Analytical System for Imaging Mass Spectrometry using Gold Nanoparticles as a Mass Probe

• Project/Area Number: 25288066
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Analytical chemistry
• Research Institution: Kagoshima University
• Principal Investigator: Niidome Yasuro 鹿児島大学, 学術研究院理工学域理学系, 准教授 (50264081)
• Co-Investigator(Kenkyū-buntansha): Niidome Takuro 熊本大学, 大学院自然科学研究科, 教授 (20264210)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000); Fiscal Year 2015: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2014: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000); Fiscal Year 2013: ¥12,090,000 (Direct Cost: ¥9,300,000、Indirect Cost: ¥2,790,000)
• Keywords: 金ナノロッド / 質量分析 / マスプローブ / 質量分析イメージング / イメージング質量分析 / 凝集状態制御 / ナノ材料 / ナノバイオ

Outline of Final Research Achievements

Liver tissue sections were prepared from mice in which gold nanorods were intravenously injected. Desorption of Gold ions from a 20 micrometer-thick section was possible. Imaging mass spectrometry of gold ions revealed the distribution of gold nanoparticles in tumor tissues of tumor-bearing mice. Gold nanoparticles were find in the areas where blood flow existed. It was found that gold nanorods acted as a mass probe in a living body.
Very sensitive detection of gold ions desorbed from polymer membranes was possible. However, large scale aggregation of nanoparticles suppressed the ionization/desorption of gold ions. Individually isolated gold nanorods were found to act as an efficient mass probe.

Research Products

• [Journal Article] Anisotropic Gold-Based Nanoparticles: Their Preparation, Properties, and Applications (2016)
  • Author(s): Yasuro Niidome, Aung Thu Haine, TakuroNiidome
  • Journal Title: Chemistry Letters Volume: 45 Issue: 5 Pages: 488-498
  • DOI: 10.1246/cl.160124
  • NAID: 130005150482
  • Peer Reviewed / Open Access
• [Journal Article] Assemblies of Gold Nanorods for Efficient SALDI Mass Spectrometry (2016)
  • Author(s): Masanori Fujii, Naotoshi Nakashima, Yasuro Niidome
  • Journal Title: Optical Materials Express Volume: 6 Issue: 4 Pages: 1376-1383
  • DOI: 10.1364/ome.6.001376
  • Peer Reviewed / Open Access
• [Journal Article] 銀シェル金ナノロッドの分光特性と基板固定 (2015)
  • Author(s): 新留康郎
  • Journal Title: 分光研究 Volume: 64 Pages: 514-521
  • NAID: 40020674968
  • Peer Reviewed
• [Journal Article] Imaging Mass Spectrometry of Gold Nanorods Distributed in Tumor Tissue (2015)
  • Author(s): Takuro Niidome, Masanori Fujii, Naotoshi Nakashima, Yoshiki Katayama, Yasuro Niidome
  • Journal Title: Chemistry Letters Volume: 44 Issue: 7 Pages: 931-933
  • DOI: 10.1246/cl.150258
  • NAID: 130005085146
  • Peer Reviewed / Open Access
