Development of RNA medicine based on nano-structured design and templated reaction

• Project/Area Number: 25282240
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Chemical biology
• Research Institution: Nagoya University (2015); Hokkaido University
• Principal Investigator: ABE HIROSHI 名古屋大学, 理学(系)研究科(研究院), 教授 (80415067)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000); Fiscal Year 2015: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000); Fiscal Year 2014: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000); Fiscal Year 2013: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
• Keywords: RNA / 化学反応 / 免疫応答 / RNA干渉 / 細胞

Outline of Final Research Achievements

We developed a new strategy for the buildup reaction of active siRNA species from short RNA fragments in living cells using a chemical ligation reaction. This strategy could decrease undesired immune responses and　provide more latitude for　RNAi technology in the design and concentration　of introduced RNA compared to traditional siRNA methods.

Research Products

• [Journal Article] Rolling Circle Translation of Circular RNA in Living Human Cells. (2015)
  • Author(s): N. Abe, K. Matsumoto, M. Nishihara, Y. Nakano, A. Shibata, H. Maruyama, S. Shuto, A. Matsuda, M. Yoshida, Y. Ito, H. Abe
  • Journal Title: Scientific Rep Volume: 15 Issue: 1 Pages: 16435-16435
  • DOI: 10.1038/srep16435
  • Peer Reviewed / Open Access
• [Journal Article] Phosphorylated 5-ethynyl-2'-deoxyuridine for advanced DNA labeling (2015)
  • Author(s): 1.Seo, Siyoong; Onizuka, Kazumitsu; Nishioka, Chieko; Takahashi, Eiki; Tsuneda, Satoshi; Abe, Hiroshi; Ito, Yoshihiro
  • Journal Title: Organic & Biomolecular Chemistry Volume: 13 Issue: 15 Pages: 4589-4595
  • DOI: 10.1039/c5ob00199d
  • Peer Reviewed
• [Journal Article] Nickel-Catalyzed Suzuki-Miyaura Coupling of a Tertiary Iodocyclopropane with Wide Boronic Acid Substrate Scope: Coupling Reaction Outcome Depends on Radical Species Stability (2015)
  • Author(s): Keisuke Yotsuji, Naoyuki Hoshiya, Takaaki Kobayashi, Hayato Fukuda, Hiroshi Abe, Mitsuhiro Arisawa and Satoshi Shuto
  • Journal Title: Adv. Synth. Catal. Volume: 357 Issue: 5 Pages: 1022-1028
  • DOI: 10.1002/adsc.201401000
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Automatic Pseudorotaxane Formation Targeting on Nucleic Acids Using a Pair of Reactive Oligodeox-ynucleotides (2014)
  • Author(s): Kazumitsu Onizuka*, Fumi Nagatsugi, Yoshihiro Ito, Hiroshi Abe
  • Journal Title: ournal of the American Chemical Society Volume: 136 Issue: 20 Pages: 7201-7204
  • DOI: 10.1021/ja5018283
  • Peer Reviewed
• [Journal Article] Synthesis of 3',4'-difluoro-3'-deoxyribonucleosides and its evaluation of the biological activities: Discovery of a novel type of anti-HCV agent 3',4'-difluorocordycepin (2014)
  • Author(s): Shimada H, Haraguchi K, Hotta K, Miyaike T, Kitagawa Y, Tanaka H, Kaneda R, Abe H, Shuto S, Mori K, Ueda Y, Kato N, Snoeck R, Andrei G, Balzarini J.
  • Journal Title: Bioorg. Med. Chem. Volume: 22 Issue: 21 Pages: 6174-82
  • DOI: 10.1016/j.bmc.2014.08.024
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] An intracellular buildup reaction of active siRNA species from short RNA fragments. (2014)
  • Author(s): Maruyama, H.; Nakashima, Y.; Shuto, S.; Matsuda, A.; Itoh, Y.; Abe, H.
