Spatio-temporal analysis of cell mechanical properties and force propagations by atomic force microscopy

• Project/Area Number: 25286081
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: General applied physics
• Research Institution: Hokkaido University
• Principal Investigator: OKAJIMA TAKAHARU 北海道大学, 情報科学研究科, 教授 (70280998)
• Co-Investigator(Renkei-kenkyūsha): SUEOKA KAZUHISA 北海道大学, 情報科学研究科, 教授 (60250479)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000); Fiscal Year 2015: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2014: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2013: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000)
• Keywords: 原子間力顕微鏡 / 細胞力学 / 細胞レオロジー / 細胞骨格 / 生物物理

Outline of Final Research Achievements

Cytoskeletal (CSK) filaments and nuclear scaffolds are discretely connected to each other in response to external forces. The force propagation plays a role in regulating various cell functions. The understanding of cell mechanical properties related to the CSK filamentous structures is essential to elucidate the mechanisms of cell functions. We fabricated micro-fabricated substrate for controlling cell shape and observing intracellular structures with optical microscope. For the investigation of intra-and intercellular mechanical properties associated with CSK filaments, we developed atomic force microscopy (AFM) that allows us to measure the mechanical properties of cell population in an extremely large region and to map rapidly the cell rheological properties, We investigated the correlation between cell rheology and CSK, the emerging spatial distribution of cell modulus in cell population, the motion of cell nucleus related to CSK, and the temporal change in cell rheology.

Research Products

• [Journal Article] Mapping power-law rheology of living cells using multi-frequency force modulation atomic force microscopy (2015)
  • Author(s): R. Takahashi, T. Okajima
  • Journal Title: Applied Physics Letters Volume: 107 Issue: 17 Pages: 173702-173702
  • DOI: 10.1063/1.4934874
  • NAID: 120005677755
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Time-lapse imaging of morphological changes in a single neuron during the early stages of apoptosis using scanning ion conductance microscopy (2015)
  • Author(s): A. Tanaka*, R. Tanaka, N. Kasai, S. Tsukada, T. Okajima, K. Sumitomo
  • Journal Title: Journal of Structural Biology Volume: 191 Issue: 1 Pages: 32-38
  • DOI: 10.1016/j.jsb.2015.06.002
  • Peer Reviewed
• [Journal Article] Precision of cell-to-cell variation in power-law rheology characterized by atomic force microscopy (2015)
  • Author(s): P.G. Cai, T. Okajima
  • Journal Title: Japanese Journal of Applied Physics Volume: 54 Issue: 3 Pages: 037001-037001
  • DOI: 10.7567/jjap.54.037001
  • NAID: 210000144874
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Filamin acts as a key regulator in epithelial defence against transformed cells. (2014)
  • Author(s): Kajita M., Sugimura, K., Ohoka, A., Burden, J., Suganuma, H., Ikegawa, M., Shimada, T., Kitamura, T., Shindoh, M., Ishikawa, S., Yamamoto, S., Saitoh, S., Yako, Y., Takahashi, R., Okajima, T., Kikuta, J., Maijima, Y., Ishii, M., Tada, M., and Fujita, Y.
