Study of sensitive immunosensor with the ZnO-coated plasmonic chip
| Project/Area Number |
25286032
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Nanobioscience
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| Research Institution | Kwansei Gakuin University (2016)
National Institute of Advanced Industrial Science and Technology (2013-2015) |
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Principal Investigator |
TAWA Keiko 関西学院大学, 理工学部, 教授 (80344109)
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| Co-Investigator(Kenkyū-buntansha) |
梅津 光央 東北大学, 工学(系)研究科(研究院), 教授 (70333846)
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000)
Fiscal Year 2015: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2014: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2013: ¥10,400,000 (Direct Cost: ¥8,000,000、Indirect Cost: ¥2,400,000)
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| Keywords | プラズモン / 格子 / サンドイッチアッセイ / IL-6 / 蛍光 / 酸化亜鉛 / プラズモン共鳴 / 増強蛍光 / バイオセンサー / 二重特性抗体 / イムノアッセイ / 二重特異性抗体 / 高感度検出 / イムノセンサー / 表面プラズモン共鳴 |
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| Outline of Final Research Achievements |
Interleukin-6（IL-6), a cytokine relating inframmatory and autoimmune activity, was detected with fluorescence assays using a plasmonic chip. In their assays, the way of surface modification, sample volume, incubation time and mixing solution, were found to influence the detection sensitivity. When the assay was revised in the point of a rapid and easy process, the detection sensitivity was not compromised compared to assays with sufficient sample volume and assay
time. In the assay with the ZnO-plasmonic chip and bispecific antibody, the limit of detection was found to be pM level for 10 microL of sample solution and 20 min of assay time.
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Report
(5 results)
Research Products
(48 results)
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[Journal Article] Rapid and Sensitive Detection of Brain-Derived Neurotrophic Factor with a Plasmonic Chip2013
Author(s)
Keiko Tawa, Mari Satoh, Koichi Uegaki, Tomoko Hara, Masami Kojima, Hiroyuki Aota, Yoshiki Yokota, Takahiko Nakaoki, Mitsuo Umetsu, Hikaru Nakazawa, Izumi Kumagai
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Journal Title
Japanese Journal of Applied Physics
Volume: 52
Issue: 6S
Pages: 06GK01-06GK01
DOI
NAID
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Peer Reviewed
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[Presentation] 酸化亜鉛コーティングプラズモニックチップ上のサンドイッチアッセイによるうつ病関連マーカーＢＤＮＦの迅速検出
Author(s)
M. Satoh, K. Tawa, K. Uegaki, T. Hara, M. Umetsu, H. Nakazawa, I. Kumagai, M. Itakura, M. Takahashi, H. Aota, M. Kojima
Organizer
高分子学会年次大会
Place of Presentation
京都国際会館
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