• [Journal Article] In situ observation of structural transformation of gold nanorods under pulsed laser irradiation in an HVEM (2014)
  • Author(s): Nao Sumimoto, Koichiro Nakao, Tomokazu Yamamoto, Kazuhiro Yasuda, Syo Matsumura, and Yasuro Niidome
  • Journal Title: Microscopy Volume: 63 Issue: 4 Pages: 261-268
  • DOI: 10.1093/jmicro/dfu012
  • Peer Reviewed
• [Journal Article] 銀シェル金ナノロッド固定ITO基板の顕微分光特性とSEM観察 (2014)
  • Author(s): 新留康郎・鶴由貴子・濱崎祐樹・中嶋直敏
  • Journal Title: 分析化学 Volume: 63 Pages: 857-865
  • NAID: 130004712993
  • Peer Reviewed
• [Journal Article] Imaging Mass Spectrometry of Intravenously Injected Gold Nanorods in Mice (2014)
  • Author(s): Masanori Fujii, Naotoshi Nakashima, Takuro Niidome, and Yasuro Niidome
  • Journal Title: Chemistry Letters Volume: 43 Issue: 1 Pages: 131-133
  • DOI: 10.1246/cl.130864
  • NAID: 130004427094
  • Peer Reviewed
• [Journal Article] CW/pulsed NIR irradiation of gold nanorods: effect on transdermal protein delivery mediated by photothermal ablation (2013)
  • Author(s): H. Tang, H. Kobayashi, Y. Niidome, T. Mori, Y. Katayama, T. Niidome
  • Journal Title: J. Controlled Release Volume: 171 Issue: 2 Pages: 178-183
  • DOI: 10.1016/j.jconrel.2013.07.003
  • Peer Reviewed
• [Journal Article] Spontaneous Temperature Control Using Reversible Spectroscopic Responses of PNIPAM-coated Gold Nanorods (2013)
  • Author(s): Kohei Shimoda, Tsuyohiko Fujigaya, Naotoshi Nakashima, and Yasuro Niidome
  • Journal Title: Chemistry Letters Volume: Vol.42, No.10 Issue: 10 Pages: 1247-1249
  • DOI: 10.1246/cl.130457
  • NAID: 10031201326
  • Peer Reviewed
• [Journal Article] Electrochemical Oxidation of Silver Shells on Gold Nanorods in Potassium Chloride and Phosphate Buffer Solutions (2013)
  • Author(s): Yuki Hamasaki, Naotoshi Nakashima, and Yasuro Niidome
  • Journal Title: Chemistry Letters Volume: Vol.42, No.9 Issue: 9 Pages: 1093-1095
  • DOI: 10.1246/cl.130350
  • NAID: 10031194353
  • Peer Reviewed
• [Journal Article] Effects of Anions on Electrochemical Reactions of Silver Shells on Gold Nanorod (2013)
  • Author(s): Yuki Hamasaki
  • Journal Title: Journal of Physical Chemistry C Volume: 117 Issue: 6 Pages: 2521-2530
  • DOI: 10.1021/jp306469s
  • Peer Reviewed
• [Journal Article] Observation of Defocus Images of a Single Metal Nanorod (2013)
  • Author(s): 茂木俊憲
  • Journal Title: Journal of Physical Chemistry C Volume: 117 Issue: 6 Pages: 2535-2540
  • DOI: 10.1021/jp3065008
  • Peer Reviewed
• [Presentation] ポリマーメンブレン中の金ナノ粒子から金イオンの脱離イオン化 (2016)
  • Author(s): 新留 康郎、向 大輝
  • Organizer: 日本化学会第９６春季年会
  • Place of Presentation: 同志社大学（京都府京田辺市）
  • Year and Date: 2016-03-24
