| Budget Amount *help |
¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000)
Fiscal Year 2015: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2014: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2013: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
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| Outline of Final Research Achievements |
In central nervous systems, excitatory neurotransmission is essential for our memory and learning. Glutamate, a typical excitatory neurotransmitter in animals, is recognized by glutamate receptors in synapses. Glutamate receptors are categorized into ionotropic- or metabotropic-types. These are composed of 25 subtypes, each of which has essential roles in neurotransmission and synaptic plasticity, a putative molecular mechanism of our memory. For subtype-selective activation of glutamate receptors, we here developed a novel method for selective activation of the receptor subtypes using genetic manipulation and coordination chemistry,. In this method, we introduced two His mutations in the vicinity of the ligand binding pockets in glutamate receptors. These His mutations acted as an allosteric site upon complexation with a Pd complex in a bidentate coordination fashion to stabilize the active conformation of the ligand binding pockets.
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