  • Journal Title: Nature Communications Volume: 5 Issue: 1 Pages: 4428-4428
  • DOI: 10.1038/ncomms5428
  • Peer Reviewed / Open Access
• [Journal Article] Atomic force microscopy measurements of mechanical properties of single cells patterned by microcontact printing (2014)
  • Author(s): R.Takahashi, S. Ichikawa, A. Subagyo, K. Sueoka, and T. Okajima*
  • Journal Title: Advanced Robotics Volume: 28 Issue: 7 Pages: 449-455
  • DOI: 10.1080/01691864.2013.876933
  • Peer Reviewed
• [Journal Article] 原子間力顕微鏡による細胞力学の定量計測（解説） (2014)
  • Author(s): 岡嶋孝治，
  • Journal Title: 検査技術 Volume: 3 Pages: 1-5
• [Journal Article] 走査プローブ顕微鏡による細胞物性測定：原子間力顕微鏡とイオンコンダクタンス顕微鏡（総説） (2013)
  • Author(s): 岡嶋孝治
  • Journal Title: 化学工業 Volume: 64( Pages: 612-617
• [Presentation] Quantifying local elastic modulus of living embryo by atomic force microscopy (2015)
  • Author(s): Y. Fujii*, W. Koizumi, K. Hotta, K. Oka, T. Okajima
  • Organizer: 23rd International Colloquium on Scanning Probe Microscopy (ICSPM23)
  • Place of Presentation: ニセコ（ヒルトンホテル）
  • Year and Date: 2015-12-10
  • Int'l Joint Research
• [Presentation] Multi-frequency force modulation atomic force microscopy:Simultaneous measurements of living cells in frequency and time domains (2015)
  • Author(s): R. Takahashi*, T. Okajima,
  • Organizer: 23rd International Colloquium on Scanning Probe Microscopy (ICSPM23)
  • Place of Presentation: ニセコ（ヒルトンホテル）
  • Year and Date: 2015-12-10
  • Int'l Joint Research
• [Presentation] Mechanical Measurements of in vitro Tissue by Atomic Force Microscopy (2015)
  • Author(s): Ryosuke Tanaka*, Takaharu Okajima
  • Organizer: 26th 2015 International Symposium on Micro-NanoMechatronics and Human Science
  • Place of Presentation: 名古屋（名古屋大学）
  • Year and Date: 2015-11-23
  • Int'l Joint Research
• [Presentation] 原子間力顕微鏡による肝細胞共培養系の力学特性解析 (2015)
  • Author(s): 田中良昌*、秋山義勝、小林純、大和雅之、岡嶋孝治
  • Organizer: 第53回生物物理学会
  • Place of Presentation: 金沢（金沢大学）
  • Year and Date: 2015-09-13
• [Presentation] 原子間力顕微鏡によるホヤ初期発生胚の弾性率の時空間測定 (2015)
  • Author(s): 藤井裕紀*、小泉航、堀田耕司、岡浩太郎、岡嶋孝治
  • Organizer: 第53回生物物理学会
  • Place of Presentation: 金沢（金沢大学）
  • Year and Date: 2015-09-13
• [Presentation] 原子間力顕微鏡による力学的単一細胞診断：細胞力学量のばらつきの空間依存性 (2015)
  • Author(s): 澤野麻紀*、繁富（栗林）香織、朱きん峰、高橋亮輔、スバギョ・アグス、末岡和久、岡嶋孝治
  • Organizer: 第53回生物物理学会
  • Place of Presentation: 金沢（金沢大学）
  • Year and Date: 2015-09-13
• [Presentation] 原子間力顕微鏡によるマウス顎下腺組織の弾性率マッピング (2015)
  • Author(s): 中島優*、中村光宏、武田宏明、松本卓也、岡嶋孝治、
  • Organizer: 第53回生物物理学会
  • Place of Presentation: 金沢（金沢大学）
  • Year and Date: 2015-09-13
