Impact of the Solar constant to deep-sea microbial community

• Project/Area Number: 25287149
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Geochemistry/Cosmochemistry
• Research Institution: Japan Agency for Marine-Earth Science and Technology
• Principal Investigator: TAKANO Yoshinori 国立研究開発法人海洋研究開発機構, 生物地球化学研究分野, 主任研究員 (80399815)
• Co-Investigator(Renkei-kenkyūsha): KANEKO Masanori 国立研究開発法人産業技術総合研究所, 地圏資源環境研究部門, 主任研究員 (80633239)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥16,510,000 (Direct Cost: ¥12,700,000、Indirect Cost: ¥3,810,000); Fiscal Year 2015: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2014: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2013: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
• Keywords: 有機分子レベル解析・分子内レベル解析 / 海底下の地下生命圏 / 性状未知アーキアの生理生態 / ピルビン酸ファミリーアミノ酸族 / 液体クロマトグラフィー／質量分析法 / ガスクロマトグラフィー／質量分析法 / ガスクロマトグラフィー／同位体質量分析法 / ガスクロマトグラフィー/質量分析法 / ガスクロマトグラフィー/同位体質量分析法 / 有機分子 / 性状未知アーキア / 生理生態 / アミノ酸の窒素同位体比 / 従属栄養 / アミノ酸 / 代謝経路 / サルベージプロセス

Outline of Final Research Achievements

We conducted the method development using liquid chromatography and gas chromatography/ combustion/ isotope ratio mass spectrometry, occasionally coupled as LC x GC/C/IRMS to determine key molecules and their carbon/nitrogen isotopic compositions (e.g., lipids, amino acids, chlorophyll and other molecules). After the confirmation of high recovery during chromatographic separation without isotopic fractionation, we validated the accuracy and precision of the nitrogen isotopic composition of amino acids. To trace the metabolic pathways together with Enzyme Commission code, we customized the analytical scheme of the molecular sequencer including DNA and RNA pre-treatments.

