| Budget Amount *help |
¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
Fiscal Year 2015: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
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| Outline of Final Research Achievements |
We recently developed frequency-modulation atomic force microscopy (FM-AFM) in liquids and made it possible to explore solid-liquid interface properties such as the hydration structures and surface charges with a nanometer-scale resolution. In this project, we employed FM-AFM to study the hydration structures and surface charges of biomolecules such as DNA by using two-dimensional (2D) and three-dimensional (3D) force mapping techniques. We obtained a 3D frequency shift map on a plasmid DNA molecule in a buffer solution with 50 mM nickel chloride. The map showed complicated contrast patterns with a sub-nanometer-scale resolution, which reflect the hydration structures around the DNA molecule. We also obtained a 3D frequency shift map on a lambda DNA molecule adsorbed on a poly-L-lysine (PL) layer on a mica substrate in potassium chloride solutions. By analyzing the frequency shift versus distance curves, we estimated the charge density of the labmda DNA molecule and the PL layer.
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