| Budget Amount *help |
¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2015: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2014: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2013: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
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| Outline of Final Research Achievements |
In this study, we aimed to construct a novel light-dependent gene expression system. We utilized the light-dependent protein-protein interaction between the blue-light photoreceptor PixD and its interacting protein PixE. We found that PixD interacts with the PixE N-terminal region. The N-terminal region of PixE was fused with the transcription factor AGAMOUS of the model plant Arabidopsis. Previous studies showed that AGAMOUS is necessary for normal flower formation. Thus, blue-light-dependent modulation of AGAMOUS activity could be monitored easily by phenotypic analysis. Biochemical analysis showed that PixD interacts with the chimeric transcription factor, indicating that PixD may control the chimeric transcription factor activity also in vivo. To check this hypothesis, we produce transgenic Arabidopsis expressing constitutively PixD and/or the chimeric transcription factor.
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