| Budget Amount *help |
¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
Fiscal Year 2015: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2014: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
Fiscal Year 2013: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
|
|---|
| Outline of Final Research Achievements |
Alkylation to DNA is one of the mechanisms on the anticancer drug. Some of the alkylating agents, for example, cisplatin, melphalan etc., are used as the anticancer drugs for clinical application. But these drugs did not have the selectivity to target genes, resulted in the serious side effects. The alkylated reactions with high selectivity to target genes have the potential for developing the new anticancer drugs without side effects. In our research, we attempted the development the site directed alkylation activated with proximity effects to a target base in the hydrophobic space. We designed the new alkylated probes activated by hydrogen bonding formation to a target base in hydrophobic space. These probes consisted of alkylating part, 2-amino-6-vinylpurine, and binding part, Hoechst, exhibited high selectivity to thymine. These probes are expected to apply for the new tool as a selective control of gene expression
|