Research Products

• [Int'l Joint Research] ドイツ連邦地質調査所/ブレーメン大学/マックスプランク研究所(ドイツ)
• [Journal Article] Estimation of methanogenesis by quantification of coenzyme F430 in marine sediments (2016)
  • Author(s): Kaneko, M., Takano, Y., Ogawa, N.O., Sato, Y., Yoshida, Y., Ohkouchi, N.
  • Journal Title: GEOCHEMICAL JOURNAL Volume: 50 Issue: 6 Pages: 453-453
  • DOI: 10.2343/geochemj.2.0410
  • NAID: 130005435143
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] A diatom inferred record of lake hydrological variability during the last 900 years in Lutzow-Holm Bay, East Antarctica. (2016)
  • Author(s): Rudd, R.C., Tyler, J.J., Tibby, J., Yokoyama, Y., Tavernier, I., Verleyen, E., Fukui, M. and Takano, Y.
  • Journal Title: Journal of Quaternary Science Volume: -
  • Peer Reviewed
• [Journal Article] Exploring deep microbial life in coal-bearing sediments down to ~2.5km below the oceanfloor (2015)
  • Author(s): INAGAKI FUMIO, Kai-Uwe Hinrichs,KUBO YUSUKE,Marshall W. Bowles,Verena B. Heuer,Wei-Li Hong,HOSHINO TATSUHIKO,IJIRI AKIRA,IMACHI HIROYUKI,ITO MOTOO,KANEKO MASANORI,Mark A. Lever,Lin Yu-Shin,Barbara A. Methe,Morita Sumito,MORONO YUKI,et al.
  • Journal Title: Science Volume: 349 Pages: 420-424
  • DOI: 10.1126/science.aaa6882
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Diet quality influences isotopic discrimination among amino acids in an aquatic vertebrate (2015)
  • Author(s): Chikaraishi, Y., Steffan, S., Takano, Y., Ohkouchi, N
  • Journal Title: Ecology and Evolution Volume: 5 Pages: 2048-2059
  • DOI: 10.1002/ece3.1491
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Biogeochemistry and limnology in Antarctic subglacial weathering: molecular evidence of the linkage between subglacial silica input and primary producers in a perennially ice-covered lake. (2015)
  • Author(s): Takano, Y., Kojima, H., Takeda, E., Yokoyama, Y., Fukui, M.
  • Journal Title: Progress in Earth and Planetary Science Volume: 2 Pages: 8-8
  • DOI: 10.1186/s40645-015-0036-7
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Isolation of underivatized amino acids by ion-pair high performance liquid chromatography for precise measurement of nitrogen isotopic composition of amino acids: development of comprehensive LC x GC/C/IRMS method. (2015)
  • Author(s): Takano, Y., Chikaraishi, Y. and Ohkouchi, N.
  • Journal Title: International Journal of Mass Spectrometry Volume: 379 Pages: 16-25
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] イオンペアクロマトグラフィー／電子スプレーイオン化質量分析法 (LC/ESI-MS)によるアミノ酸のマススペクトル解析 [技術論文]. (2015)
  • Author(s): 高野 淑識, 力石 嘉人, 大河内 直彦
  • Journal Title: Researches in Organic Geochemistry Volume: 31 Pages: 33-49
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Isolation of underivatized amino acids by ion-pair high performance liquid chromatography for precise measurement of nitrogen isotopic composition of amino acids: development of comprehensive LC x GC/C/IRMS method. (2015)
  • Author(s): Takano, Y., Chikaraishi, Y., Ohkouchi, N.
  • Journal Title: International Journal of Mass Spectrometry Volume: 379 Pages: 16-25
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] マントルゼノリスの炭素と窒素の起源について（依頼総説） (2015)
  • Author(s): 高野 淑識・大河内 直彦
  • Journal Title: 地学雑誌 Volume: -
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Quantitative Analysis of Coenzyme F430 in Environmental Samples: A New Diagnostic Tool for Methanogenesis and Anaerobic Methane Oxidation (2014)
  • Author(s): Kaneko, M., Takano, Y., Chikaraishi, Y., Ogawa, N.O., Asakawa, S., Watanabe, K., Shima, S., Krueger, M., Matsushita, M., Kimura, H., Ohkouchi, N.
  • Journal Title: Analytical Chemistry Volume: 86 Pages: 3633-3638
  • DOI: 10.1021/ac500305j
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Organic nitrogen: sources, fates, and chemistry. (2014)
  • Author(s): Ohkouchi, N. and Takano, Y.
  • Journal Title: Treatise on Geochemistry, 10: Organic Geochemistry (Edited by Birrer, B., Falkowski, P., Freeman, K.) Volume: 12 Pages: 251-289
  • DOI: 10.1016/b978-0-08-095975-7.01015-9
  • Peer Reviewed
• [Journal Article] Detection of coenzyme F430 in deep sea sediments: A key molecule for biological methanogenesis (2013)
  • Author(s): Takano, Y., Kaneko, M., Kahnt, J., Imachi, H., Shima, S. and Ohkouchi, N.
  • Journal Title: Organic Geochemistry Volume: 58 Pages: 137-140
  • DOI: 10.1016/j.orggeochem.2013.01.012
  • Peer Reviewed
• [Journal Article] An interlaboratory study of TEX86 and BIT analysis of sediments, extracts and standard mixtures (2013)
  • Author(s): Schouten, S., Hopmans, E.C., Rosell-Mele, A., Pearson, A. et al. (including Takano, Y. and Ohkouchi, N.)
  • Journal Title: Geochemistry, Geophysics, Geosystems Volume: 14 Pages: 5263-5285