• [Presentation] 質量分析による金ナノ粒子の定量分析 (2016)
  • Author(s): 向 大輝
  • Organizer: 第５回サイエンスインカレ
  • Place of Presentation: 神戸国際会議場（兵庫県神戸市）
  • Year and Date: 2016-03-05
• [Presentation] Gold Nanorods as a Mass Probe: Imaging Mass Spectrometry of Gold Ions Desorbed from Tissue Section (2015)
  • Author(s): Yasuro Niidome、Daiki Muko、Masanori Fujii、Takuro Niidome
  • Organizer: 6th Asian Conference on Colloid and Interface Science (ACCIS 2015)
  • Place of Presentation: アルカス佐世保（長崎県佐世保市）
  • Year and Date: 2015-11-25
  • Int'l Joint Research
• [Presentation] Imaging Mass Spectrometry of Gold Nanoparticles in Blotting Membranes and Tissue Sections (2015)
  • Author(s): Daiki Muko、Yuki Inoue、Takuro Niidome、Yasuro Niidome
  • Organizer: 6th Asian Conference on Colloid and Interface Science (ACCIS 2015)
  • Place of Presentation: 佐世保（長崎県佐世保市）
  • Year and Date: 2015-11-25
  • Int'l Joint Research
• [Presentation] Colloidal Gold Nanoparticles Prepared in Hexadecyltrimethylammonium Bromide/Chloride Solutions (2015)
  • Author(s): Yuki Inoue、Yo Tsutamoto、Yasuro Niidome
  • Organizer: 6th Asian Conference on Colloid and Interface Science (ACCIS 2015)
  • Place of Presentation: 佐世保（長崎県佐世保市）
  • Year and Date: 2015-11-25
  • Int'l Joint Research
• [Presentation] Mass Spectrometry of Gold Colloids in Tissue Sections and Blotting Membranes (2015)
  • Author(s): Yasuro Niidome、Daiki Muko、Masanori Fujii
  • Organizer: West Pacific Colloid Meeting 2015 (WPC2015)
  • Place of Presentation: Shrim Riap, Cambodia
  • Year and Date: 2015-11-17
  • Int'l Joint Research
• [Presentation] ブロッティングメンブレン中の金ナノ粒子の脱離・イオン化効率 (2015)
  • Author(s): 向 大輝、井上 雄貴、新留 康郎
  • Organizer: 第６６回コロイドおよび界面化学討論会
  • Place of Presentation: 鹿児島大学（鹿児島県鹿児島市）
  • Year and Date: 2015-09-10
• [Presentation] カチオン性球状金ナノ粒子の調製とキャラクタリゼーション (2015)
  • Author(s): 井上 雄貴、向 大輝、蔦本 陽、新留 康郎
  • Organizer: 第６６回コロイドおよび界面化学討論会
  • Place of Presentation: 鹿児島大学（鹿児島県鹿児島市）
  • Year and Date: 2015-09-10
• [Presentation] Mapping of Gold Nanorods in a Liver Tissue Sections using Imaging Mass Spectrometry (2015)
  • Author(s): Yasuro Niidome、Masanori Fujii、Yuki Inoue、Daiki Muko、Takuro Niidome
  • Organizer: Gold 2015
  • Place of Presentation: Cardiff, UK
  • Year and Date: 2015-07-26
  • Int'l Joint Research
• [Presentation] 各種金ナノ粒子の金イオン脱離イオン化効率 (2015)
  • Author(s): 向 大輝、井上 雄貴、蔦本 陽、新留 康郎
  • Organizer: 第５２回化学関連支部合同九州大会
  • Place of Presentation: 北九州国際会議場（福岡県北九州市）
  • Year and Date: 2015-07-04
• [Presentation] カチオン性界面活性剤中で調製する球状粒子 (2015)
  • Author(s): 井上 雄貴、蔦本 陽、向 大輝、新留 康郎
  • Organizer: 第５２回化学関連支部合同九州大会
  • Place of Presentation: 北九州国際会議場（福岡県北九州市）
  • Year and Date: 2015-07-04
