Single molecule sequencing using graphene
| Project/Area Number |
25286031
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Nanobioscience
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| Research Institution | Osaka University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥14,430,000 (Direct Cost: ¥11,100,000、Indirect Cost: ¥3,330,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2013: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
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| Keywords | グラフェン / DNA / 1分子 / 可視化 / 顕微鏡 / STM / AFM / シークエンシング / 基板 / 走査プローブ顕微鏡 / ナノバイオ / 表面・界面物性 / 核酸 / ゲノム / SPM / ニッケル / 白金 / 薄膜 / 表面 / 真空 |
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| Outline of Final Research Achievements |
The author have applied the latest method of theoretical calculations and graphene substrate to the base identification method (Nature sister paper) by DNA extension and fixation method (established patent, developed by the author) and scanning tunneling microscope (STM). By optimizing experimental conditions such as adoption, the author aimed at single molecule sequencing of all four kinds of bases. As a result, the author succeeded in sequencing not only guanine base but also adenine base. It is an important result as it is possible to know all four kinds of base molecules as complementarity of the DNA base only by sequencing adenine base and guanine base of one double stranded DNA.
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Report
(6 results)
Research Products
(26 results)
