Localized surface plasmon sensing for subtype determination of influenza viruses in a specimen with ultra-small volume
| Project/Area Number |
25286062
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Optical engineering, Photon science
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| Research Institution | Muroran Institute of Technology |
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Principal Investigator |
KANO Hiroshi 室蘭工業大学, 工学(系)研究科(研究院), 准教授 (80322874)
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| Co-Investigator(Kenkyū-buntansha) |
HASEGAWA Hideki 国立感染症研究所, 感染病理部, 部長 (30301790)
MORIGAKI Kenichi 神戸大学, 遺伝子実験センター, 准教授 (10358179)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥19,110,000 (Direct Cost: ¥14,700,000、Indirect Cost: ¥4,410,000)
Fiscal Year 2015: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2014: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2013: ¥13,780,000 (Direct Cost: ¥10,600,000、Indirect Cost: ¥3,180,000)
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| Keywords | 表面プラズモン / 屈折率測定 / インフルエンザウイルス / サブタイプ判定 / 顕微屈折率計測 / 基板表面化学修飾 / ウイルス検出 |
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| Outline of Final Research Achievements |
Development of a method for quick subtype determination of influenza viruses is important because infectious and pathogenic properties of viruses are different. In this research, we proposed a method in which refractive index change due to antigen-antibody reaction between target viruses with certain subtype and monoclonal antibodies fixed in a micro-channel on a sensing substrate. To realize this concept, we successfully improved stability and sensitivity of a measurement system for virus detection, confirmed a measurement principle for faster data acquisition, and developed a technique to modify chemical property of a substrate surface to locate certain antibodies in a controlled region.
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Report
(4 results)
Research Products
(40 results)
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[Journal Article] Relationship of the quaternary structure of human secretory IgA to neutralization of influenza virus.2015
Author(s)
Suzuki T, Kawaguchi A, Ainai A, Tamura S, Ito R, Multihartina P, Setiawaty V, Pangesti KN, Odagiri T, Tashiro M, Hasegawa H.
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Journal Title
Proc Natl Acad Sci U S A.
Volume: 112(25)
Issue: 25
Pages: 7809-14
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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