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Functional and Structural Characterization of Electron Transfer Reactions in Mitochondria Respiratory Chain Utilizing Nanodisc

Research Project

Project/Area Number 25288072
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Bio-related chemistry
Research InstitutionHokkaido University

Principal Investigator

Ishimori Koichiro  北海道大学, 理学(系)研究科(研究院), 教授 (20192487)

Co-Investigator(Kenkyū-buntansha) Takeshi Uchida  北海道大学, 大学院理学研究院, 准教授 (30343742)
Tomohide Saio  北海道大学, 大学院理学研究院, 助教 (80740802)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000)
Fiscal Year 2015: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2013: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
Keywordsシトクロムc酸化酵素 / シトクロムc / ナノディスク / 電子伝達反応 / ヘム / MSP / ドッキングシミュレーション / 構造揺らぎ / 生体膜モデル / シトクロム酸化酵素 / 電子伝達系
Outline of Final Research Achievements

We reconstituted cytochrome c oxidase (CcO) into a biomembrane model, nanodisc, to characterize its functions in the absence of detergents. The resonance Raman spectra revealed that the reconstitution of CcO into the nanodisc increases the oxygen affinity, leading to efficient reduction of dioxygen to water molecules. To discuss interactions regulating the electron transfer (ET) reactions, we examined the cytochrome c (Cyt c) interaction site on CcO by docking simulation. Unexpectedly, electrostatic interactions do not contribute to the stabilization of the complex, but regulate the binding orientation of Cyt c on CcO. Instead, hydrophobic interactions are the primary factors to stabilize the complex, and the dehydration associated with the formation of the hydrophobic interactions is the key process to facilitate the complex formation. On the other hand, the structural fluctuations are suppressed in the CcO interaction site on Cyt c, which would also characterize the ET reaction.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • Research Products

    (8 results)

All 2016 2015 2014 2013 Other

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Acknowledgement Compliant: 1 results) Presentation (6 results) (of which Int'l Joint Research: 2 results,  Invited: 6 results) Remarks (1 results)

  • [Journal Article] Investigation of the Redox-dependent Modulation of Structure and Dynamics in Human Cytochrome c2016

    • Author(s)
      Imai, M., Saio, T., Kumata, H., Uchida, T., Inagaki, F., Ishimori, K.
    • Journal Title

      Biochemical and Biophysical Research Communication

      Volume: 469 Pages: 978-984

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] Osmotic pressure effects reveal a specific dehydration-induced hydrophobic electron transfer structure comprising cytochrome c and cytochrome c oxidase2015

    • Author(s)
      Koichiro Ishimori
    • Organizer
      Pacifichem 2015
    • Place of Presentation
      ホノルル(アメリカ合衆国)
    • Year and Date
      2015-12-17
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Analysis of interactions in the electron transfer complex between Cytochrome c and Cytochrome c Oxidase using osmotic pressure2015

    • Author(s)
      Koichiro Ishimori
    • Organizer
      227th ECS Meeting
    • Place of Presentation
      シカゴ(アメリカ合衆国)
    • Year and Date
      2015-05-26
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Electron Transfer Mechanism from Cytochrome c to Cytochrome c Oxidase in Mitochondrial Respiratory Chain2014

    • Author(s)
      Ishimori, K
    • Organizer
      International Conference on Porphyrin and Phathalocyanine
    • Place of Presentation
      Lutfi Kirdar Convention and Exhibition Centre (トルコ)
    • Year and Date
      2014-06-22 – 2014-06-27
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 構造変化による蛋白質間電子伝達の制御機構2013

    • Author(s)
      石森浩一郎
    • Organizer
      生物無機化学夏季セミナー
    • Place of Presentation
      阿字ヶ浦クラブ,茨城県
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 呼吸鎖末端におけるシトクロムcからシトクロムc酸化酵素への電子伝達機構-構造変化による電子伝達制御-2013

    • Author(s)
      石森浩一郎
    • Organizer
      第86回日本生化学会大会
    • Place of Presentation
      パシフィコ横浜,神奈川県
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Key Interactions and Regulation Mechanism for Electron Transfer from Cytochrome c to Cytochrome c Oxidase2013

    • Author(s)
      Koichiro Ishimori
    • Organizer
      Canic 2013
    • Place of Presentation
      The Stockey Centre, Canada
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Remarks] 発表論文からpickup

    • URL

      http://wwwchem.sci.hokudai.ac.jp/~stchem/pickup/

    • Related Report
      2015 Annual Research Report

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Published: 2013-05-21   Modified: 2019-07-29  

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