• [Presentation] 組織切片・メンブレン・ITO基板上の金ナノ粒子の脱離イオン化挙動 (2015)
  • Author(s): 向 大輝、井上 雄貴、新留 康郎
  • Organizer: 質量分析総合討論会
  • Place of Presentation: つくば国際会議場（茨城県つくば市）
  • Year and Date: 2015-06-17
• [Presentation] 組織切片中に分布した金ナノロッドのイメージング質量分析 (2015)
  • Author(s): 新留 康郎、藤井 政徳、向 大輝、井上 雄貴、蔦本 陽、中嶋 直敏
  • Organizer: 第７５回分析化学討論会
  • Place of Presentation: 山梨大学（山梨県甲府市）
  • Year and Date: 2015-05-23
• [Presentation] カチオン性表面を有する球状金ナノ粒子の調製 (2015)
  • Author(s): 蔦本 陽、井上 雄貴、向 大輝、新留 康郎
  • Organizer: ナノ学会第１３回大会
  • Place of Presentation: 東北大学（宮城県仙台市）
  • Year and Date: 2015-05-11
• [Presentation] Imaging Mass Spectrometry of Gold Nanorods Distributing in a Liver Section of a Mouse (2014)
  • Author(s): Yasuro Niidome、Masanori Fujii、Naotoshi Nakashima
  • Organizer: MRS 2014 Fall Meeting
  • Place of Presentation: Hynes Convention Center, Boston (USA)
  • Year and Date: 2014-11-30 – 2014-12-03
• [Presentation] 金イオンの生体組織切片からのレーザー脱離挙動 (2014)
  • Author(s): 藤井 政徳、中嶋 直敏、新留 康郎
  • Organizer: 第６５回コロイドおよび界面化学討論会
  • Place of Presentation: 東京理科大神楽坂キャンパス（東京都新宿区）
  • Year and Date: 2014-09-03 – 2014-09-05
• [Presentation] Surface Enhanced Desorption/Ionization Mass spectrometry using Gold Nanorod Aggregates (2014)
  • Author(s): Masanori Fujii、Naotoshi Nakashima、Yasuro Niidome
  • Organizer: The 15th IUMRS-International Conference in Asia (IUMRS-ICA 2014)
  • Place of Presentation: 福岡大学七隈キャンパス（福岡市城南区）
  • Year and Date: 2014-08-24 – 2014-08-30
• [Presentation] 銀シェル金ナノロッドの分光特性制御 (2014)
  • Author(s): 新留 康郎
  • Organizer: 第６回プラズモニック化学シンポジウム
  • Place of Presentation: 早稲田大学西早稲田キャンパス（東京都新宿区）
  • Year and Date: 2014-06-20
  • Invited
• [Presentation] 金ナノロッド上に成長する銀シェルの電気化学的制御 (2014)
  • Author(s): 濱崎 祐樹、中嶋 直敏、新留 康郎
  • Organizer: ナノ学会第１２回大会
  • Place of Presentation: 京都大学宇治キャンパス（京都府宇治市）
  • Year and Date: 2014-05-22 – 2014-05-24
• [Presentation] 質量分析イメージングによる金ナノ粒子の体内分布解析 (2014)
  • Author(s): 藤井 政徳、中嶋 直敏、新留 琢郎、新留 康郎
  • Organizer: ナノ学会第１２回大会
  • Place of Presentation: 京都大学宇治キャンパス（京都府宇治市）
  • Year and Date: 2014-05-22 – 2014-05-24
• [Presentation] 異方性金ナノ粒子をプローブに用いるイメージング質量分析 (2014)
  • Author(s): 藤井 政徳、中嶋 直敏、新留 康郎
  • Organizer: 第６２回質量分析総合討論会
  • Place of Presentation: ホテル阪急エキスポパーク（大阪府吹田市）
  • Year and Date: 2014-05-14 – 2014-05-16
• [Presentation] 金ナノロッド上に析出した銀シェルの電気化学的形状変化 (2014)
  • Author(s): 濱崎 祐樹、中嶋 直敏、新留 康郎
  • Organizer: 第94回日本化学会春季年会
  • Place of Presentation: 名古屋大学
• [Presentation] Aggregates of metal nanoparticles for efficient laser-induced desorption/ionization processes (2013)
  • Author(s): Masanori Fujii、Naotoshi Nakashima、Yasuro Niidome
  • Organizer: The 4th Asian Symposium on Advanced Materials (ASAM-4)
  • Place of Presentation: 台北、台湾