• [Presentation] 原子間力顕微鏡：細胞力学特性の個性を測る (2015)
  • Author(s): 岡嶋孝治
  • Organizer: 第53回生物物理学会
  • Place of Presentation: 金沢（金沢大学）
  • Year and Date: 2015-09-13
  • Invited
• [Presentation] Spatial-Temporal Oscillation in Elastic Modulus of Embryo during the Early Development (2015)
  • Author(s): Yuki Fujii*, Wataru Koizumi, Koji Hotta and Takaharu Okajima,
  • Organizer: International Symposium on Fluctuation and Structure Out of Equilibrium 2015 (SFS2015)
  • Place of Presentation: 京都（京都大学）
  • Year and Date: 2015-08-21
• [Presentation] 細胞シートの延伸による細胞核の形状変化 (2015)
  • Author(s): 大西恒太*，土屋雅博，岡嶋孝治
  • Organizer: 第67回細胞生物学会
  • Place of Presentation: 船堀（タワーホール船橋）
  • Year and Date: 2015-06-30
• [Presentation] 単層上皮細胞における細胞弾性率の空間不均一構造 (2015)
  • Author(s): 藤井裕紀*，越智勇貴，梶田美穂子，藤田恭之，岡嶋孝治
  • Organizer: 第67回細胞生物学会
  • Place of Presentation: 船堀（タワーホール船橋）
  • Year and Date: 2015-06-30
• [Presentation] 原子間力顕微鏡による力学的単一細胞診断法の開発：細胞力学と細胞骨格構造の相関 (2015)
  • Author(s): 澤野麻紀*，繁富香織，朱キン峰，高橋亮輔，スバギョアグス，末岡和久，田中良昌，岡嶋孝治
  • Organizer: 第67回細胞生物学会
  • Place of Presentation: 船堀（タワーホール船橋）
  • Year and Date: 2015-06-30
• [Presentation] 原子間力顕微鏡によるマウス顎下腺の弾性率測定 (2015)
  • Author(s): 中島優*，中村光宏，武田宏明，松本卓也，岡嶋孝治
  • Organizer: 第67回細胞生物学会
  • Place of Presentation: 船堀（タワーホール船橋）
  • Year and Date: 2015-06-30
• [Presentation] 細胞の物理学：走査プローブ顕微鏡によるアプローチ (2015)
  • Author(s): 岡嶋孝治
  • Organizer: 表面科学会東北北海道支部学術研究会
  • Place of Presentation: 札幌
  • Year and Date: 2015-03-09 – 2015-03-10
  • Invited
• [Presentation] Origin of cell-to-cell mechanical variation investigated by atomic force microscopy (2014)
  • Author(s): P.G. Cai*, T. Okajima
  • Organizer: 22nd International Colloquium on Scanning Probe Microscopy (ICSPM22)
  • Place of Presentation: Shizuoka
  • Year and Date: 2014-12-11 – 2014-12-13
• [Presentation] 細胞レオロジーの時間変動と空間分布：原子間力顕微鏡測定 (2014)
  • Author(s): 岡嶋孝治、蔡萍根、高橋亮輔、繁富（栗林）香織
  • Organizer: バイオオプティクス研究会
  • Place of Presentation: 大阪
  • Year and Date: 2014-12-05 – 2014-12-06
  • Invited
• [Presentation] Quantitative rheological measurements of confluent cell using atomic force microscopy (2014)
  • Author(s): R. Takahashi*, K. Kuribayashi-Shigetomi, A. Subagyo, K. Sueoka, T. Okajima
  • Organizer: 25th 2014 International Symposium on Micro-NanoMechatronics and Human Science
  • Place of Presentation: Nagoya
  • Year and Date: 2014-11-09 – 2014-11-12
• [Presentation] Temporal and Spatial Variations of Single Cell Rheology Investigated by Atomic Force Microscopy (2014)
  • Author(s): P.G. Cai*, R. Takahashi, K. Kuribayashi-Shigetomi, A. Subagyo, K. Sueoka, T. Okajima
