| Project/Area Number |
25289172
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Civil and environmental engineering
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| Research Institution | Ehime University |
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Principal Investigator |
Watanabe Kozo 愛媛大学, 理工学研究科, 准教授 (80634435)
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| Co-Investigator(Kenkyū-buntansha) |
OMURA Tatsuo 東北大学, 未来科学技術共同研究センター, 教授 (30111248)
KATO Mikio 大阪府立大学, 高等教育推進機構, 教授 (30204499)
MIYAKE Yo 愛媛大学, 理工学研究科, 准教授 (90345801)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000)
Fiscal Year 2015: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2013: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
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| Keywords | DNA種分類 / 次世代シークエンシング / 底生動物 / 河川 / 種多様性 / 系統発生 / 系統進化 / 生態学 / 遺伝子 / 進化 / 生物多様性 / 土木環境システム |
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| Outline of Final Research Achievements |
Using high-throughput Next-generation Sequencing (NGS), we rapidly assessed species richness of stream macroinvertebrate communities among 10 sites in the Shigenobu River catchment in Ehime Prefecture Japan. A conventional morphological taxonomic identification found 64 taxa among 8921 individuals collected from the catchment. Our NGS analysis produced 165,508 reads of mitochondrial COI amplicons. Based on DNA barcoding analysis of the NGS data, 39,337 was hit and 128 species were identified. Further, we found a significant positive correlation between numbers of sequence reads and morphology-based abundance among 33 taxonomic groups (1 class, 3 orders, and 30 families). We also applied DNA taxonomy analysis using PTP model, and the presence of 298 species in the catchment was estimated.
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