• [Presentation] Silver-Shelled Gold Nanorods Standing on an ITO Plate (2013)
  • Author(s): Yasuro Niidome
  • Organizer: The 4th Asian Symposium on Advanced Materials (ASAM-4)
  • Place of Presentation: 台北、台湾
• [Presentation] Exchanges of sodium cholate and DNAs with different chain length on single-walled carbon nanotubes (2013)
  • Author(s): Ayaka Inoue、Yasuro Niidome、Naotoshi Nakashima
  • Organizer: The 4th Asian Symposium on Advanced Materials (ASAM-4)
  • Place of Presentation: 台北、台湾
• [Presentation] 金属ナノ粒子の凝集状態がレーザー誘起脱離イオン化に与える影響 (2013)
  • Author(s): 藤井 政徳、中嶋 直敏、新留 康郎
  • Organizer: 第６４回コロイドおよび界面化学討論会
  • Place of Presentation: 名古屋工業大学
• [Presentation] ITO基板上で配向・凝集した銀シェル金ナノロッドの分光特性 (2013)
  • Author(s): 新留 康郎、濱崎 祐樹、鶴 由貴子、中嶋 直敏
  • Organizer: 第６４回コロイドおよび界面化学討論会
  • Place of Presentation: 名古屋工業大学
• [Presentation] カーボンナノチューブ表面におけるコール酸ナトリウムとオリゴDNAの置換反応の熱力学的解析 (2013)
  • Author(s): 井上 彩花、加藤 雄一、新留 康郎、中嶋 直敏
  • Organizer: 第６４回コロイドおよび界面化学討論会
  • Place of Presentation: 名古屋工業大学
• [Presentation] 電気化学的酸化における銀シェル金ナノロッドの形状変化プロセスの解明 (2013)
  • Author(s): 濱崎 祐樹、中嶋 直敏、新留 康郎
  • Organizer: 第６４回コロイドおよび界面化学討論会
  • Place of Presentation: 名古屋工業大学
• [Presentation] Effects of Anions on Oxidation of Au-Ag Core-Shell Nanorods (2013)
  • Author(s): Yuki Hamasaki、Naotoshi Nakashima、Yasuro Niidome
  • Organizer: JSAP-OSA Joint Symposia 2013 (The 74th JSAP Autumn Meeting 2013)
  • Place of Presentation: 同志社大学
• [Presentation] Spectroscopy of Silver-Shelled Gold Nanorods Standing on an ITO Plate (2013)
  • Author(s): Yasuro Niidome、Yukiko Tsuru、Naotoshi Nakashima
  • Organizer: Seventh International Conferenece on Advanved Vibrational Spectroscopy (ICAVS-6)
  • Place of Presentation: 神戸国際会議場
• [Presentation] 単層カーボンナノチューブ上でのコール酸ナトリウムと DNA の置換反応に対する熱力学的解析 (2013)
  • Author(s): 新留 康郎、濱崎 祐樹、鶴 由貴子、中嶋 直敏
  • Organizer: ナノ学会第１１回大会
  • Place of Presentation: 東京工業大学
• [Presentation] 銀シェル金ナノロッドの溶解析出に与えるアニオンの影響 (2013)
  • Author(s): 濱崎 祐樹、中嶋 直敏、新留 康郎
  • Organizer: 第73回分析化学討論会
  • Place of Presentation: 北大函館キャンパス
• [Presentation] 異方性金属ナノ粒子の凝集状態とSALDI脱離イオン化の相関 (2013)
  • Author(s): 藤井 政徳、中嶋 直敏、新留 康郎
  • Organizer: 第73回分析化学討論会
  • Place of Presentation: 北大函館キャンパス
• [Presentation] 酵素反応を用いた銀シェル金ナノロッドの酸化制御 (2013)
  • Author(s): 新留 康郎、鶴 由貴子、木谷 綾花、中嶋 直敏
  • Organizer: 第73回分析化学討論会
  • Place of Presentation: 北大函館キャンパス
• [Remarks] nanorod.net
  • URL: http://www.nanorod.net
• [Remarks] Nanorod LAB
  • URL: http://www.nanorod.net
• [Patent(Industrial Property Rights)] ナノ粒子脱離イオンプローブ質量分析 (2013)
  • Inventor(s): 新留康郎
  • Industrial Property Rights Holder: 新留康郎
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2013-126186
  • Filing Date: 2013-06-15