| Budget Amount *help |
¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
Fiscal Year 2015: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2014: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2013: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
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| Outline of Final Research Achievements |
This research focused on the bio-analytical application of the fluorescent probes by exploiting the AM-contact (arena-metal ion) emission shift, which is induced by the direct contact between metal ion and fluorophore within their Wan-der Waals radii. In this research we successfully applied a cadmium ion complex to the ratiometric detection of hydrogen sulfide, an bioactive gas, in living cells. We also designed the fluorescent probes appended with a cyclic ligand, which selectively shows a large emission shift upon binding with silver ion. We also successfully applied AM-contat sensing to the ratiomettic detection of zinc ion by the elaborated design of the metal binding unit.
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