Development of the hybrid separation system containing nano-bubbles as key components

• Project/Area Number: 25288062
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Analytical chemistry
• Research Institution: Saitama University
• Principal Investigator: SHIBUKAWA Masami 埼玉大学, 理工学研究科, 教授 (60148088)
• Co-Investigator(Kenkyū-buntansha): SAITO SHINGO 埼玉大学, 大学院理工学研究科, 准教授 (60343018)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000); Fiscal Year 2013: ¥10,010,000 (Direct Cost: ¥7,700,000、Indirect Cost: ¥2,310,000)
• Keywords: ナノバブル / 疎水性ナノ細孔 / 界面水 / ハイブリッド固定相 / HPLC / 表面気泡変調液体クロマトグラフィー

Outline of Final Research Achievements

A new chromatographic method termed surface-bubble-modulated liquid chromatography, that has a hybrid separation medium incorporated with surface nanobubbles, was developed. Nanobubbles can be fixed at the interface between water and a hydrophobic material by delivering water into a dry column packed with a nanoporous material. The incorporation of a gas phase leads to the formation of the hybrid separation system consisting of the gas phase, hydrophobic moieties, and the water/hydrophobic interface or the interfacial water. One can change the volume of the gas phase by pressure applied to the column, which in turn alters the area of water/hydrophobic interface, while the amount of the hydrophobic moiety remains constant. Therefore this strategy provides a novel technique not only for manipulating the separation selectivity by pressure but also for elucidating the mechanism of accumulation or retention of solute compounds in aqueous solutions by a hydrophobic material.

