from the infarcted lesions in the exercised group. The numbers of BrdU-positive cells were remarkably decreased by the 8W, suggesting that most of the BrdU-positive cells does not differentiate to matured neuron.
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