  • Journal Title: Chem. Commun. Volume: 50 Issue: 11 Pages: 1284-1287
  • DOI: 10.1039/c3cc47529h
  • Peer Reviewed / Open Access
• [Journal Article] Rolling circle amplification in prokaryotic translation system using small circular RNA. (2013)
  • Author(s): Abe, N., Hiroshima, M., Maruyama, H., Nakashima, Y., Nakano, Y., Matsuda, A., Sako, Y., Ito, Y., and Abe, H.
  • Journal Title: Angew. Chem. Int. Ed. Volume: 52 Issue: 27 Pages: 7004-7008
  • DOI: 10.1002/anie.201302044
  • Peer Reviewed
• [Journal Article] Sustained delivery of siRNA from dopamine-coated stainless steel surface (2013)
  • Author(s): 10. Binata Joddar*, Aydin Albayrak, Jeonghwa Kang, Mizuki Nishihara, Hiroshi Abe, Yoshihiro Ito
  • Journal Title: Acta Biomaterialia Volume: 9 Pages: 6753-6761
  • Peer Reviewed
• [Journal Article] Cationic cholesterol-modified gelatin as an in vitro siRNA delivery vehicle (2013)
  • Author(s): P. A. Kadengodlu
  • Journal Title: Mol. BioSyst. Volume: 9 Issue: 5 Pages: 965-968
  • DOI: 10.1039/c2mb25424g
  • Peer Reviewed
• [Presentation] 人工核酸によるRNA機能の解析と制御 (2016)
  • Author(s): 阿部洋
  • Organizer: 2015年度東海高分子学生研究会
  • Place of Presentation: 名古屋
  • Year and Date: 2016-03-12
  • Invited
• [Presentation] 合成環状RNAからのタンパク質発現 (2015)
  • Author(s): 阿部洋，阿部奈保子, 伊藤嘉浩, 松本健, 吉田稔
  • Organizer: 日本核酸医薬学会第1回年会
  • Place of Presentation: 京都
  • Year and Date: 2015-11-30
• [Presentation] 細胞内RNAの解析と機能制御 (2015)
  • Author(s): 阿部洋
  • Organizer: 特別招待セミナー(愛知がんセンター)
  • Place of Presentation: 愛知がんセンター
  • Year and Date: 2015-11-20
  • Invited
• [Presentation] 低分子RNAから活性型高分子RNAを細胞内でビルドアップする方法論の開発 (2015)
  • Author(s): 阿部 洋、丸山 豪斗、周東 智、松田 彰
  • Organizer: RNA meeting 2015 in Sapporo
  • Place of Presentation: 札幌
  • Year and Date: 2015-07-15
• [Presentation] 人工核酸を用いた細胞内RNAの解析及び機能制御 (2015)
  • Author(s): 阿部洋
  • Organizer: 第一回新しい原子分子組織化物質・材料創出に向けた光・量子ビーム応用技術 調査専門委員会
  • Place of Presentation: 名古屋大学
  • Year and Date: 2015-05-08
  • Invited
• [Presentation] 標的を触媒として利用する遺伝子検出のための化学反応プローブの開発 (2014)
  • Author(s): 阿部洋
  • Organizer: 若手研究者のための有機化学札幌セミナー
  • Place of Presentation: 北海道大学理学部5号館
  • Year and Date: 2014-11-17
  • Invited
• [Presentation] DNA templated reaction for efficient signal amplification and its steady-state kinetic analysis of the turnover cycle (2014)
  • Author(s): Hiroshi Abe, Aya Shibata, Takanori Uzawa, Yoshihiro Ito, Satoshi Shuto
  • Organizer: The 41st International Symposium on Nucleic Acids Chemistry
  • Place of Presentation: Kitakyushu-shi
  • Year and Date: 2014-11-06
• [Presentation] Synthetic biology of Circular RNA (2014)
  • Author(s): Hiroshi Abe
  • Organizer: RIKEN SYPOSIUM & 15th Tokyo RNA club
  • Place of Presentation: Wako-shi
  • Year and Date: 2014-09-15
  • Invited
• [Presentation] RNAのナノ構造化に基づく機能創発 (2014)
  • Author(s): 阿部洋
  • Organizer: RNAインフォマティクス道場in 札幌
  • Place of Presentation: 産総研北海道センター
  • Year and Date: 2014-08-27
  • Invited
• [Presentation] RNAナノ構造化による医薬機能創発 (2014)
  • Author(s): 阿部洋
  • Organizer: 第25回 未来創薬・医療イノベーションセミナー
  • Place of Presentation: 北海道大学、札幌市
  • Year and Date: 2014-07-09
  • Invited
• [Patent(Industrial Property Rights)] 細胞内ビルドアップ型RNA (2013)
  • Inventor(s): 阿部洋
  • Industrial Property Rights Holder: 阿部洋
  • Industrial Property Rights Type: 特許
  • Filing Date: 2013-05-01
  • Overseas