Research Products

• [Journal Article] Determination of trace amounts of aluminum in environmental and biological fluids by reversed-phase ion-pair liquid chromatography with fluorometric detection using 5-sulfoquinoline-8-ol (2016)
  • Author(s): Masami Shibukawa, Kazuhiko Koyahara, Ai Mishina, Kazunori Saitoh, Atsuko Nishigaki
  • Journal Title: Current Chromatography Volume: 3 Issue: 2 Pages: 1-6
  • DOI: 10.2174/2213240603666160301213923
  • Peer Reviewed / Open Access
• [Journal Article] Determination of the cis-trans Isomerization Barriers of L-Alanyl-L-Proline in Aqueous Solutions and at Water/Hydrophobic Interfaces by On-Line Temperature-Jump Relaxation HPLC and Dynamic On-Column Reaction HPLC (2015)
  • Author(s): Masami Shibukawa, Ayaka Miyake, Sayaka Eda, Shingo Saito
  • Journal Title: Analytical Chemistry Volume: 87 Issue: 18 Pages: 9280-9287
  • DOI: 10.1021/acs.analchem.5b02488
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Surface-bubble-modulated liquid chromatography: a new approach for manipulation of chromatographic retention and investigation of solute distribution at water/hydrophobic interfaces (2015)
  • Author(s): Keisuke Nakamura, Hiroki Nakamura, Shingo Saito, Masami Shibukawa
  • Journal Title: Analytical Chemistry Volume: 87 Issue: 2 Pages: 1180-1187
  • DOI: 10.1021/ac503802q
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] Evaluation of the intrinsic retention selectivity of hydrocarbonaceous and fluorocarbonaceous stationary phases by surface-bubble-modulated liquid chromatography (2015)
  • Author(s): K. Nakamura, S. Saito, M. Shibukawa
  • Organizer: Pacifichem 2015
  • Place of Presentation: Hawaii Convention Center, Honolulu, USA
  • Year and Date: 2015-12-16
  • Int'l Joint Research
• [Presentation] Surface-bubble-modulated liquid chromatography: A new strategy for manipulating chromatographic retention (2015)
  • Author(s): M. Shibukawa, K. Nakamura, S. Saito
  • Organizer: Pacifichem 2015
  • Place of Presentation: Marriott Resort Waikiki Beach Hotel, Honolulu, USA
  • Year and Date: 2015-12-15
  • Int'l Joint Research / Invited
• [Presentation] 超臨界二酸化炭素を固定相としたHPLCの開発 (2015)
  • Author(s): 山下貴大，齋藤伸吾，渋川雅美
  • Organizer: 日本分析化学会第64年会
  • Place of Presentation: 九州大学伊都キャンパス（福岡県福岡市）
  • Year and Date: 2015-09-10
• [Presentation] 液体クロマトグラフィー (2015)
  • Author(s): 渋川雅美
  • Organizer: 第42回湘南ハイテクセミナー
  • Place of Presentation: KUポートスクエア（神奈川県横浜市）
  • Year and Date: 2015-06-04
  • Invited
• [Presentation] 表面気泡変調液体クロマトグラフィーによる疎水性表面における水の分離機能の解析 (2015)
  • Author(s): 中村圭介，齋藤伸吾，渋川雅美
  • Organizer: 第75回分析化学討論会
  • Place of Presentation: 山梨大学工学部（山梨県甲府市）
  • Year and Date: 2015-05-23
• [Presentation] Surface bubble modulated liquid chromatography: a new approach for manipulating chromatographic retention (2015)
  • Author(s): Masami Shibukawa, Keisuke Nakamura, Shingo Saito
  • Organizer: Pittcon 2015 Conference and Expo
  • Place of Presentation: Ernst Morial Convention Center, New Orleans, U.S.A.
  • Year and Date: 2015-03-12
• [Presentation] Surface bubble modulated liquid chromatography: Retention mechanism and manipulation of the retention selectivity (2015)
  • Author(s): Keisuke Nakamura, Shingo Saito, Masami Shibukawa
  • Organizer: Pittcon 2015 Conference and Expo
  • Place of Presentation: Ernst Morial Convention Center, New Orleans, U.S.A.
  • Year and Date: 2015-03-12
• [Presentation] 表面ナノ気泡変調液体クロマトグラフィーの創案と開発 (2014)
  • Author(s): 渋川雅美
  • Organizer: 日本分析化学会第63年会
  • Place of Presentation: 広島大学東広島キャンパス（広島県東広島市）
  • Year and Date: 2014-09-17
  • Invited
• [Presentation] Surface bubble modulated liquid chromatography: Retention mechanism and separation efficiency (2014)
  • Author(s): Keisuke Nakamura, Shingo Saito, Masami Shibukawa
  • Organizer: RSC Tokyo International Conference 2014
  • Place of Presentation: 幕張メッセ（千葉県千葉市）
  • Year and Date: 2014-09-04
• [Presentation] Surface bubble modulated liquid chromatography: a new approach for manipulation of chromatographic retention and investigation of solute distribution at water/hydrophobic interfaces (2014)
  • Author(s): Masami Shibukawa, Keisuke Nakamura, Hiroki Nakamura, and Shingo Saito
  • Organizer: 2014 China-Japan-Korea Symposium on Analytical Chemistry
  • Place of Presentation: Northeastern University, Shenyang, China
  • Year and Date: 2014-08-24
  • Invited
• [Presentation] 気液ハイブリッド固定相分離システム構築のための気相形成方法の検討 (2014)
  • Author(s): 中村圭介，仲村大樹，齋藤伸吾，渋川雅美
  • Organizer: 第74回分析化学討論会
  • Place of Presentation: 日本大学工学部（福島県郡山市）
  • Year and Date: 2014-05-25
• [Presentation] Liquid chromatography with a Gaseous Stationary Phase
  • Author(s): K. Nakamura, M. Shibukawa
  • Organizer: 2013 China-Japan-Korea Symposium on Analytical Chemistry
  • Place of Presentation: 九州大学（福岡）
• [Presentation] 気液ハイブリッド固定相分離システム構築のための気相形成方法の検討
  • Author(s): 中村圭介，仲村大樹，齋藤伸吾，渋川雅美
  • Organizer: 第73回分析化学討論会
  • Place of Presentation: 北海道大学（函館）
• [Remarks] Shibukawa Laboratory
  • URL: http://www.apc.saitama-u.ac.jp/bunseki/index.html
• [Remarks] 埼玉大学 理工学研究科 渋川研究室 （分析化学）
  • URL: http://www.apc.saitama-u.ac.jp/bunseki/index.html
• [Remarks] 埼玉大学 理工学研究科 渋川研究室 （分析化学）
  • URL: http://www.apc.saitama-u.ac.jp/bunseki/index.html