Absolutely quantitative molecular imaging of living cells by Raman spectroscopy and development of physical chemistry toward life sciences

• Project/Area Number: 25288010
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Physical chemistry
• Research Institution: Waseda University
• Principal Investigator: Hamaguchi Hiro-o 早稲田大学, ナノ・ライフ創新研究機構, 教授 (00092297)
• Co-Investigator(Kenkyū-buntansha): ISHIBASHI Taka-aki 筑波大学, 数理物質科学研究科, 教授 (70232337)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000); Fiscal Year 2015: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2014: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000); Fiscal Year 2013: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
• Keywords: ラマン分光 / 分子イメージング / 絶対散乱断面積 / 生細胞 / ラマン散乱断面積 / 水素分子 / ベンゼン / 生体分子

Outline of Final Research Achievements

We attempted to determine the absolute Raman cross sections of fundamental molecules in order to achieve absolutely quantitative molecular imaging of living cells. We first quantum-chemically calculated the Raman scattering tensor of the hydrogen molecule. The calculated tensor well reproduced the experimentally determined polarizability anisotropy and its first- and second derivatives with rtespect to the bond length. We use this theoretical Raman scattering tensor to calculate the absolute Raman cross sections of the pure rotationa Raman transitions to be used as the primary standard of the Raman intensity measurement. We measured Raman intensities of gaseous hydrogen and benzene vapor under the same experimental conditions and determined the absolute Raman cross section of benzene vapor. The ontained absolute Raman cross section agreed well with the literature values after the correction for the dielectric field effect.

