Creation of a new light-dependent gene expression system
| Project/Area Number |
25282229
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biomolecular chemistry
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
Masuda Shinji 東京工業大学, バイオ研究基盤支援総合センター, 准教授 (30373369)
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| Co-Investigator(Kenkyū-buntansha) |
Tanaka Mikiko 東京工業大学, 生命理工学研究科, 准教授 (40376950)
Asano Tsunaki 首都大学, 東京理工学研究科, 助教 (40347266)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2015: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2014: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2013: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
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| Keywords | 遺伝子発現制御 / 光受容体 / オプトジェネティクス / BLUF / 遺伝子発現 / 光遺伝学 / 転写制御 |
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| Outline of Final Research Achievements |
In this study, we aimed to construct a novel light-dependent gene expression system. We utilized the light-dependent protein-protein interaction between the blue-light photoreceptor PixD and its interacting protein PixE. We found that PixD interacts with the PixE N-terminal region. The N-terminal region of PixE was fused with the transcription factor AGAMOUS of the model plant Arabidopsis. Previous studies showed that AGAMOUS is necessary for normal flower formation. Thus, blue-light-dependent modulation of AGAMOUS activity could be monitored easily by phenotypic analysis. Biochemical analysis showed that PixD interacts with the chimeric transcription factor, indicating that PixD may control the chimeric transcription factor activity also in vivo. To check this hypothesis, we produce transgenic Arabidopsis expressing constitutively PixD and/or the chimeric transcription factor.
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Report
(4 results)
Research Products
(24 results)
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[Journal Article] Efficient and cost effective production of active-form human PKB using silkworm larvae.2014
Author(s)
R. Maesaki, R. Satoh, M. Taoka, T. Kanaba, T. Asano, C. Fujita, T. Fujiwara, Y. Ito, T. isobe, T. Hakoshima, K. Maenaka, and M. Mishima
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Journal Title
Scientific Reports
Volume: 4
Issue: 1
Pages: 6016-6016
DOI
Related Report
Peer Reviewed / Open Access
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