Development of systems for time-resolved intermolecular interaction detection during protein reactions and application to reaction mechanism
| Project/Area Number |
25288005
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Physical chemistry
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| Research Institution | Kyoto University |
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Principal Investigator |
Terazima Masahide 京都大学, 理学(系)研究科(研究院), 教授 (00188674)
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2015: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2013: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
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| Keywords | タンパク質 / 反応 / 時間分解 / 拡散係数 / 分子間相互作用 / タンパク質反応 / 光反応 / 拡散 / 熱力学 |
|---|
| Outline of Final Research Achievements |
In order to measure the translational diffusion coefficient change by the transient grating method combined with a stopped flow system, we constructed a new stopped flow system for minimizing turbulence after mixing two solutions. This system can suppress the turbulence within 100 ms after the mixing. Furthermore, we improved the system to decrease a volume of required sample solution, and succeeded in mixing only a few micro liter solution, which is a big advantage for application to protein samples. By using this system, we measured a diffusion coefficient change in time-domain after acid denaturation of a photosensor protein of phototropin sample. Moreover, association reaction of a protein of KaiC which has a function to control the circadian rhythm was detected in time-domain.
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Report
(5 results)
Research Products
(71 results)
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[Journal Article] Pressure-sensitive Reaction Yield of the TePixD Blue-light Sensor Protein.2015
Author(s)
Kuroi, K., Okajima, K., Ikeuchi, M., Tokutomi, S., Kamiyama, T. and Terazima, M.
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Journal Title
J. Phys. Chem
Volume: 119
Issue: 7
Pages: 2897-2907
DOI
NAID
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Peer Reviewed / Open Access
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[Book] 物性科学センター誌2016
Author(s)
中曽根祐介，寺嶋正秀
Total Pages
18
Publisher
京都大学環境安全保健機構 物性科学センター
Related Report
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[Book] 現代物理化学2015
Author(s)
寺嶋正秀, 馬場正昭, 松本吉泰
Total Pages
522
Publisher
化学同人
Related Report
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