The role of microRNAs in mechanical stress-induced muscle adaptation

• Project/Area Number: 25282198
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Sports science
• Research Institution: The University of Tokyo
• Principal Investigator: AKIMOTO Takayuki 東京大学, 医学(系)研究科(研究院), 講師 (00323460)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000); Fiscal Year 2015: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2014: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000); Fiscal Year 2013: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
• Keywords: 筋線維タイプ / 転写後制御 / ノンコーディングRNA / 可塑性 / メカニカルストレス / Dicer / microRNA / 遅筋 / 速筋 / ミトコンドリア / マイクロRNA / 骨格筋 / 適応

Outline of Final Research Achievements

We investigated the role of microRNA on mechanical stress-induced muscle adaptation by using Dicer1 knock-out mice (Dicer1 KO). As a result, the muscle-specific Dicer1KO mice died by 6 weeks due to dysfunction of muscles, suggesting that microRNAs in muscle play important roles in homeostasis of muscles.

Research Products

• [Journal Article] MicroRNA-23a has minimal effect on endurance exercise-induced adaptation of mouse skeletal muscle. (2015)
  • Author(s): S. Wada, Y. Kato, S. Sawada, K. Aizawa, J. H. Park, A. P. Russell, T. Ushida, T. Akimoto
  • Journal Title: Pflugers Archiv, Volume: 467 Pages: 389-398
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] The microRNA-23a has limited roles in bone formation and homeostasis in vivo. (2015)
  • Author(s): J. H. Park, S. Wada*, T. Ushida, T. Akimoto
  • Journal Title: Physiol Res Volume: 64 Pages: 711-716
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Mesenchymal-stem-cell-derived exosomes containing microRNAs accelerate skeletal muscle regeneration. (2015)
  • Author(s): Y. Nakamura*, S. Miyaki*, H. Ishitobi, S. Matsuyama, T. Nakasa, N. Kamei, T. Akimoto, Y. Higashi, M. Ochi.
  • Journal Title: FEBS Letters Volume: 589 Pages: 1257-1265
  • Peer Reviewed
• [Journal Article] MicroRNA-23a has minimal effect on endurance exercise-induced adaptation of mouse skeletal muscle. (2015)
  • Author(s): S. Wada, Y. Kato, S. Sawada, K. Aizawa, J. H. Park, A. P. Russell, T. Ushida, T. Akimoto.
  • Journal Title: Pflügers Archiv Volume: 467 Pages: 389-398
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] The microRNA-23a has limited roles in bone formation and homeostasis in vivo. (2015)
  • Author(s): J. H. Park, S. Wada, T. Ushida, T. Akimoto.
  • Journal Title: Physiol Res Volume: x
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Effect of aging and sex on circulating microRNAs in humans. (2014)
  • Author(s): S. Sawada, T. Akimoto, M. Takahashi, R. Sakurai, S. Shinkai, T. Ushida, Y. Fujiwara, K. Suzuki.
  • Journal Title: Adv Aging Res Volume: 3 Pages: 152-159
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Regulation of miRNAs in human skeletal muscle following acute endurance exercise and short term endurance training. (2013)
  • Author(s): A. P. Russell, S. Lamon, H. Boon, S. Wada, I. Guller, E. L. Brown, A. V. Chibalin, J. Zierath, R. J. Snow, N. K. Stepto, G. D. Wadley, T. Akimoto.
  • Journal Title: J Physiol Volume: 591 Pages: 4637-4653
  • Peer Reviewed
• [Journal Article] Profiling of circulating microRNAs after a bout of acute resistance exercise in humans. (2013)
  • Author(s): S. Sawada, M. Kon, S. Wada, T. Ushida, K. Suzuki, T. Akimoto.
  • Journal Title: PLoS One Volume: 8
  • Peer Reviewed
• [Presentation] 細胞間コミュニケーションツールとしてのマイクロRNA. (2015)
  • Author(s): 秋本崇之
  • Organizer: 第66回日本体育学会
  • Place of Presentation: 東京
  • Year and Date: 2015-08-10
  • Invited
• [Presentation] MicroRNA-23aは持久性運動による筋適応に関与しない. (2014)
  • Author(s): 秋本崇之, 和田正吾, 加藤義雄, 沢田秀司, 相澤勝治, 朴鐘薫, 牛田多加志.
  • Organizer: 第69回日本体力医学会学術総会
  • Place of Presentation: 長崎
  • Year and Date: 2014-09-20 – 2014-09-22
• [Presentation] MicroRNAs in muscle plasticity. (2014)
  • Author(s): T. Akimoto
  • Organizer: Brain Korea 21 plus conference in Dankook University.
  • Place of Presentation: Chonan
  • Year and Date: 2014-06-11 – 2014-06-13
  • Invited
• [Presentation] 骨格筋可塑性に関わるマイクロRNA. (2013)
  • Author(s): 秋本崇之
  • Organizer: 第5回分子骨格筋代謝研究会
  • Place of Presentation: 京都
  • Invited