Budget Amount *help |
¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2017: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2016: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2015: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2014: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2013: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
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Outline of Final Research Achievements |
We created an artificial biocatalyst capable of catalyzing the C-C bond formation. To enable the binding to the catalytic serine residue of the active site of a lipase, NHC precursor 1 bearing a phosphate group (transition-state analog) was synthesized in 13 steps. Artificial biocatalyst CALB-1 was obtained by mixing 1 with a lipase, and it catalyzed the asymmetric benzoin reaction. We also demonstrated that enzymatic properties such as enantioselectivity could be controlled by the chemical modification of the I287C variant of a lipase. We also found that the catalyst loading of NHC can be reduced under solvent-free conditions. Even solid-to-solid or solid-to-liquid conversions took place with low catalyst loading (0.2 mol%), and even semi-solid state organocatalysis took place.
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