Research Products

• [Journal Article] Simultaneous imaging of fat crystallinity and crystal polymorphic types by Raman microspectroscopy. (2016)
  • Author(s): Michiyo Motoyama, Masahiro Ando, Keisuke Sasaki, Ikuyo Nakajima, Koichi Chikuni, Katsuhiro Aikawa, Hiro-o Hamaguchi
  • Journal Title: Food chemistry Volume: 196 Pages: 411-417
  • DOI: 10.1016/j.foodchem.2015.09.043
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Automatic and objective oral cancer diagnosis by Raman spectroscopic detection of keratin with multivariate curve resolution analysis (2016)
  • Author(s): P.-H. Chen, R. Shimada, S. Yabumoto, H. Okajima, M. Ando, C.-T. Chang, L.-T. Lee, Y.-K. Wong, A. Chiou, H. Hamaguchi
  • Journal Title: Scientific Reports Volume: 6 Issue: 1 Pages: 20097-20097
  • DOI: 10.1038/srep20097
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] 1.In Vivo Live Cell Imaging for the Quantitative Monitoring of Lipids by Using Raman Microspectroscopy (2014)
  • Author(s): Masahito Hosokawa, Masahiro Ando, Shoichiro Mukai, Kyoko Osada, Tomoko Yoshino, Hiro-o Hamaguchi and Tsuyoshi Tanaka
  • Journal Title: Analytical Chemistry Volume: 86 Issue: 16 Pages: 8224-8230
  • DOI: 10.1021/ac501591d
  • Peer Reviewed
• [Journal Article] 3.In Situ Detection of Antibiotic Amphotericin B Produced in Streptomyces nodosus Using Raman Microspectroscopy (2014)
  • Author(s): Rimi Miyaoka, Masahito Hosokawa, Masahiro Ando, Tetsushi Mori, Hiro-o Hamaguchi and Haruko Takeyama
  • Journal Title: Marine Drugs Volume: 12 Issue: 5 Pages: 2827-2839
  • DOI: 10.3390/md12052827
  • Peer Reviewed / Open Access
• [Journal Article] 4.Surfactant Uptake Dynamics in Mammalian Cells Elucidated with Quantitative Coherent Anti-Stokes Raman Scattering Microspectroscopy (2014)
  • Author(s): Masanari Okuno, Hideaki Kano, Kenkichi Fujii, Kotatsu Bito, Satoru Naito, Philippe Leproux, Vincent Couderc and Hiro-o Hamaguchi
  • Journal Title: Plos One Volume: 9 Issue: 4 Pages: e93401-e93401
  • DOI: 10.1371/journal.pone.0093401
  • Peer Reviewed
• [Journal Article] Molecular component distribution imaging of living cells by multivariate curve resolution analysis of space-resolved Raman spectra (2013)
  • Author(s): Masahiro Ando and Hiro-o Hamaguchi
  • Journal Title: Journal of Biomedical Optics Volume: 19 Issue: 1 Pages: 11016-11016
  • DOI: 10.1117/1.jbo.19.1.011016
  • Peer Reviewed
• [Journal Article] Quantitative analysis of the redox states of cytochromes in a living L929 (NCTC) cell by resonance Raman microspectroscopy (2013)
  • Author(s): Minoru Kakita, Masanari Okuno, and Hiro-o Hamaguchi
  • Journal Title: Journal of Biophtonics Volume: 6 Issue: 3 Pages: 256-259
  • DOI: 10.1002/jbio.201200042
  • Peer Reviewed
• [Presentation] Raman Big Data Analysis for Automatic and Objective Living Cell Discrimination/Diagnosis (2016)
  • Author(s): Hiro-o Hamaguchi
  • Organizer: Pittcon Conference and EXPO 2016
  • Place of Presentation: ,Atlanta
  • Year and Date: 2016-03-05
  • Int'l Joint Research / Invited
• [Presentation] Reading Molecular Fingerprints More Wisely (2015)
  • Author(s): Masahiro Ando, Soshi Yabumoto and Hiro-o Hamaguchi
  • Organizer: Pacifichem 2015
  • Place of Presentation: Honolulu
  • Year and Date: 2015-12-14
  • Int'l Joint Research / Invited
• [Presentation] Automatic and Objective Cell Discrimination by Raman Spectroscopy with Multivariate Curve Resolution (2015)
  • Author(s): Masahiro Ando and Hiro-o Hamaguchi
  • Organizer: The 5th Asian Spectroscopy Conference
  • Place of Presentation: Sydney
  • Year and Date: 2015-09-29
  • Int'l Joint Research / Invited
• [Presentation] 1.Fast, Automatic and Label-free Cell Discrimination by Raman Microspectroscopy (2014)
  • Author(s): Hiro-o Hamaguchi
  • Organizer: Optics and Photonics Taiwan International Conference
  • Place of Presentation: Taipei
  • Year and Date: 2014-12-04
  • Invited
• [Presentation] 2.Molecular Fingerprinting and mapping of living cells by Raman microspectroscopy, Hiro-o Hamaguchi (2014)
  • Author(s): Hiro-o Hamaguchi
  • Organizer: Advances in Live Single-cell Thermal Imaging and Manipulation, Okinawa Institute of Science and Technology Symposium
  • Place of Presentation: Okinawa
  • Year and Date: 2014-11-12
  • Invited
• [Presentation] Recent Advances in Raman Spectral Imaging of Living Cells: Development at Tokyo and Hsinchu (2013)
  • Author(s): Hiro-o Hamaguchi
  • Organizer: The 4th Asian Spectroscopy Conference
  • Place of Presentation: Singapore
  • Invited
• [Presentation] Diversity and Future of Raman Spectroscopy: What Can We Look at by Spontaneous Raman and Other Analogues of Raman Scattering? (2013)
  • Author(s): Hiro-o Hamaguchi
  • Organizer: The 7th International Conference on Advanced Vibrational Spectroscopy
  • Place of Presentation: Kobe
  • Invited
• [Book] ラマン分光法 (2015)
  • Author(s): 濵口宏夫、岩田耕一
  • Total Pages: 205
  • Publisher: 講談社サイエンティフィク
• [Book] ラマン分光法 (2015)
  • Author(s): 濵口宏夫、岩田耕一共編著
  • Total Pages: 205
  • Publisher: 講談社サイエンティフィク