| Budget Amount *help |
¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
Fiscal Year 2015: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2014: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2013: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
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| Outline of Final Research Achievements |
Protein-protein interactions (PPIs) play a central role in the signaling pathways that regulate many cellular functions including proliferation, differentiation, and cell death, and have emerged as a new potential clinical targets in the post-genome era. In this study, we aimed to develop a series of mid-sized molecules, of which the molecular size roughly ranges from 600 to 2,000, to recognize the large and flat interfaces and disrupt and detect intracellular PPIs. The cell-permeable bivalent inhibitors of K-Ras-PPIs and the natural product-based fluorescent probes that detect interactions of 14-3-3 and the phospholigands were designed and evaluated in vitro and in cells.
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