  • Organizer: 25th 2014 International Symposium on Micro-NanoMechatronics and Human Science
  • Place of Presentation: Nagoya
  • Year and Date: 2014-11-09 – 2014-11-12
• [Presentation] 原子間力顕微鏡による細胞レオロジー測定 (2014)
  • Author(s): 岡嶋孝治
  • Organizer: 第34回表面科学学術講演会
  • Place of Presentation: 島根
  • Year and Date: 2014-11-06 – 2014-11-08
  • Invited
• [Presentation] 多重周波数モジュレーション原子間力顕微鏡：単一細胞レオロジーの高速測定 (2014)
  • Author(s): 高橋亮輔*，繁富(栗林)香織、スバギョ・アグス，末岡和久，岡嶋孝治
  • Organizer: 第52回日本生物物理学会年会
  • Place of Presentation: 札幌
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] マイクロパターン上に培養した単一細胞の細胞核の動態 (2014)
  • Author(s): 朱きん峰*, 繁富（栗林）香織, 蔡萍根, スバギョ・アグス, 末岡和久，岡嶋孝治
  • Organizer: 第52回日本生物物理学会年会
  • Place of Presentation: 札幌
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] 細胞接着基板延伸装置を用いた細胞動態のタイムラプス測定 (2014)
  • Author(s): 武藏湧貴*，土屋雅博, 坂井譲, 朱きん峰, 岡嶋孝治
  • Organizer: 第75回応用物理学会秋期学術講演会
  • Place of Presentation: 札幌
  • Year and Date: 2014-09-17 – 2014-09-20
• [Presentation] 細胞蛍光顕微鏡観察が可能な牽引力の評価 (2014)
  • Author(s): 坂井譲*, 武蔵湧貴，朱きん峰，土屋雅博，岡嶋孝治
  • Organizer: 第75回応用物理学会秋期学術講演会
  • Place of Presentation: 札幌
  • Year and Date: 2014-09-17 – 2014-09-20
• [Presentation] 原子間力顕微鏡による単層上皮細胞の粘弾性の空間不均一性 (2014)
  • Author(s): 藤井裕紀*，越智勇貴，岡嶋孝治
  • Organizer: 第66回日本細胞生物学会
  • Place of Presentation: 奈良
  • Year and Date: 2014-06-11 – 2014-06-13
• [Presentation] マイクロパターン基板を用いた細胞核形状の測定と細胞周期依存性 (2013)
  • Author(s): 黒澤亮*, 繁富香織, アグス・スバギョ, 末岡和久, 岡嶋孝治
  • Organizer: 第３６回日本分子生物学会
  • Place of Presentation: 神戸（神戸国際会議場）
• [Presentation] 細胞間力学変化量の空間不均一性：原子間力顕微鏡測定 (2013)
  • Author(s): 高橋亮輔*，繁富(栗林）香織，岡嶋孝治
  • Organizer: 第51回日本生物物理学会年会
  • Place of Presentation: 京都（国立京都国際会館）
• [Book] Nano/Micro Science and Technology in Biorheology: Principles, Methods, and Applications (2015)
  • Author(s): T. Okajima
  • Total Pages: 28
  • Publisher: Springer
• [Book] Hyper Bio Assembler for 3D Cellular Systems (2015)
  • Author(s): K. Kuribayashi-Shigetomi, R. Takahashi, A. Subagyo, K. Sueoka, T. Okajima
  • Total Pages: 8
  • Publisher: Springer
• [Book] 光学 (2015)
  • Author(s): 水谷祐輔，田中良昌，田中あや，住友弘二，岡嶋孝治
  • Total Pages: 6
  • Publisher: 日本光学会
• [Book] ケミカルエンジニヤリング (2015)
  • Author(s): 高橋亮輔、岡嶋孝治
  • Total Pages: 6
  • Publisher: 化学工業社
• [Book] 三次元ティッシュエンジニアリング～細胞の培養・操作・組織化から品質管理、脱細胞化まで～ (2015)
  • Author(s): 岡嶋孝治
  • Total Pages: 6
  • Publisher: 株式会社NTS
• [Patent(Industrial Property Rights)] 細胞の複素弾性率の計測方法および計測システム (2014)
  • Inventor(s): 岡嶋孝治、高橋亮輔
  • Industrial Property Rights Holder: 岡嶋孝治、高橋亮輔
  • Industrial Property Rights Type: 特許
  • Filing Date: 2014-07-02