Absolutely quantitative molecular imaging of living cells by Raman spectroscopy and development of physical chemistry toward life sciences
Project/Area Number |
25288010
|
Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Partial Multi-year Fund |
Section | 一般 |
Research Field |
Physical chemistry
|
Research Institution | Waseda University |
Principal Investigator |
Hamaguchi Hiro-o 早稲田大学, ナノ・ライフ創新研究機構, 教授 (00092297)
|
Co-Investigator(Kenkyū-buntansha) |
ISHIBASHI Taka-aki 筑波大学, 数理物質科学研究科, 教授 (70232337)
|
Project Period (FY) |
2013-04-01 – 2016-03-31
|
Project Status |
Completed (Fiscal Year 2015)
|
Budget Amount *help |
¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000)
Fiscal Year 2015: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2014: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
Fiscal Year 2013: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
|
Keywords | ラマン分光 / 分子イメージング / 絶対散乱断面積 / 生細胞 / ラマン散乱断面積 / 水素分子 / ベンゼン / 生体分子 |
Outline of Final Research Achievements |
We attempted to determine the absolute Raman cross sections of fundamental molecules in order to achieve absolutely quantitative molecular imaging of living cells. We first quantum-chemically calculated the Raman scattering tensor of the hydrogen molecule. The calculated tensor well reproduced the experimentally determined polarizability anisotropy and its first- and second derivatives with rtespect to the bond length. We use this theoretical Raman scattering tensor to calculate the absolute Raman cross sections of the pure rotationa Raman transitions to be used as the primary standard of the Raman intensity measurement. We measured Raman intensities of gaseous hydrogen and benzene vapor under the same experimental conditions and determined the absolute Raman cross section of benzene vapor. The ontained absolute Raman cross section agreed well with the literature values after the correction for the dielectric field effect.
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Report
(4 results)
Research Products
(16 results)
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[Book] ラマン分光法2015
Author(s)
濵口宏夫、岩田耕一
Total Pages
205
Publisher
講談社サイエンティフィク
Related Report
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[Book] ラマン分光法2015
Author(s)
濵口宏夫、岩田耕一共編著
Total Pages
205
Publisher
講談社サイエンティフィク
Related Report