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Optical control of cellular functions by PCDR technology

Research Project

Project/Area Number 25282232
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Biomolecular chemistry
Research InstitutionOkayama University

Principal Investigator

Ohtsuki Takashi  岡山大学, 自然科学研究科, 教授 (80321735)

Co-Investigator(Kenkyū-buntansha) WATANABE KAZUNORI  岡山大学, 大学院自然科学研究科, 助教 (70602027)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2015: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2014: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2013: ¥8,970,000 (Direct Cost: ¥6,900,000、Indirect Cost: ¥2,070,000)
Keywords核酸 / RNA / 光誘導 / 翻訳 / 細胞分化
Outline of Final Research Achievements

In this study, methods to control cellular functions by visible and infrared light were developed based on “Photoinduced Cytosolic Dispersion of RNA (PCDR) technology” for introducing RNAs into cytoplasm only within irradiated cells. Optimization of PCDR using infrared light, temporal control of introduction of two kinds of RNAs, the application to speroid (three-dimensional multicell model), and optical control of the cell functions have been archived.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • Research Products

    (23 results)

All 2016 2015 2014 2013

All Journal Article (8 results) (of which Peer Reviewed: 7 results,  Open Access: 2 results) Presentation (13 results) (of which Invited: 5 results) Book (2 results)

  • [Journal Article] Photocontrolled Intracellular RNA Delivery Using Nanoparticles or Carrier-Photosensitizer Conjugates.2016

    • Author(s)
      Watanabe K., Ohtsuki T.
    • Journal Title

      Prog Mol Biol Transl Sci.

      Volume: 139 Pages: 101-119

    • DOI

      10.1016/bs.pmbts.2015.10.013

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The molecular mechanism of photochemical internalization of cell penetrating peptide-cargo-photosensitizer conjugates2015

    • Author(s)
      Takashi Ohtsuki, Shunya Miki, Shouhei Kobayashi, Tokuko Haraguchi, Eiji Nakata, Kazutaka Hirakawa, Kensuke Sumita, Kazunori Watanabe, Shigetoshi Okazaki
    • Journal Title

      Scientific Reports

      Volume: 5 Issue: 1 Pages: 18577-18577

    • DOI

      10.1038/srep18577

    • NAID

      120005844166

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 光化学的に細胞質内に侵入するペプチド分子の設計2015

    • Author(s)
      大槻高史
    • Journal Title

      月刊 化学工業

      Volume: 66 Pages: 34-39

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Novel leucine zipper motif-based hybrid peptide delivers functional peptide cargo inside cells.2015

    • Author(s)
      Hakata, Y., S. Tsuchiya, H. Michiue, T. Ohtsuki, H. Matsui, M. Miyazawa, and M. Kitamatsu
    • Journal Title

      Chem. Comm.

      Volume: 51 Issue: 2 Pages: 413-416

    • DOI

      10.1039/c4cc07459a

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Photo-dependent protein biosynthesis using a caged aminoacyl-tRNA2014

    • Author(s)
      Akahoshi, A., Doi, Y., Sisido, M., Watanabe, K., Ohtsuki, T.
    • Journal Title

      Bioorganic & Medicinal Chemistry Letters

      Volume: 24 Pages: 5369-5372

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] mTOR regulates the nucleoplasmic diffusion of Xrn2 under conditions of heat stress.2014

    • Author(s)
      Watanabe, K., Ijiri K., Ohtsuki, T.
    • Journal Title

      FEBS Letters

      Volume: 588 Issue: 18 Pages: 3454-3460

    • DOI

      10.1016/j.febslet.2014.08.003

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Near-infrared light-directed RNAi using a photosensitive carrier molecule.2013

    • Author(s)
      Matsushita-Ishiodori, Y., Morinaga, M., Watanabe, K., Ohtsuki, T.
    • Journal Title

      Bioconjugate Chemistry

      Volume: 24 Issue: 10 Pages: 1669-1673

    • DOI

      10.1021/bc4001195

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Synthesis and Properties of Peptide Dendrimers Containing Fluorescent and Branched Amino Acids. Biopolymers2013

    • Author(s)
      Kitamatsu, M., Kitabatake, M., Noutoshi, Y.,Ohtsuki, T.
    • Journal Title

      Peptide Science

      Volume: 100 Issue: 1 Pages: 64-70

    • DOI

      10.1002/bip.22175

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Presentation] Photosensitizing peptides and proteins for photochemical internalization2015

