| Budget Amount *help |
¥14,430,000 (Direct Cost: ¥11,100,000、Indirect Cost: ¥3,330,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2013: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
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| Outline of Final Research Achievements |
The author have applied the latest method of theoretical calculations and graphene substrate to the base identification method (Nature sister paper) by DNA extension and fixation method (established patent, developed by the author) and scanning tunneling microscope (STM). By optimizing experimental conditions such as adoption, the author aimed at single molecule sequencing of all four kinds of bases. As a result, the author succeeded in sequencing not only guanine base but also adenine base. It is an important result as it is possible to know all four kinds of base molecules as complementarity of the DNA base only by sequencing adenine base and guanine base of one double stranded DNA.
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