  • DOI: 10.1002/2013gc004904
  • Peer Reviewed
• [Journal Article] Diversity of sulfur-cycle prokaryotes in freshwater lake sediments investigated using aprA as functional marker gene (2013)
  • Author(s): Watanabe, T., Kojima, H., Takano, Y., and Fukui, M.
  • Journal Title: Systematics and Applied Microbiology Volume: 36 Pages: 436-443
  • DOI: 10.1016/j.syapm.2013.04.009
  • Peer Reviewed
• [Journal Article] Distribution and isotopic signatures of archaeal lipid biomarkers associated with gas hydrate occurrences on the northern Cascadia Margin. (2013)
  • Author(s): Kaneko, M., Naraoka, H., Takano, Y. and Ohkouchi, N.
  • Journal Title: Chemical Geology Volume: 343 Pages: 76-84
  • DOI: 10.1016/j.chemgeo.2013.02.003
  • Peer Reviewed
• [Presentation] Amino acids in deep-sea archaeal methanotrophy: new insight for lipid and methane biogeochemistry (2015)
  • Author(s): Yoshinori Takano, Yoshito Chikaraishi , Hiroyuki Imachi, Masanori Kaneko, Nanako O. Ogawa, Martin Kruger, and Naohiko Ohkouchi
  • Organizer: International Meeting on Organic Geochemistry
  • Place of Presentation: プラハ国際会議場（チェコ）
  • Year and Date: 2015-09-22
  • Int'l Joint Research
• [Presentation] How do microbes mediate the nitrogen cycle in the marine subseafloor? (2015)
  • Author(s): N. Ohkouchi, Y. Yamaguchi, Y. Chikaraishi, Y. Takano, N.O. Ogawa, H. Suga, and Y. Yokoyama
  • Organizer: International Meeting on Organic Geochemistry
  • Place of Presentation: プラハ国際会議場（チェコ）
  • Year and Date: 2015-09-02
  • Int'l Joint Research
• [Presentation] Carbon isotopic composition of Coenzyme F430 from anaerobic methane oxidizing archaea (2015)
  • Author(s): MASANORI KANEKO*1, YOSHINORI TAKANO2, NANAKO O. OGAWA2, NAOHIKO OHKOUCHI2
  • Organizer: Goldschmidt2015
  • Place of Presentation: プラハ国際会議場（チェコ）
  • Year and Date: 2015-08-18
  • Int'l Joint Research
• [Presentation] 海底下メタンの行方と地球生命科学 (2015)
  • Author(s): 高野 淑識・力石 嘉人・大河内 直彦
  • Organizer: JpGU2015
  • Place of Presentation: 幕張メッセ（千葉県千葉市）
  • Year and Date: 2015-05-25
  • Int'l Joint Research / Invited
• [Presentation] アミノ酸の窒素同位体比からみた植物の従属栄養 (2014)
  • Author(s): 力石嘉人・高野淑識・大河内直彦
  • Organizer: 2014年有機地球化学シンポジウム
  • Place of Presentation: ニューウエルシティ湯河原（熱海市）
  • Year and Date: 2014-11-05
• [Presentation] LC-MS 分析時の夾雑物の影響－F430 の定量・同位体分析のために (2014)
  • Author(s): 金子 雅紀・高野 淑識・小川 奈々子・大河内 直彦
  • Organizer: 2014年有機地球化学シンポジウム
  • Place of Presentation: ニューウエルシティ湯河原（熱海市）
  • Year and Date: 2014-11-05
• [Presentation] 南海トラフ地域におけるメタン生成補酵素F430 の分布 (2014)
  • Author(s): 佐藤友規・金子雅紀・高野淑識・大河内直彦・山田桂大・吉田尚弘
  • Organizer: 2014年有機地球化学シンポジウム
  • Place of Presentation: ニューウエルシティ湯河原（熱海市）
  • Year and Date: 2014-11-05
• [Presentation] C4植物の有機態窒素利用：アミノ酸の同位体比解析から得られた証拠 (2014)
  • Author(s): 力石嘉人，高野淑識，大河内直彦
  • Organizer: 2014年 日本地球化学会
  • Place of Presentation: 富山大学（富山市）
  • Year and Date: 2014-09-16
• [Presentation] 南極スカルブスネス露岩地域に分布する湖沼の堆積物のバイオマーカーに記録された古環境・古生態系変動 (2014)
  • Author(s): 竹田真佑美，沢田 健，高野 淑識
  • Organizer: 2014年 日本地球化学会
  • Place of Presentation: 富山大学（富山市）
  • Year and Date: 2014-09-16
• [Presentation] 有機分子から見えてくる性状未知アーキアの生理生態とメタンのダイナミクス (2014)
  • Author(s): 高野 淑識
  • Organizer: 2014年水環境学会シンポジウム
  • Place of Presentation: 滋賀県立大（彦根市）
  • Year and Date: 2014-09-09
  • Invited
• [Presentation] アミノ酸の安定同位体比を正確かつ感度良く測定するための分析法 (2014)
  • Author(s): 力石 嘉人, 高野 淑識, 大河内 直彦
  • Organizer: 2014年 日本地球惑星科学連合大会
  • Place of Presentation: パシフィコ横浜（横浜市）
  • Year and Date: 2014-05-01
• [Presentation] 海底下メタン生成研究への新しいアプローチ法 (2014)
  • Author(s): 金子 雅紀, 高野 淑識, 大河内 直彦
  • Organizer: 2014年 日本地球惑星科学連合大会
  • Place of Presentation: パシフィコ横浜（横浜市）
  • Year and Date: 2014-05-01
• [Presentation] 有機物から最大限の情報を引き出すために (2014)
  • Author(s): 大河内 直彦, 力石 嘉人, 高野 淑識, 小川 奈々子
  • Organizer: 2014年 日本地球惑星科学連合大会
  • Place of Presentation: パシフィコ横浜（横浜市）
  • Year and Date: 2014-05-01
• [Presentation] イオンペアクロマトグラフィー／電子スプレーイオン化質量分析法およびコロナCAD検出法の有機地球化学への応用（口頭発表） (2013)
  • Author(s): 高野 淑識, 力石 嘉人, 大河内 直彦
  • Organizer: 日本有機地球化学会
  • Place of Presentation: 岡山県・倉敷市（芸文館）
• [Presentation] Possible energetic linkage between primary production and deep-sea benthic archaea: insight from biogeochemical lipidomics（招待講演） (2013)
  • Author(s): Takano, Y. and Ohkouchi, N.
  • Organizer: EGU2013
  • Place of Presentation: オーストリア国・ウイーン（Wien Kaisermuhlen/VIC）
  • Invited
• [Book] バイオサイエンスシリーズ「アストロバイオロジー」（山岸 明彦 編） (2013)
  • Author(s): 高野 淑識・関根 康人（分担執筆）
  • Publisher: 化学同人
• [Remarks] 国立研究開発法人 海洋研究開発機構 生物地球化学研究分野
  • URL: http://www.jamstec.go.jp/biogeochem/
• [Remarks] 高野 淑識（主任研究員）
  • URL: http://www.jamstec.go.jp/res/ress/takano/
• [Remarks] 海洋研究開発機構 生物地球化学研究分野
  • URL: http://www.jamstec.go.jp/biogeochem/
• [Remarks] 海洋研究開発機構 生物地球化学研究分野
  • URL: http://www.jamstec.go.jp/biogeos/j/elhrp/biogeochem/