    • Author(s)
      Takashi Ohtsuki, Shunya Miki, Hayato Fujiwara, Mizuki Kitamatsu, Kazutaka Hirakawa, Shigetoshi Okazaki, Kazunori Watanabe
    • Organizer
      Pacifichem 2015
    • Place of Presentation
      Honolulu,Hawaii
    • Year and Date
      2015-12-16
    • Related Report
      2015 Annual Research Report
  • [Presentation] 可視光および近赤外光による細胞内RNA導入の局所的誘導2015

    • Author(s)
      大槻高史、白神かおり、渡邉和則
    • Organizer
      BMB2015
    • Place of Presentation
      神戸
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 新規神経分化関連microRNAの探索2015

    • Author(s)
      山路隆平、渡邊和則、大槻高史
    • Organizer
      BMB2015
    • Place of Presentation
      神戸
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
  • [Presentation] 光を用いた細胞内への物質導入2015

    • Author(s)
      大槻 高史
    • Organizer
      新学術領域研究「ナノメディシン分子科学」+「超高速バイオアセンブラ」合同若手の会
    • Place of Presentation
      大阪
    • Year and Date
      2015-10-31
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] アポトーシスの光誘導法の開発2015

    • Author(s)
      藤原 隼人・畑地 祐里・北松 瑞生・渡邉 和則・大槻 高史
    • Organizer
      第9回バイオ関連化学シンポジウム
    • Place of Presentation
      熊本
    • Year and Date
      2015-09-10
    • Related Report
      2015 Annual Research Report
  • [Presentation] Design of photosensitizing peptide molecules for photochemical internalization2015

    • Author(s)
      Takashi Ohtsuki, Hayato Fujiwara, Yuri Hatachi, Mizuki Kitamatsu, Kazunori Watanabe
    • Organizer
      The 6th Taiwan-Japan Symposium on Nanomedicine
    • Place of Presentation
      Taipei
    • Year and Date
      2015-01-08 – 2015-01-09
    • Related Report
      2014 Annual Research Report
  • [Presentation] PCI法におけるエンドソーム脱出機構の解明2014

    • Author(s)
      三木 駿也, 澄田 憲祐, 渡邉 和則, 平川 和貴, 岡崎 茂俊, 大槻 高史
    • Organizer
      第 37 回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2014-11-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] CLIP-RNAi法で高効率にRNAiを起こす多環状RNAの開発2014

    • Author(s)
      村上 真一, 渡邉 和則, 大槻 高史
    • Organizer
      第 37 回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2014-11-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] 光化学的に細胞質内に侵入するペプチド分子の設計2014

    • Author(s)
      大槻 高史・藤原 隼人・畑地 祐里・北松 瑞生・渡邉 和則
    • Organizer
      光化学討論会
    • Place of Presentation
      札幌
    • Year and Date
      2014-10-11
    • Related Report
      2014 Annual Research Report
  • [Presentation] 光誘導RNAi法 (CLIP-RNAi) の3次元細胞集合体への応用2014

    • Author(s)
      岩崎慎也、渡邉和則、中澤浩二、大槻高史
    • Organizer
      第2回バイオ関連化学シンポジウム若手フォーラム
    • Place of Presentation
      岡山
    • Year and Date
      2014-09-10
    • Related Report
      2014 Annual Research Report
  • [Presentation] 光に応答する生体機能分子2014

    • Author(s)
      大槻高史
    • Organizer
      第2回 日本バイオマテリアル学会中四国シンポジウム
    • Place of Presentation
      岡山
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Light-directed RNAi using a photosensitive carrier molecule2013

    • Author(s)
      Takashi Ohtsuki
    • Organizer
      International Symposium on Nanomedicine Molecular Science
    • Place of Presentation
      Tokyo
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 光で細胞機能を操る2013

    • Author(s)
      大槻高史
    • Organizer
      テラヘルツと生命科学融合による革新的イノベーションワークショップ
    • Place of Presentation
      岡山
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Book] Fundamental Biomedical Technology (Chapter 19を担当)2014

    • Author(s)
      Watanabe K. and Ohtsuki T.
    • Total Pages
      479
    • Publisher
      Springer
    • Related Report
      2014 Annual Research Report
  • [Book] Intracellular Delivery (Fundamental Biomedical Technology, Vol 7)2014

    • Author(s)
      Watanabe K. and Ohtsuki T.(部分執筆, chapter 19)
    • Total Pages
      490
    • Publisher
      Springer
    • Related Report
      2013 Annual Research Report

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Published: 2013-05-21   Modified: 2019-07-29  

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