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Development of a fotonic microfluidic device for single cell analysis

Research Project

Project/Area Number 25286034
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Nano/Microsystems
Research InstitutionUniversity of Tsukuba

Principal Investigator

SUZUKI HIROAKI  筑波大学, 数理物質系, 教授 (20282337)

Co-Investigator(Kenkyū-buntansha) ASAKAWA KIYOSHI  筑波大学, 数理物質系, 客員教授 (20375405)
SUGIMOTO YOSHIMASA  独立行政法人物質・材料研究機構, 先端的共通技術部門, 主席研究員 (60415784)
OZAKI NOBUHIKO  和歌山大学, システム工学部, 准教授 (30344873)
YOKOKAWA MASATOSHI  筑波大学, 数理物質系, 助教 (50447885)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥19,240,000 (Direct Cost: ¥14,800,000、Indirect Cost: ¥4,440,000)
Fiscal Year 2015: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2014: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2013: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
Keywords細胞 / 光方向性結合器 / 導波路 / ポリマー / 窒化シリコン / バイオセンサ / 方向性結合器 / バイオセンシング / 微小流路 / 屈折率 / DNA / 単一細胞解析 / 方向性結合器干渉計 / マルチモード型干渉計 / サイトカイン / 抗体
Outline of Final Research Achievements

To detect molecules secreted from animal cells, an optical directional coupler (DC) was used to realize a micro biosensing system. First, the DC was fabricated with polymer waveguides and the function was confirmed. Then, a silicon nitride DC was fabricated. Simulation was conducted to design these devices and the performance of the devices was characterized. The device was coupled with a microfluidic system and sensing of biomolecules was conducted there.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • Research Products

    (10 results)

All 2016 2015 2014 Other

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Acknowledgement Compliant: 1 results) Presentation (9 results) (of which Int'l Joint Research: 4 results)

  • [Journal Article] Micron scale directional coupler as a transducer for biochemical sensing2015

    • Author(s)
      K. Uchiyamada, K. Okubo, M. Yokokawa, E. T. Carlen, K. Asakawa, and H. Suzuki
    • Journal Title

      Opt. Express

      Volume: 23 Issue: 13 Pages: 17156-17168

    • DOI

      10.1364/oe.23.017156

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] Fabrication and characterization of silicon nitride directional coupler interferometer for sensing aptamer hybridization2016

    • Author(s)
      Kyohei Okubo, Ken Uchiyamada, Kiyoshi Asakawa, Masatoshi Yokokawa, Hiroaki Suzuki
    • Organizer
      SPIE Photonics West 2016
    • Place of Presentation
      San Francisco, California, USA
    • Year and Date
      2016-02-14
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Micro-optofluidic ELISA for rapid cytokine detection toward drug susceptibility test2015

    • Author(s)
      Ryo Usuba, Masatoshi Yokokawa, Andreu Llobera, Soichiro Murata, Nobuhiro Ohkohchi, Hiroaki Suzuki
    • Organizer
      The 2015 International Chemical Congress of Pacific Basin Societies (PACIFICHEM)
    • Place of Presentation
      Honolulu, Hawaii, USA
    • Year and Date
      2015-12-16
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 血中サイトカイン定量による迅速診断に向けたオンチップELISA2015

    • Author(s)
      薄葉亮、横川雅俊、Andreu Llobera、村田聡一郎、大河内信弘、鈴木博章
    • Organizer
      第32回「センサ・マイクロマシンと応用システム」シンポジウム
    • Place of Presentation
      朱鷺メッセ(新潟県新潟市)
    • Year and Date
      2015-10-28
    • Related Report
      2015 Annual Research Report
  • [Presentation] Integrated Optofluidic Device for the Measurement of the Activity of Lymphocytes2015

    • Author(s)
      R. Usuba, M. Yokokawa, A. Llobera, S. Murata, N. Ohkohchi, H. Suzuki
    • Organizer
      IEEE Transducers 2015
    • Place of Presentation
      Anchorage, USA
    • Year and Date
      2015-06-23
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Directional-Coupler Interferometer Realizes a Miniaturized and High Sensitive Biosensor2015

    • Author(s)
      K. Uchiyamada, K. Okubo, M. Yokokawa, Edwin T. Carlen, K. Asakawa, H. Suzuki
    • Organizer
      2015 OSA Biomedical Optics & Photonics Congress: Optics in the Life Sciences
    • Place of Presentation
      Vancouver, Canada
    • Year and Date
      2015-04-15
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 光方向性結合型化学センサによる表面吸着物質の検出2015

    • Author(s)
      内山田健、大久保喬平、横川雅俊、Edwin E. Carlen、浅川潔、鈴木博章
    • Organizer
      電気化学会第82回春季講演会
    • Place of Presentation
      横浜国立大学
    • Year and Date
      2015-03-17
    • Related Report
      2014 Annual Research Report
  • [Presentation] 光干渉導波路型微小化学分析デバイスの周辺屈折率に対するシグナル応答評価2014

    • Author(s)
      内山田健、大久保喬平、横川雅俊、浅川潔、鈴木博章
    • Organizer
      応用物理学会第75回秋季学術講演会
    • Place of Presentation
      北海道大学
    • Year and Date
      2014-09-20
    • Related Report
      2014 Annual Research Report
  • [Presentation] 方向性結合型光導波路を用いた微小化学分析デバイスの作製

    • Author(s)
      内山田健、大久保喬平、横川雅俊、浅川潔、鈴木博章
    • Organizer
      応用物理学会第61回春季学術講演会
    • Place of Presentation
      青山学院大学(神奈川県)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 方向性結合器による化学センシング

    • Author(s)
      大久保喬平、内山田健、浅川潔、横川雅俊、鈴木博章
    • Organizer
      電気化学会第81回大会
    • Place of Presentation
      関西大学(大阪府)
    • Related Report
      2013 Annual Research Report

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Published: 2013-05-21   Modified: 2019-07-29  

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