| Project/Area Number |
25286029
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Nanobioscience
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| Research Institution | Tokyo University of Agriculture and Technology |
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Principal Investigator |
Norihiro Umeda 東京農工大学, 工学(系)研究科(研究院), 教授 (60111803)
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| Co-Investigator(Kenkyū-buntansha) |
OHTA Yoshihiro 東京農工大学, 大学院工学研究院, 准教授 (10223843)
IWAMI Kentaro 東京農工大学, 大学院工学研究院, 准教授 (80514710)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥19,760,000 (Direct Cost: ¥15,200,000、Indirect Cost: ¥4,560,000)
Fiscal Year 2015: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2013: ¥9,620,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥2,220,000)
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| Keywords | ミトコンドリア / ベント型光ファイバー / 局所微小荷重 / 蛍光色素 / 共焦点顕微鏡 / ヤング率 / ナノニュートン / ROS / 局所微小力 / オルガネラ / 活性酸素 |
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| Outline of Final Research Achievements |
Mitochondria produce reactive oxygen spices (ROS). Especially, in the case of ischemia- reperfusion injury, physical stress induced by blood flow at narrowing blood vessel causes ROS production from mitochondria in the living cell. However, the relationship between physical stress applied to mitochondrion and mitochondrial ROS production is absolutely unknown. To investigate this relationship, we developed a force-applying confocal laser scanning microscopy (F-CLSM). The ROS response of mitochondrion was measured by using MitoSOX fluorescent indicator. We confirmed 90 % difference of fluorescence increase rates between inhibitor and activator injections. Finally, we monitored the fluorescence of MitoSOX while applying force onto single mitochondrion. In the case of applied force of 40 nN, fluorescence intensity rate was larger than that of 10 nN. Therefore, we revealed that the ROS produced in the mitochondrion depends on the magnitude of physical stress applied to mitochondrion.
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