| Project/Area Number |
25289176
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Civil and environmental engineering
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| Research Institution | National Institute of Public Health |
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Principal Investigator |
Akiba Michihiro 国立保健医療科学院, その他部局等, その他 (00159336)
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| Co-Investigator(Kenkyū-buntansha) |
岸田 直裕 国立保健医療科学院, その他部局等, その他 (10533359)
常田 聡 早稲田大学, 理工学術院, 教授 (30281645)
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| Research Collaborator |
FUJIMOTO Naoshi 東京農業大学, 応用生物科学部醸造科学科, 教授 (50297595)
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2015: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
Fiscal Year 2014: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2013: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
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| Keywords | 水道 / 微生物 / 遺伝子検査 |
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| Outline of Final Research Achievements |
In this study, we aimed to develop an exhaustive detection technique for viable waterborne bacteria by using fluorescence activated cell sorter (FACS) and next-generation sequencing (NGS). The developed FACS-NGS method was evaluated using process water samples collected in a drinking water treatment plant, comparing with a conventional cloning and Sanger sequencing (culture-dependent) method. As a result, microbial communities observed in the process water samples were different between the FACS-NGS method and culture-dependent method. Alphaproteobacteria was the major class determined by the culture-dependent method. In contrast, Alphaproteobacteria and Gammaproteobacteria were identified as the two major classes in the FACS-NGS method. The results suggested that the developed FACS-NGS method can detect diverse viable waterborne bacteria.
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