Investigation of mass transfer in vascular endothelial cells by utilizing high-speed confocal PIV techqniue

• Project/Area Number: 25289028
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Fluid engineering
• Research Institution: Tokyo Institute of Technology (2015); The University of Tokyo
• Principal Investigator: SUGII Yasuhiko 東京工業大学, 理工学研究科, 産学官連携研究員 (90345108)
• Co-Investigator(Kenkyū-buntansha): MOGI Katsuo 東京工業大学, 理工学研究科, 助教 (20610950) ; HISHIDA Koichi 慶應義塾大学, 理工学部, 教授 (40156592)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000); Fiscal Year 2015: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2014: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2013: ¥8,840,000 (Direct Cost: ¥6,800,000、Indirect Cost: ¥2,040,000)
• Keywords: バイオ流体 / マイクロ流体 / 流体計測 / 共焦点マイクロPIV / 血管内皮細胞 / グリコカリックス / 流体工学 / 超解像度LIF / グリコカリックス層 / 血流 / バイオ流体力学

Outline of Final Research Achievements

In order to clarify Glycocalyx layer on vascular endothelial cells, a high resolution measurement technique for three-dimensional velocity distribution was developed by utilizing stealth fluorescent silica particles with a diameter of 100 nm coated with long chains of polyethylene glycol.. The developed technique was applied to endothelial cell cultured in a microfluidic device.

Research Products

• [Journal Article] Measurement of concentration distribution in endothelial surface layer using super resolution LIF technique (2016)
  • Author(s): 茂木 克雄，兼高 紀香，杉井 康彦，菱田 公一
  • Journal Title: Transactions of the JSME (in Japanese) Volume: 82 Issue: 833 Pages: 15-00404-15-00404
  • DOI: 10.1299/transjsme.15-00404
  • NAID: 130005121231
  • ISSN: 2187-9761
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] 超解像度法を用いた血管内皮細胞近傍の物質濃度分布計測 (2015)
  • Author(s): 茂木 克雄，兼高 紀香，大萱 晃大，杉井 康彦，菱田 公一
  • Organizer: 日本機械学会 第93期流体工学部門講演会
  • Place of Presentation: 東京理科大学（葛飾区・東京）
  • Year and Date: 2015-11-07
• [Presentation] Development of concentration measurement technique for evaluation of mass transfer in the glycocalyx layer (2015)
  • Author(s): Kanetaka N, Mogi K, Sugii Y, Hishida K
  • Organizer: the 11th International Symposium on Particle Image Velocimetry
  • Place of Presentation: Santa Barbara (USA)
  • Year and Date: 2015-09-14
  • Int'l Joint Research
• [Presentation] 血管内皮細胞グリコカリックス層内の高分子の超解像度濃度分布計測法の開発 (2015)
  • Author(s): 茂木 克雄，兼高 紀香，杉井 康彦，菱田 公一
  • Organizer: 第43回可視化情報シンポジウム
  • Place of Presentation: 工学院大学（新宿区・東京）
  • Year and Date: 2015-07-21
• [Presentation] Visualization of Near Surface Flow of Endothelial Cells based on Confocal Micro-PIV and Super-resolution Microscopy (2015)
  • Author(s): Mogi K, Sugii Y
  • Organizer: the 10th Pacific Symposium on Flow Visualization and Image Processing
  • Place of Presentation: Naples (Italy)
  • Year and Date: 2015-06-18
  • Int'l Joint Research
• [Presentation] 共焦点マイクロPIV と超解像度顕微鏡の組み合わせによる血管内皮細胞グリコカリックス層近傍の速度分布計測 (2014)
  • Author(s): 漆谷 真帆，杉井 康彦，茂木 克雄，菱田 公一
  • Organizer: 日本機械学会 第92期流体工学部門講演会
  • Place of Presentation: 富山県、富山大学
  • Year and Date: 2014-10-25 – 2014-10-26
• [Presentation] Investigation of Near Surface Flow Field and Glycocalyx Dimension of Endothelial Cells by Confocal Micro-PIV and Super-Resolution Microscopy (2014)
  • Author(s): M. Urushidani, Y. Sugii, K. Mogi, K. Hishida
  • Organizer: 17th Int. Symp. Applications of Laser Techniques to Fluid Mechanics
  • Place of Presentation: Lisbon, Portugal
  • Year and Date: 2014-07-07 – 2014-07-10
• [Presentation] Development of measurement technique for velocity field near endothelial glycocalyx layer by utilizing confocal micro-PIV and super-resolution microscopy (2014)
  • Author(s): M. Urushidani, K. Mogi, Y. Sugii, K. Hishida
  • Organizer: The 16th International Symposium on Flow Visualization
  • Place of Presentation: Okinawa Convention Center, Okinawa
  • Year and Date: 2014-06-24 – 2014-06-28
• [Presentation] 共焦点マイクロPIVと超解像度顕微鏡を用いた血管内皮細胞グリコカリックス層近傍の速度分布計測 (2014)
  • Author(s): 漆谷 真帆，杉井 康彦，茂木 克雄，菱田 公一
  • Organizer: 第37回日本バイオレオロジー学会年会
  • Place of Presentation: 埼玉県、大宮ソニックシティビル
  • Year and Date: 2014-06-05 – 2014-06-06
• [Presentation] Measurement of Three-Dimensional Velocity upon Endothelial Cells Utilizing Confocal Micro-PIV (2013)
  • Author(s): Y. Sugii, T. Mukouyama, K. Hishida
  • Organizer: The 10th International Symposium on Particle Image Velocimetry
  • Place of Presentation: Delft, Netherlands
• [Presentation] High Resolution Velocity Measurement around the Surface of Endothelial Cells by Confocal Particle Image Velocimetry (2013)
  • Author(s): N. Kanetaka, T. Mukouyama, K. Mogi, Y. Sugii, K. Hishida
  • Organizer: 7th Asian Pacific Conference on Biomechanics
  • Place of Presentation: Seoul, Korea
• [Presentation] 共焦点マイクロPIV/LIF 法を用いた血管内皮細胞，周りの速度・濃度分布計測法の開発 (2013)
  • Author(s): 兼高 紀香，向山 卓哉，杉井 康彦，菱田 公一
  • Organizer: 第50回日本伝熱シンポジウム
  • Place of Presentation: 宮城県、ウェスティンホテル仙台およびトラストシティカンファレンス・仙台
• [Presentation] 共焦点マイクロPIVを用いた血管内皮細胞近傍の速度分布計測法の開発 (2013)
  • Author(s): 向山 卓哉，杉井 康彦，菱田 公一
  • Organizer: 第41回可視化情報シンポジウム
  • Place of Presentation: 東京都、工学院大学
• [Presentation] 血管内皮細胞近傍の流動の高解像度計測法 (2013)
  • Author(s): 114. 杉井 康彦
  • Organizer: 日本機械学会 2013年度年次大会
  • Place of Presentation: 岡山県、岡山大学
  • Invited