Elucidation of Mechanisms on Membrane Protein Integration by a Glycolipid Acting Like an Enzyme
| Project/Area Number |
25282235
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biomolecular chemistry
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| Research Institution | Suntory Foundation for Life Sciences |
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Principal Investigator |
SHIMAMOTO Keiko 公益財団法人サントリー生命科学財団, その他部局等, その他 (70235638)
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| Co-Investigator(Renkei-kenkyūsha) |
MIURA Kaoru (Nomura Kaoru) (公益財団法人)サントリー生命科学財団, 生物有機科学研究所, 研究員 (90353515)
NISHIYAMA Ken-ichi 岩手大学, 農学部, 教授 (80291334)
MURATA Michio 大阪大学, 大学院理学研究科, 教授 (40183652)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2015: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2014: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
Fiscal Year 2013: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
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| Keywords | 糖脂質 / 膜挿入 / 膜タンパク質 / 糖鎖合成 / 生体膜 / 生合成基質 / シャペロン / 糖鎖 |
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| Outline of Final Research Achievements |
We previously reported an integration factor in the inner membrane of E. coli, named MPIase (membrane protein integrase) is a glycolipid composed of diacylglycerol and a glycan chain of three acetylated aminosugars linked through pyrophosphate. MPIase is essential for membrane protein integration and acts like an enzyme. In this study, we showed that O-acetyl groups in the glycan and a phosphate group are required for its activity. NMR experiments indicated the interaction between the acetyl groups of MPIase and the substrate peptide. Based on these results, we propose a plausible mode of action, in which a glycan chain of MPIase captures a substrate to prevent aggregation. Moreover, we synthesized a trisaccharide unit of MPIase to reveal a minimum active structure.
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Report
(4 results)
Research Products
(23 results)
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[Journal Article] Kinetic resolution and stereoselective synthesis of 3-substituted aspartic acids by using engineered methylaspartate ammonia lyases2013
Author(s)
Raj, H., Szymanski, W., de Villiers, J., Puthan Veetil, V., Quax, W.J., Shimamoto, K., Janssen D. B., Feringa, B. L., Poelarends, G. J.
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Journal Title
Chemistry
Volume: 19
Issue: 34
Pages: 11148-11152
DOI
Related Report
Peer Reviewed
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